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Accurate Detection and Evaluation of the Gene-Editing Frequency in Plants Using Droplet Digital PCR
Gene-editing techniques are becoming powerful tools for modifying target genes in organisms. Although several methods have been reported that detect mutations at targeted loci induced by the CRISPR/Cas system in different organisms, they are semiquantitative and have difficulty in the detection of m...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767858/ https://www.ncbi.nlm.nih.gov/pubmed/33381141 http://dx.doi.org/10.3389/fpls.2020.610790 |