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Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering

In recent years, there has been an increasing interest toward the covalent binding of bioactive peptides from extracellular matrix proteins on scaffolds as a promising functionalization strategy in the development of biomimetic matrices for tissue engineering. A totally new approach for scaffold fun...

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Autores principales: Rosellini, Elisabetta, Madeddu, Denise, Barbani, Niccoletta, Frati, Caterina, Graiani, Gallia, Falco, Angela, Lagrasta, Costanza, Quaini, Federico, Cascone, Maria Grazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768388/
https://www.ncbi.nlm.nih.gov/pubmed/33322426
http://dx.doi.org/10.3390/biomimetics5040067
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author Rosellini, Elisabetta
Madeddu, Denise
Barbani, Niccoletta
Frati, Caterina
Graiani, Gallia
Falco, Angela
Lagrasta, Costanza
Quaini, Federico
Cascone, Maria Grazia
author_facet Rosellini, Elisabetta
Madeddu, Denise
Barbani, Niccoletta
Frati, Caterina
Graiani, Gallia
Falco, Angela
Lagrasta, Costanza
Quaini, Federico
Cascone, Maria Grazia
author_sort Rosellini, Elisabetta
collection PubMed
description In recent years, there has been an increasing interest toward the covalent binding of bioactive peptides from extracellular matrix proteins on scaffolds as a promising functionalization strategy in the development of biomimetic matrices for tissue engineering. A totally new approach for scaffold functionalization with peptides is based on Molecular Imprinting technology. In this work, imprinted particles with recognition properties toward laminin and fibronectin bioactive moieties were synthetized and used for the functionalization of biomimetic sponges, which were based on a blend of alginate, gelatin, and elastin. Functionalized sponges underwent a complete morphological, physicochemical, mechanical, functional, and biological characterization. Micrographs of functionalized sponges showed a highly porous structure and a quite homogeneous distribution of imprinted particles on their surface. Infrared and thermal analyses pointed out the presence of interactions between blend components. Biodegradation and mechanical properties appeared adequate for the aimed application. The results of recognition tests showed that the deposition on sponges did not alter the specific recognition and binding behavior of imprinted particles. In vitro biological characterization with cardiac progenitor cells showed that early cell adherence was promoted. In vivo analysis showed that developed scaffolds improved cardiac progenitor cell adhesion and differentiation toward myocardial phenotypes.
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spelling pubmed-77683882020-12-29 Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering Rosellini, Elisabetta Madeddu, Denise Barbani, Niccoletta Frati, Caterina Graiani, Gallia Falco, Angela Lagrasta, Costanza Quaini, Federico Cascone, Maria Grazia Biomimetics (Basel) Article In recent years, there has been an increasing interest toward the covalent binding of bioactive peptides from extracellular matrix proteins on scaffolds as a promising functionalization strategy in the development of biomimetic matrices for tissue engineering. A totally new approach for scaffold functionalization with peptides is based on Molecular Imprinting technology. In this work, imprinted particles with recognition properties toward laminin and fibronectin bioactive moieties were synthetized and used for the functionalization of biomimetic sponges, which were based on a blend of alginate, gelatin, and elastin. Functionalized sponges underwent a complete morphological, physicochemical, mechanical, functional, and biological characterization. Micrographs of functionalized sponges showed a highly porous structure and a quite homogeneous distribution of imprinted particles on their surface. Infrared and thermal analyses pointed out the presence of interactions between blend components. Biodegradation and mechanical properties appeared adequate for the aimed application. The results of recognition tests showed that the deposition on sponges did not alter the specific recognition and binding behavior of imprinted particles. In vitro biological characterization with cardiac progenitor cells showed that early cell adherence was promoted. In vivo analysis showed that developed scaffolds improved cardiac progenitor cell adhesion and differentiation toward myocardial phenotypes. MDPI 2020-12-11 /pmc/articles/PMC7768388/ /pubmed/33322426 http://dx.doi.org/10.3390/biomimetics5040067 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosellini, Elisabetta
Madeddu, Denise
Barbani, Niccoletta
Frati, Caterina
Graiani, Gallia
Falco, Angela
Lagrasta, Costanza
Quaini, Federico
Cascone, Maria Grazia
Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering
title Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering
title_full Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering
title_fullStr Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering
title_full_unstemmed Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering
title_short Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering
title_sort development of biomimetic alginate/gelatin/elastin sponges with recognition properties toward bioactive peptides for cardiac tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768388/
https://www.ncbi.nlm.nih.gov/pubmed/33322426
http://dx.doi.org/10.3390/biomimetics5040067
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