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CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway
OBJECTIVE: The present study aimed to evaluate the effects of cluster of differentiation (CD)4(+)CD25(+) forkhead box p3 (Foxp3)(+) regulatory T cells (Tregs) on unexplained recurrent spontaneous abortion (URSA) and the associated mechanisms. METHODS: The proportion of CD4(+)CD25(+)Foxp3(+) Tregs an...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768580/ https://www.ncbi.nlm.nih.gov/pubmed/33356705 http://dx.doi.org/10.1177/0300060520980940 |
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author | Qin, Shuang Li, Li Liu, Jia Zhang, Jinrui Xiao, Qing Fan, Yujuan Wei, Xiangcai |
author_facet | Qin, Shuang Li, Li Liu, Jia Zhang, Jinrui Xiao, Qing Fan, Yujuan Wei, Xiangcai |
author_sort | Qin, Shuang |
collection | PubMed |
description | OBJECTIVE: The present study aimed to evaluate the effects of cluster of differentiation (CD)4(+)CD25(+) forkhead box p3 (Foxp3)(+) regulatory T cells (Tregs) on unexplained recurrent spontaneous abortion (URSA) and the associated mechanisms. METHODS: The proportion of CD4(+)CD25(+)Foxp3(+) Tregs and inflammatory cytokine concentrations in the peripheral blood of women with URSA were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. CBA/JxDBA/2J mating was used to establish an abortion-prone mouse model and the model mice were treated with the Toll-like receptor 4 (TLR4) antagonist E5564 and the TLR4 agonist lipopolysaccharide. RESULTS: The proportion of CD4(+)CD25(+)Foxp3(+) Tregs was decreased and the inflammatory response was increased in women with URSA. In the abortion-prone mouse model, E5564 significantly increased the proportion of CD4(+)CD25(+)Foxp3(+) Tregs, decreased the inflammatory response, and increased Foxp3 mRNA and protein expression. Lipopolysaccharide had adverse effects on the abortion-prone model. CONCLUSIONS: These data suggest that CD4(+)CD25(+)Foxp3(+) Tregs regulate immune homeostasis in URSA via the TLR4/nuclear factor-κB pathway, and that the TLR4 antagonist E5564 may be a novel and potential drug for treating URSA. |
format | Online Article Text |
id | pubmed-7768580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-77685802021-01-21 CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway Qin, Shuang Li, Li Liu, Jia Zhang, Jinrui Xiao, Qing Fan, Yujuan Wei, Xiangcai J Int Med Res Prospective Clinical Research Report OBJECTIVE: The present study aimed to evaluate the effects of cluster of differentiation (CD)4(+)CD25(+) forkhead box p3 (Foxp3)(+) regulatory T cells (Tregs) on unexplained recurrent spontaneous abortion (URSA) and the associated mechanisms. METHODS: The proportion of CD4(+)CD25(+)Foxp3(+) Tregs and inflammatory cytokine concentrations in the peripheral blood of women with URSA were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. CBA/JxDBA/2J mating was used to establish an abortion-prone mouse model and the model mice were treated with the Toll-like receptor 4 (TLR4) antagonist E5564 and the TLR4 agonist lipopolysaccharide. RESULTS: The proportion of CD4(+)CD25(+)Foxp3(+) Tregs was decreased and the inflammatory response was increased in women with URSA. In the abortion-prone mouse model, E5564 significantly increased the proportion of CD4(+)CD25(+)Foxp3(+) Tregs, decreased the inflammatory response, and increased Foxp3 mRNA and protein expression. Lipopolysaccharide had adverse effects on the abortion-prone model. CONCLUSIONS: These data suggest that CD4(+)CD25(+)Foxp3(+) Tregs regulate immune homeostasis in URSA via the TLR4/nuclear factor-κB pathway, and that the TLR4 antagonist E5564 may be a novel and potential drug for treating URSA. SAGE Publications 2020-12-23 /pmc/articles/PMC7768580/ /pubmed/33356705 http://dx.doi.org/10.1177/0300060520980940 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Prospective Clinical Research Report Qin, Shuang Li, Li Liu, Jia Zhang, Jinrui Xiao, Qing Fan, Yujuan Wei, Xiangcai CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway |
title | CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway |
title_full | CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway |
title_fullStr | CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway |
title_full_unstemmed | CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway |
title_short | CD4(+)CD25(+)Foxp3(+) regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway |
title_sort | cd4(+)cd25(+)foxp3(+) regulatory t cells regulate immune balance in unexplained recurrent spontaneous abortion via the toll-like receptor 4/nuclear factor-κb pathway |
topic | Prospective Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768580/ https://www.ncbi.nlm.nih.gov/pubmed/33356705 http://dx.doi.org/10.1177/0300060520980940 |
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