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Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

BACKGROUND: Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. Due to technical limit...

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Detalles Bibliográficos
Autores principales: Zhang, Yang, Dai, Chunyang, Wang, Huiyan, Gao, Yong, Li, Tuantuan, Fang, Yan, Shen, Zuojun, Chen, Lichang, Chen, Zhaowu, Ma, Xuejun, Li, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768088/
https://www.ncbi.nlm.nih.gov/pubmed/33371898
http://dx.doi.org/10.1186/s12985-020-01467-y

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