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Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum
Penicillium digitatum is the most damaging pathogen provoking green mold in citrus fruit during storage, and there is an urgent need for novel antifungal agents with high efficiency. The aim of this study was to investigate the antifungal effects of peptide thanatin against P. digitatum and the mole...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767931/ https://www.ncbi.nlm.nih.gov/pubmed/33381100 http://dx.doi.org/10.3389/fmicb.2020.606482 |
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author | Feng, Guirong Li, Xindan Wang, Wenjun Deng, Lili Zeng, Kaifang |
author_facet | Feng, Guirong Li, Xindan Wang, Wenjun Deng, Lili Zeng, Kaifang |
author_sort | Feng, Guirong |
collection | PubMed |
description | Penicillium digitatum is the most damaging pathogen provoking green mold in citrus fruit during storage, and there is an urgent need for novel antifungal agents with high efficiency. The aim of this study was to investigate the antifungal effects of peptide thanatin against P. digitatum and the molecular mechanisms. Results showed that peptide thanatin had a prominent inhibitory effect on P. digitatum by in vitro and in vivo test. A total of 938 genes, including 556 downregulated and 382 upregulated genes, were differentially expressed, as revealed by RNA-seq of whole P. digitatum genomes analysis with or without thanatin treatment. The downregulated genes mainly encoded RNA polymerase, ribosome biogenesis, amino acid metabolism, and major facilitator superfamily. The genes associated with heat shock proteins and antioxidative systems were widely expressed in thanatin-treated group. DNA, RNA, and the protein content of P. digitatum were significantly decreased after thanatin treatment. In conclusion, thanatin could inhibit the growth of P. digitatum, and the underlying mechanism might be the genetic information processing and stress response were affected. The research will provide more precise and directional clues to explore the inhibitory mechanism of thanatin on growth of P. digitatum. |
format | Online Article Text |
id | pubmed-7767931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77679312020-12-29 Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum Feng, Guirong Li, Xindan Wang, Wenjun Deng, Lili Zeng, Kaifang Front Microbiol Microbiology Penicillium digitatum is the most damaging pathogen provoking green mold in citrus fruit during storage, and there is an urgent need for novel antifungal agents with high efficiency. The aim of this study was to investigate the antifungal effects of peptide thanatin against P. digitatum and the molecular mechanisms. Results showed that peptide thanatin had a prominent inhibitory effect on P. digitatum by in vitro and in vivo test. A total of 938 genes, including 556 downregulated and 382 upregulated genes, were differentially expressed, as revealed by RNA-seq of whole P. digitatum genomes analysis with or without thanatin treatment. The downregulated genes mainly encoded RNA polymerase, ribosome biogenesis, amino acid metabolism, and major facilitator superfamily. The genes associated with heat shock proteins and antioxidative systems were widely expressed in thanatin-treated group. DNA, RNA, and the protein content of P. digitatum were significantly decreased after thanatin treatment. In conclusion, thanatin could inhibit the growth of P. digitatum, and the underlying mechanism might be the genetic information processing and stress response were affected. The research will provide more precise and directional clues to explore the inhibitory mechanism of thanatin on growth of P. digitatum. Frontiers Media S.A. 2020-12-14 /pmc/articles/PMC7767931/ /pubmed/33381100 http://dx.doi.org/10.3389/fmicb.2020.606482 Text en Copyright © 2020 Feng, Li, Wang, Deng and Zeng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Feng, Guirong Li, Xindan Wang, Wenjun Deng, Lili Zeng, Kaifang Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum |
title | Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum |
title_full | Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum |
title_fullStr | Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum |
title_full_unstemmed | Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum |
title_short | Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum |
title_sort | effects of peptide thanatin on the growth and transcriptome of penicillium digitatum |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767931/ https://www.ncbi.nlm.nih.gov/pubmed/33381100 http://dx.doi.org/10.3389/fmicb.2020.606482 |
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