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The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
The tumour micro-environment (TME) plays a crucial role in the onset and progression of prostate cancer (PCa). Here we studied the potential of a selected panel of TME-markers to predict clinical recurrence (CLR) in PCa. Patient cohorts were matched for the presence or absence of CLR 5 years post-pr...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769484/ https://www.ncbi.nlm.nih.gov/pubmed/33370412 http://dx.doi.org/10.1371/journal.pone.0244663 |
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author | Gevaert, Thomas Van Eycke, Yves-Rémi Vanden Broeck, Thomas Van Poppel, Hein Salmon, Isabelle Rorive, Sandrine Muilwijk, Tim Claessens, Frank De Ridder, Dirk Joniau, Steven Decaestecker, Christine |
author_facet | Gevaert, Thomas Van Eycke, Yves-Rémi Vanden Broeck, Thomas Van Poppel, Hein Salmon, Isabelle Rorive, Sandrine Muilwijk, Tim Claessens, Frank De Ridder, Dirk Joniau, Steven Decaestecker, Christine |
author_sort | Gevaert, Thomas |
collection | PubMed |
description | The tumour micro-environment (TME) plays a crucial role in the onset and progression of prostate cancer (PCa). Here we studied the potential of a selected panel of TME-markers to predict clinical recurrence (CLR) in PCa. Patient cohorts were matched for the presence or absence of CLR 5 years post-prostatectomy. Tissue micro-arrays (TMA) were composed with both prostate non-tumour (PNT) and PCa tissue and subsequently processed for immunohistochemistry (IHC). The IHC panel included markers for cancer activated fibroblasts (CAFs), blood vessels and steroid hormone receptors ((SHR): androgen receptor (AR), progesterone receptor (PR) and estrogen receptor (ER)). Stained slides were digitalised, selectively annotated and analysed for percentage of marker expression with standardized and validated image analysis algorithms. A univariable analysis identified several TME markers with significant impact on CR: expression of CD31 (vascular marker) in PNT stroma, expression of alpha smooth muscle actin (αSMA) in PCa stroma, and PR expression ratio between PCa stroma and PNT stroma. A multivariable model, which included CD31 expression (vascular marker) in PNT stroma and PR expression ratio between PCa stroma and PNT stroma, could significantly stratify patients for CLR, with the identification of a low risk and high-risk subgroup. If validated and confirmed in an independent prospective series, this subgroup might have clinical potential for PCa patient stratification. |
format | Online Article Text |
id | pubmed-7769484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77694842021-01-08 The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients Gevaert, Thomas Van Eycke, Yves-Rémi Vanden Broeck, Thomas Van Poppel, Hein Salmon, Isabelle Rorive, Sandrine Muilwijk, Tim Claessens, Frank De Ridder, Dirk Joniau, Steven Decaestecker, Christine PLoS One Research Article The tumour micro-environment (TME) plays a crucial role in the onset and progression of prostate cancer (PCa). Here we studied the potential of a selected panel of TME-markers to predict clinical recurrence (CLR) in PCa. Patient cohorts were matched for the presence or absence of CLR 5 years post-prostatectomy. Tissue micro-arrays (TMA) were composed with both prostate non-tumour (PNT) and PCa tissue and subsequently processed for immunohistochemistry (IHC). The IHC panel included markers for cancer activated fibroblasts (CAFs), blood vessels and steroid hormone receptors ((SHR): androgen receptor (AR), progesterone receptor (PR) and estrogen receptor (ER)). Stained slides were digitalised, selectively annotated and analysed for percentage of marker expression with standardized and validated image analysis algorithms. A univariable analysis identified several TME markers with significant impact on CR: expression of CD31 (vascular marker) in PNT stroma, expression of alpha smooth muscle actin (αSMA) in PCa stroma, and PR expression ratio between PCa stroma and PNT stroma. A multivariable model, which included CD31 expression (vascular marker) in PNT stroma and PR expression ratio between PCa stroma and PNT stroma, could significantly stratify patients for CLR, with the identification of a low risk and high-risk subgroup. If validated and confirmed in an independent prospective series, this subgroup might have clinical potential for PCa patient stratification. Public Library of Science 2020-12-28 /pmc/articles/PMC7769484/ /pubmed/33370412 http://dx.doi.org/10.1371/journal.pone.0244663 Text en © 2020 Gevaert et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gevaert, Thomas Van Eycke, Yves-Rémi Vanden Broeck, Thomas Van Poppel, Hein Salmon, Isabelle Rorive, Sandrine Muilwijk, Tim Claessens, Frank De Ridder, Dirk Joniau, Steven Decaestecker, Christine The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients |
title | The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients |
title_full | The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients |
title_fullStr | The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients |
title_full_unstemmed | The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients |
title_short | The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients |
title_sort | potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769484/ https://www.ncbi.nlm.nih.gov/pubmed/33370412 http://dx.doi.org/10.1371/journal.pone.0244663 |
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