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Accurate Detection and Evaluation of the Gene-Editing Frequency in Plants Using Droplet Digital PCR

Gene-editing techniques are becoming powerful tools for modifying target genes in organisms. Although several methods have been reported that detect mutations at targeted loci induced by the CRISPR/Cas system in different organisms, they are semiquantitative and have difficulty in the detection of m...

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Detalles Bibliográficos
Autores principales: Peng, Cheng, Zheng, Ming, Ding, Lin, Chen, Xiaoyun, Wang, Xiaofu, Feng, Xuping, Wang, Junmin, Xu, Junfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767858/
https://www.ncbi.nlm.nih.gov/pubmed/33381141
http://dx.doi.org/10.3389/fpls.2020.610790