Cargando…

The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients

The tumour micro-environment (TME) plays a crucial role in the onset and progression of prostate cancer (PCa). Here we studied the potential of a selected panel of TME-markers to predict clinical recurrence (CLR) in PCa. Patient cohorts were matched for the presence or absence of CLR 5 years post-pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Gevaert, Thomas, Van Eycke, Yves-Rémi, Vanden Broeck, Thomas, Van Poppel, Hein, Salmon, Isabelle, Rorive, Sandrine, Muilwijk, Tim, Claessens, Frank, De Ridder, Dirk, Joniau, Steven, Decaestecker, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769484/
https://www.ncbi.nlm.nih.gov/pubmed/33370412
http://dx.doi.org/10.1371/journal.pone.0244663
_version_ 1783629336623972352
author Gevaert, Thomas
Van Eycke, Yves-Rémi
Vanden Broeck, Thomas
Van Poppel, Hein
Salmon, Isabelle
Rorive, Sandrine
Muilwijk, Tim
Claessens, Frank
De Ridder, Dirk
Joniau, Steven
Decaestecker, Christine
author_facet Gevaert, Thomas
Van Eycke, Yves-Rémi
Vanden Broeck, Thomas
Van Poppel, Hein
Salmon, Isabelle
Rorive, Sandrine
Muilwijk, Tim
Claessens, Frank
De Ridder, Dirk
Joniau, Steven
Decaestecker, Christine
author_sort Gevaert, Thomas
collection PubMed
description The tumour micro-environment (TME) plays a crucial role in the onset and progression of prostate cancer (PCa). Here we studied the potential of a selected panel of TME-markers to predict clinical recurrence (CLR) in PCa. Patient cohorts were matched for the presence or absence of CLR 5 years post-prostatectomy. Tissue micro-arrays (TMA) were composed with both prostate non-tumour (PNT) and PCa tissue and subsequently processed for immunohistochemistry (IHC). The IHC panel included markers for cancer activated fibroblasts (CAFs), blood vessels and steroid hormone receptors ((SHR): androgen receptor (AR), progesterone receptor (PR) and estrogen receptor (ER)). Stained slides were digitalised, selectively annotated and analysed for percentage of marker expression with standardized and validated image analysis algorithms. A univariable analysis identified several TME markers with significant impact on CR: expression of CD31 (vascular marker) in PNT stroma, expression of alpha smooth muscle actin (αSMA) in PCa stroma, and PR expression ratio between PCa stroma and PNT stroma. A multivariable model, which included CD31 expression (vascular marker) in PNT stroma and PR expression ratio between PCa stroma and PNT stroma, could significantly stratify patients for CLR, with the identification of a low risk and high-risk subgroup. If validated and confirmed in an independent prospective series, this subgroup might have clinical potential for PCa patient stratification.
format Online
Article
Text
id pubmed-7769484
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-77694842021-01-08 The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients Gevaert, Thomas Van Eycke, Yves-Rémi Vanden Broeck, Thomas Van Poppel, Hein Salmon, Isabelle Rorive, Sandrine Muilwijk, Tim Claessens, Frank De Ridder, Dirk Joniau, Steven Decaestecker, Christine PLoS One Research Article The tumour micro-environment (TME) plays a crucial role in the onset and progression of prostate cancer (PCa). Here we studied the potential of a selected panel of TME-markers to predict clinical recurrence (CLR) in PCa. Patient cohorts were matched for the presence or absence of CLR 5 years post-prostatectomy. Tissue micro-arrays (TMA) were composed with both prostate non-tumour (PNT) and PCa tissue and subsequently processed for immunohistochemistry (IHC). The IHC panel included markers for cancer activated fibroblasts (CAFs), blood vessels and steroid hormone receptors ((SHR): androgen receptor (AR), progesterone receptor (PR) and estrogen receptor (ER)). Stained slides were digitalised, selectively annotated and analysed for percentage of marker expression with standardized and validated image analysis algorithms. A univariable analysis identified several TME markers with significant impact on CR: expression of CD31 (vascular marker) in PNT stroma, expression of alpha smooth muscle actin (αSMA) in PCa stroma, and PR expression ratio between PCa stroma and PNT stroma. A multivariable model, which included CD31 expression (vascular marker) in PNT stroma and PR expression ratio between PCa stroma and PNT stroma, could significantly stratify patients for CLR, with the identification of a low risk and high-risk subgroup. If validated and confirmed in an independent prospective series, this subgroup might have clinical potential for PCa patient stratification. Public Library of Science 2020-12-28 /pmc/articles/PMC7769484/ /pubmed/33370412 http://dx.doi.org/10.1371/journal.pone.0244663 Text en © 2020 Gevaert et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gevaert, Thomas
Van Eycke, Yves-Rémi
Vanden Broeck, Thomas
Van Poppel, Hein
Salmon, Isabelle
Rorive, Sandrine
Muilwijk, Tim
Claessens, Frank
De Ridder, Dirk
Joniau, Steven
Decaestecker, Christine
The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
title The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
title_full The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
title_fullStr The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
title_full_unstemmed The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
title_short The potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
title_sort potential of tumour microenvironment markers to stratify the risk of recurrence in prostate cancer patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769484/
https://www.ncbi.nlm.nih.gov/pubmed/33370412
http://dx.doi.org/10.1371/journal.pone.0244663
work_keys_str_mv AT gevaertthomas thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT vaneyckeyvesremi thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT vandenbroeckthomas thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT vanpoppelhein thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT salmonisabelle thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT rorivesandrine thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT muilwijktim thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT claessensfrank thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT deridderdirk thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT joniausteven thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT decaesteckerchristine thepotentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT gevaertthomas potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT vaneyckeyvesremi potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT vandenbroeckthomas potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT vanpoppelhein potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT salmonisabelle potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT rorivesandrine potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT muilwijktim potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT claessensfrank potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT deridderdirk potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT joniausteven potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients
AT decaesteckerchristine potentialoftumourmicroenvironmentmarkerstostratifytheriskofrecurrenceinprostatecancerpatients