Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site

Among the panel of monoclonal antibodies to the recombinant protein HlyIICTD Bacillus cereus an antibody was found capable of forming an immune complex with a thrombin recognition region, the amino acid sequence of which is located inside the recombinant HlyIICTD. Localization of the epitope was car...

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Autores principales: Zamyatina, A. V., Rudenko, N. V., Karatovskaya, A. P., Shepelyakovskaya, A. O., Siunov, A. V., Andreeva-Kovalevskaya, Zh. I., Nagel, A. S., Salyamov, V. I., Kolesnikov, A. S., Brovko, F. A., Solonin, A. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768993/
https://www.ncbi.nlm.nih.gov/pubmed/33390685
http://dx.doi.org/10.1134/S1068162020060382
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author Zamyatina, A. V.
Rudenko, N. V.
Karatovskaya, A. P.
Shepelyakovskaya, A. O.
Siunov, A. V.
Andreeva-Kovalevskaya, Zh. I.
Nagel, A. S.
Salyamov, V. I.
Kolesnikov, A. S.
Brovko, F. A.
Solonin, A. S.
author_facet Zamyatina, A. V.
Rudenko, N. V.
Karatovskaya, A. P.
Shepelyakovskaya, A. O.
Siunov, A. V.
Andreeva-Kovalevskaya, Zh. I.
Nagel, A. S.
Salyamov, V. I.
Kolesnikov, A. S.
Brovko, F. A.
Solonin, A. S.
author_sort Zamyatina, A. V.
collection PubMed
description Among the panel of monoclonal antibodies to the recombinant protein HlyIICTD Bacillus cereus an antibody was found capable of forming an immune complex with a thrombin recognition region, the amino acid sequence of which is located inside the recombinant HlyIICTD. Localization of the epitope was carried out using peptide phage display methods, as well as enzyme immunoassay and immunoblotting for interaction with recombinant proteins, either containing or not containing individual components HlyIICTD. The identified epitope is located in the region of the thrombin site and retains the ability to interact with the antibody after the proteolyotic attack of the protein by thrombin.
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spelling pubmed-77689932020-12-28 Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site Zamyatina, A. V. Rudenko, N. V. Karatovskaya, A. P. Shepelyakovskaya, A. O. Siunov, A. V. Andreeva-Kovalevskaya, Zh. I. Nagel, A. S. Salyamov, V. I. Kolesnikov, A. S. Brovko, F. A. Solonin, A. S. Russ J Bioorg Chem Article Among the panel of monoclonal antibodies to the recombinant protein HlyIICTD Bacillus cereus an antibody was found capable of forming an immune complex with a thrombin recognition region, the amino acid sequence of which is located inside the recombinant HlyIICTD. Localization of the epitope was carried out using peptide phage display methods, as well as enzyme immunoassay and immunoblotting for interaction with recombinant proteins, either containing or not containing individual components HlyIICTD. The identified epitope is located in the region of the thrombin site and retains the ability to interact with the antibody after the proteolyotic attack of the protein by thrombin. Pleiades Publishing 2020-12-28 2020 /pmc/articles/PMC7768993/ /pubmed/33390685 http://dx.doi.org/10.1134/S1068162020060382 Text en © Pleiades Publishing, Ltd. 2020, ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2020, Vol. 46, No. 6, pp. 1214–1220. © Pleiades Publishing, Ltd., 2020.Russian Text © The Author(s), 2020, published in Bioorganicheskaya Khimiya, 2020, Vol. 46, No. 6, pp. 753–760. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Zamyatina, A. V.
Rudenko, N. V.
Karatovskaya, A. P.
Shepelyakovskaya, A. O.
Siunov, A. V.
Andreeva-Kovalevskaya, Zh. I.
Nagel, A. S.
Salyamov, V. I.
Kolesnikov, A. S.
Brovko, F. A.
Solonin, A. S.
Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site
title Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site
title_full Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site
title_fullStr Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site
title_full_unstemmed Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site
title_short Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site
title_sort monoclonal antibody hlyiic‑15 to c-end domain hlyii b. cereus interacts with the trombin recognition site
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768993/
https://www.ncbi.nlm.nih.gov/pubmed/33390685
http://dx.doi.org/10.1134/S1068162020060382
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