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Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits

Ischemia-reperfusion injury is an important contributor to acute kidney injury and a major factor affecting early functional recovery after kidney transplantation. We conducted this experiment to investigate the protective effect of induced multipotent stem cell transplantation on renal ischemia-rep...

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Autores principales: Ruan, Guang-ping, Yao, Xiang, Lin, Qing-keng, Li, Zi-an, Cai, Xue-min, Pang, Rong-qing, Pan, Xing-hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769466/
https://www.ncbi.nlm.nih.gov/pubmed/33370374
http://dx.doi.org/10.1371/journal.pone.0244160
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author Ruan, Guang-ping
Yao, Xiang
Lin, Qing-keng
Li, Zi-an
Cai, Xue-min
Pang, Rong-qing
Pan, Xing-hua
author_facet Ruan, Guang-ping
Yao, Xiang
Lin, Qing-keng
Li, Zi-an
Cai, Xue-min
Pang, Rong-qing
Pan, Xing-hua
author_sort Ruan, Guang-ping
collection PubMed
description Ischemia-reperfusion injury is an important contributor to acute kidney injury and a major factor affecting early functional recovery after kidney transplantation. We conducted this experiment to investigate the protective effect of induced multipotent stem cell transplantation on renal ischemia-reperfusion injury. Forty rabbits were divided into four groups of 10 rabbits each. Thirty rabbits were used to establish the renal ischemia-reperfusion injury model, and ten rabbits served as the model group and were not treated. Among the 30 rabbits with renal ischemia-reperfusion injury, 10 rabbits were treated with induced peripheral blood mononuclear cells (PBMCs), and 10 other rabbits were treated with noninduced PBMCs. After three weekly treatments, the serum creatinine levels, urea nitrogen levels and urine protein concentrations were quantified. The kidneys were stained with hematoxylin-eosin (HE), periodic acid-Schiff (PAS) and Masson’s trichrome and then sent for commercial metabolomic testing. The kidneys of the rabbits in the model group showed different degrees of pathological changes, and the recovery of renal function was observed in the group treated with induced cells. The results indicate that PBMCs differentiate into multipotent stem cells after induction and exert a therapeutic effect on renal ischemia-reperfusion injury.
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spelling pubmed-77694662021-01-08 Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits Ruan, Guang-ping Yao, Xiang Lin, Qing-keng Li, Zi-an Cai, Xue-min Pang, Rong-qing Pan, Xing-hua PLoS One Research Article Ischemia-reperfusion injury is an important contributor to acute kidney injury and a major factor affecting early functional recovery after kidney transplantation. We conducted this experiment to investigate the protective effect of induced multipotent stem cell transplantation on renal ischemia-reperfusion injury. Forty rabbits were divided into four groups of 10 rabbits each. Thirty rabbits were used to establish the renal ischemia-reperfusion injury model, and ten rabbits served as the model group and were not treated. Among the 30 rabbits with renal ischemia-reperfusion injury, 10 rabbits were treated with induced peripheral blood mononuclear cells (PBMCs), and 10 other rabbits were treated with noninduced PBMCs. After three weekly treatments, the serum creatinine levels, urea nitrogen levels and urine protein concentrations were quantified. The kidneys were stained with hematoxylin-eosin (HE), periodic acid-Schiff (PAS) and Masson’s trichrome and then sent for commercial metabolomic testing. The kidneys of the rabbits in the model group showed different degrees of pathological changes, and the recovery of renal function was observed in the group treated with induced cells. The results indicate that PBMCs differentiate into multipotent stem cells after induction and exert a therapeutic effect on renal ischemia-reperfusion injury. Public Library of Science 2020-12-28 /pmc/articles/PMC7769466/ /pubmed/33370374 http://dx.doi.org/10.1371/journal.pone.0244160 Text en © 2020 Ruan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ruan, Guang-ping
Yao, Xiang
Lin, Qing-keng
Li, Zi-an
Cai, Xue-min
Pang, Rong-qing
Pan, Xing-hua
Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits
title Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits
title_full Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits
title_fullStr Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits
title_full_unstemmed Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits
title_short Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits
title_sort transplantation of chicken egg white extract-induced rabbit pbmcs as a treatment for renal ischemia-reperfusion injury in rabbits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769466/
https://www.ncbi.nlm.nih.gov/pubmed/33370374
http://dx.doi.org/10.1371/journal.pone.0244160
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