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Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees

Acaricides and the gut parasite Nosema ceranae are commonly present in most productive hives. Those stressors could be affecting key semiochemicals, which act as homeostasis regulators in Apis mellifera colonies, such as cuticular hydrocarbons (CHC) involved in social recognition and ethyl oleate (E...

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Autores principales: Porrini, Martín Pablo, Garrido, Paula Melisa, Umpiérrez, María Laura, Porrini, Leonardo Pablo, Cuniolo, Antonella, Davyt, Belén, González, Andrés, Eguaras, Martín Javier, Rossini, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768465/
https://www.ncbi.nlm.nih.gov/pubmed/33302502
http://dx.doi.org/10.3390/vetsci7040199
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author Porrini, Martín Pablo
Garrido, Paula Melisa
Umpiérrez, María Laura
Porrini, Leonardo Pablo
Cuniolo, Antonella
Davyt, Belén
González, Andrés
Eguaras, Martín Javier
Rossini, Carmen
author_facet Porrini, Martín Pablo
Garrido, Paula Melisa
Umpiérrez, María Laura
Porrini, Leonardo Pablo
Cuniolo, Antonella
Davyt, Belén
González, Andrés
Eguaras, Martín Javier
Rossini, Carmen
author_sort Porrini, Martín Pablo
collection PubMed
description Acaricides and the gut parasite Nosema ceranae are commonly present in most productive hives. Those stressors could be affecting key semiochemicals, which act as homeostasis regulators in Apis mellifera colonies, such as cuticular hydrocarbons (CHC) involved in social recognition and ethyl oleate (EO) which plays a role as primer pheromone in honey bees. Here we test the effect of amitraz, coumaphos, tau-fluvalinate and flumethrin, commonly applied to treat varroosis, on honey bee survival time, rate of food consumption, CHC profiles and EO production on N. ceranae-infected and non-infected honey bees. Different sublethal concentrations of amitraz, coumaphos, tau-fluvalinate and flumethrin were administered chronically in a syrup-based diet. After treatment, purified hole-body extracts were analyzed by gas chromatography coupled to mass spectrometry. While N. ceranae infection was also shown to decrease EO production affecting survival rates, acaricides showed no significant effect on this pheromone. As for the CHC, we found no changes in relation to the health status or consumption of acaricides. This absence of alteration in EO or CHC as response to acaricides ingestion or in combination with N. ceranae, suggests that worker honey bees exposed to those highly ubiquitous drugs are hardly differentiated by nest-mates. Having determined a synergic effect on mortality in worker bees exposed to coumaphos and Nosema infection but also, alterations in EO production as a response to N. ceranae infection it is an interesting clue to deeper understand the effects of parasite-host-pesticide interaction on colony functioning.
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spelling pubmed-77684652020-12-29 Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees Porrini, Martín Pablo Garrido, Paula Melisa Umpiérrez, María Laura Porrini, Leonardo Pablo Cuniolo, Antonella Davyt, Belén González, Andrés Eguaras, Martín Javier Rossini, Carmen Vet Sci Article Acaricides and the gut parasite Nosema ceranae are commonly present in most productive hives. Those stressors could be affecting key semiochemicals, which act as homeostasis regulators in Apis mellifera colonies, such as cuticular hydrocarbons (CHC) involved in social recognition and ethyl oleate (EO) which plays a role as primer pheromone in honey bees. Here we test the effect of amitraz, coumaphos, tau-fluvalinate and flumethrin, commonly applied to treat varroosis, on honey bee survival time, rate of food consumption, CHC profiles and EO production on N. ceranae-infected and non-infected honey bees. Different sublethal concentrations of amitraz, coumaphos, tau-fluvalinate and flumethrin were administered chronically in a syrup-based diet. After treatment, purified hole-body extracts were analyzed by gas chromatography coupled to mass spectrometry. While N. ceranae infection was also shown to decrease EO production affecting survival rates, acaricides showed no significant effect on this pheromone. As for the CHC, we found no changes in relation to the health status or consumption of acaricides. This absence of alteration in EO or CHC as response to acaricides ingestion or in combination with N. ceranae, suggests that worker honey bees exposed to those highly ubiquitous drugs are hardly differentiated by nest-mates. Having determined a synergic effect on mortality in worker bees exposed to coumaphos and Nosema infection but also, alterations in EO production as a response to N. ceranae infection it is an interesting clue to deeper understand the effects of parasite-host-pesticide interaction on colony functioning. MDPI 2020-12-08 /pmc/articles/PMC7768465/ /pubmed/33302502 http://dx.doi.org/10.3390/vetsci7040199 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Porrini, Martín Pablo
Garrido, Paula Melisa
Umpiérrez, María Laura
Porrini, Leonardo Pablo
Cuniolo, Antonella
Davyt, Belén
González, Andrés
Eguaras, Martín Javier
Rossini, Carmen
Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees
title Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees
title_full Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees
title_fullStr Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees
title_full_unstemmed Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees
title_short Effects of Synthetic Acaricides and Nosema ceranae (Microsporidia: Nosematidae) on Molecules Associated with Chemical Communication and Recognition in Honey Bees
title_sort effects of synthetic acaricides and nosema ceranae (microsporidia: nosematidae) on molecules associated with chemical communication and recognition in honey bees
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768465/
https://www.ncbi.nlm.nih.gov/pubmed/33302502
http://dx.doi.org/10.3390/vetsci7040199
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