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Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice

The development of isoproterenol- (ISO-) induced oxidative stress in the myocardium results in myocardial necrosis. Sulforaphane (SFN-0.4% of sulforaphane from standardized broccoli sprout extract) possesses chemoprotective, antidiabetic, and antibacterial activities and is also active against cardi...

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Autores principales: Song, Lijuan, Srilakshmi, Mudduluri, Wu, Yi, Saleem, T. S. Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769644/
https://www.ncbi.nlm.nih.gov/pubmed/33415146
http://dx.doi.org/10.1155/2020/3610285
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author Song, Lijuan
Srilakshmi, Mudduluri
Wu, Yi
Saleem, T. S. Mohamed
author_facet Song, Lijuan
Srilakshmi, Mudduluri
Wu, Yi
Saleem, T. S. Mohamed
author_sort Song, Lijuan
collection PubMed
description The development of isoproterenol- (ISO-) induced oxidative stress in the myocardium results in myocardial necrosis. Sulforaphane (SFN-0.4% of sulforaphane from standardized broccoli sprout extract) possesses chemoprotective, antidiabetic, and antibacterial activities and is also active against cardiovascular-related problems due to its antioxidant properties. This study was designed to investigate the cardioprotective effect of SFN against isoproterenol-induced myocardial injury in mice. Healthy male Swiss albino mice weighing 20–30 g were used in this study. These mice were randomly divided into five groups (n = 6). All the mice in the experimental groups received isoproterenol (5 mg/kg bw, via i.p.) consecutively for 2 days. The mice were treated with SFN (4 mg/kg bw) and α-tocopherol (TCF) (10 mg/kg bw) by oral gavage for 1-7 days as pre- and posttreatment for the prophylactic and treatment groups, respectively. On day 10, the following parameters were studied: heart weight to body weight ratio, antioxidant parameters, and cardiac markers; and mitochondrial enzymes were estimated for cardioprotection. Administration of isoproterenol in mice showed an increased level of serum cardiac markers and heart mitochondrial ATPase enzymes. An increased level of myocardial thiobarbituric acid-reactive substance and decreased levels of endogenous antioxidant enzymes indicated that oxidative stress is induced by isoproterenol in the myocardium. The administration of SFN in mice restored the levels of all biochemical parameters to near-normal levels. Histopathological studies further confirmed the protective effect of sulforaphane. This study concluded that treatment with SFN boosts the endogenous antioxidant activity and prevents isoproterenol-induced myocardial injury.
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spelling pubmed-77696442021-01-06 Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice Song, Lijuan Srilakshmi, Mudduluri Wu, Yi Saleem, T. S. Mohamed Biomed Res Int Research Article The development of isoproterenol- (ISO-) induced oxidative stress in the myocardium results in myocardial necrosis. Sulforaphane (SFN-0.4% of sulforaphane from standardized broccoli sprout extract) possesses chemoprotective, antidiabetic, and antibacterial activities and is also active against cardiovascular-related problems due to its antioxidant properties. This study was designed to investigate the cardioprotective effect of SFN against isoproterenol-induced myocardial injury in mice. Healthy male Swiss albino mice weighing 20–30 g were used in this study. These mice were randomly divided into five groups (n = 6). All the mice in the experimental groups received isoproterenol (5 mg/kg bw, via i.p.) consecutively for 2 days. The mice were treated with SFN (4 mg/kg bw) and α-tocopherol (TCF) (10 mg/kg bw) by oral gavage for 1-7 days as pre- and posttreatment for the prophylactic and treatment groups, respectively. On day 10, the following parameters were studied: heart weight to body weight ratio, antioxidant parameters, and cardiac markers; and mitochondrial enzymes were estimated for cardioprotection. Administration of isoproterenol in mice showed an increased level of serum cardiac markers and heart mitochondrial ATPase enzymes. An increased level of myocardial thiobarbituric acid-reactive substance and decreased levels of endogenous antioxidant enzymes indicated that oxidative stress is induced by isoproterenol in the myocardium. The administration of SFN in mice restored the levels of all biochemical parameters to near-normal levels. Histopathological studies further confirmed the protective effect of sulforaphane. This study concluded that treatment with SFN boosts the endogenous antioxidant activity and prevents isoproterenol-induced myocardial injury. Hindawi 2020-12-19 /pmc/articles/PMC7769644/ /pubmed/33415146 http://dx.doi.org/10.1155/2020/3610285 Text en Copyright © 2020 Lijuan Song et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Song, Lijuan
Srilakshmi, Mudduluri
Wu, Yi
Saleem, T. S. Mohamed
Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice
title Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice
title_full Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice
title_fullStr Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice
title_full_unstemmed Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice
title_short Sulforaphane Attenuates Isoproterenol-Induced Myocardial Injury in Mice
title_sort sulforaphane attenuates isoproterenol-induced myocardial injury in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769644/
https://www.ncbi.nlm.nih.gov/pubmed/33415146
http://dx.doi.org/10.1155/2020/3610285
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