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Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018

Ovine gammaherpesvirus-2 (OvHV-2) causes a lethal disease in cattle and some wild ruminants called malignant catarrhal fever (MCF), which affects the epithelial and lymphoid tissues of the respiratory and digestive tracts and has an important impact on the livestock industry. In this study, MCF was...

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Autores principales: Abd El Rahman, Sahar, Ateya, Ahmed, El-Beskawy, Mohamed, Wernike, Kerstin, Hoffmann, Bernd, Eschbaumer, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768452/
https://www.ncbi.nlm.nih.gov/pubmed/33322397
http://dx.doi.org/10.3390/vetsci7040201
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author Abd El Rahman, Sahar
Ateya, Ahmed
El-Beskawy, Mohamed
Wernike, Kerstin
Hoffmann, Bernd
Eschbaumer, Michael
author_facet Abd El Rahman, Sahar
Ateya, Ahmed
El-Beskawy, Mohamed
Wernike, Kerstin
Hoffmann, Bernd
Eschbaumer, Michael
author_sort Abd El Rahman, Sahar
collection PubMed
description Ovine gammaherpesvirus-2 (OvHV-2) causes a lethal disease in cattle and some wild ruminants called malignant catarrhal fever (MCF), which affects the epithelial and lymphoid tissues of the respiratory and digestive tracts and has an important impact on the livestock industry. In this study, MCF was diagnosed in 18 of 427 cattle from different sites in Egypt by its typical clinical signs, found in all 18 animals: corneal opacity, fever, erosions in the buccal cavity, lymphadenitis, and purulent nasal discharge. All affected cattle had been reared in contact with clinically inconspicuous sheep. Of the 18 clinically ill cattle, 13 succumbed to the disease, resulting in estimated morbidity and case fatality rates of 4.2% and 72.2%, respectively. Five samples collected from the affected cattle were positive for OvHV-2 by real-time PCR and were used for sequencing of an 832-bp fragment of the ORF27/gp48 gene. The ORF27 nucleotide sequence of all Egyptian samples was identical, but distinct from viruses found in other parts of Africa and the Mediterranean.
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spelling pubmed-77684522020-12-29 Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018 Abd El Rahman, Sahar Ateya, Ahmed El-Beskawy, Mohamed Wernike, Kerstin Hoffmann, Bernd Eschbaumer, Michael Vet Sci Article Ovine gammaherpesvirus-2 (OvHV-2) causes a lethal disease in cattle and some wild ruminants called malignant catarrhal fever (MCF), which affects the epithelial and lymphoid tissues of the respiratory and digestive tracts and has an important impact on the livestock industry. In this study, MCF was diagnosed in 18 of 427 cattle from different sites in Egypt by its typical clinical signs, found in all 18 animals: corneal opacity, fever, erosions in the buccal cavity, lymphadenitis, and purulent nasal discharge. All affected cattle had been reared in contact with clinically inconspicuous sheep. Of the 18 clinically ill cattle, 13 succumbed to the disease, resulting in estimated morbidity and case fatality rates of 4.2% and 72.2%, respectively. Five samples collected from the affected cattle were positive for OvHV-2 by real-time PCR and were used for sequencing of an 832-bp fragment of the ORF27/gp48 gene. The ORF27 nucleotide sequence of all Egyptian samples was identical, but distinct from viruses found in other parts of Africa and the Mediterranean. MDPI 2020-12-11 /pmc/articles/PMC7768452/ /pubmed/33322397 http://dx.doi.org/10.3390/vetsci7040201 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abd El Rahman, Sahar
Ateya, Ahmed
El-Beskawy, Mohamed
Wernike, Kerstin
Hoffmann, Bernd
Eschbaumer, Michael
Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018
title Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018
title_full Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018
title_fullStr Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018
title_full_unstemmed Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018
title_short Field Observations and Genetic Characterization of Sheep-Associated Malignant Catarrhal Fever in Egypt, 2018
title_sort field observations and genetic characterization of sheep-associated malignant catarrhal fever in egypt, 2018
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768452/
https://www.ncbi.nlm.nih.gov/pubmed/33322397
http://dx.doi.org/10.3390/vetsci7040201
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