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gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells

Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data show...

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Autores principales: Yu, Heling, Xu, Hengyong, Yan, Chaoyang, Zhu, Shiliang, Lan, Xi, Lu, Yuxiang, He, Qijian, Yin, Huadong, Zhu, Qing, Zhao, Xiaoling, Li, Diyan, Liu, Yiping, Wang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769946/
https://www.ncbi.nlm.nih.gov/pubmed/33384993
http://dx.doi.org/10.3389/fcell.2020.587889
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author Yu, Heling
Xu, Hengyong
Yan, Chaoyang
Zhu, Shiliang
Lan, Xi
Lu, Yuxiang
He, Qijian
Yin, Huadong
Zhu, Qing
Zhao, Xiaoling
Li, Diyan
Liu, Yiping
Wang, Yan
author_facet Yu, Heling
Xu, Hengyong
Yan, Chaoyang
Zhu, Shiliang
Lan, Xi
Lu, Yuxiang
He, Qijian
Yin, Huadong
Zhu, Qing
Zhao, Xiaoling
Li, Diyan
Liu, Yiping
Wang, Yan
author_sort Yu, Heling
collection PubMed
description Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of gga-miR-148a-5p was significantly different in ALV-J-infected chicken spleens compared with non-infected chickens. The aim of this study was to investigate the functional roles of gga-miR-148a-5p and identify downstream targets regulated by gga-miR-148a-5p in ALV-J-infected chickens. We found that the expression of gga-miR-148a-5p was significantly downregulated during ALV-J infection of chicken embryo fibroblasts (CEF). Dual luciferase reporter assays demonstrated that PDPK1 is a direct target gene of gga-miR-148a-5p. In vitro, overexpression of gga-miR-148a-5p significantly promoted ALV-J-infected CEF cell proliferation, included cell cycle, whereas inhibition of gga-miR-148a-5p had an opposite effect. Inhibition of PDPK1 promoted the proliferation of ALV-J-infected cells but had no effect on the activity of NF-κB. Together, these results suggested that gga-miR-148a-5p targets PDPK1 to inhibit the proliferation and cell cycle of ALV-J-infected CEF cells. Our study provides a new understanding for the tumor mechanism of ALV-J infection.
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spelling pubmed-77699462020-12-30 gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells Yu, Heling Xu, Hengyong Yan, Chaoyang Zhu, Shiliang Lan, Xi Lu, Yuxiang He, Qijian Yin, Huadong Zhu, Qing Zhao, Xiaoling Li, Diyan Liu, Yiping Wang, Yan Front Cell Dev Biol Cell and Developmental Biology Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of gga-miR-148a-5p was significantly different in ALV-J-infected chicken spleens compared with non-infected chickens. The aim of this study was to investigate the functional roles of gga-miR-148a-5p and identify downstream targets regulated by gga-miR-148a-5p in ALV-J-infected chickens. We found that the expression of gga-miR-148a-5p was significantly downregulated during ALV-J infection of chicken embryo fibroblasts (CEF). Dual luciferase reporter assays demonstrated that PDPK1 is a direct target gene of gga-miR-148a-5p. In vitro, overexpression of gga-miR-148a-5p significantly promoted ALV-J-infected CEF cell proliferation, included cell cycle, whereas inhibition of gga-miR-148a-5p had an opposite effect. Inhibition of PDPK1 promoted the proliferation of ALV-J-infected cells but had no effect on the activity of NF-κB. Together, these results suggested that gga-miR-148a-5p targets PDPK1 to inhibit the proliferation and cell cycle of ALV-J-infected CEF cells. Our study provides a new understanding for the tumor mechanism of ALV-J infection. Frontiers Media S.A. 2020-12-15 /pmc/articles/PMC7769946/ /pubmed/33384993 http://dx.doi.org/10.3389/fcell.2020.587889 Text en Copyright © 2020 Yu, Xu, Yan, Zhu, Lan, Lu, He, Yin, Zhu, Zhao, Li, Liu and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Yu, Heling
Xu, Hengyong
Yan, Chaoyang
Zhu, Shiliang
Lan, Xi
Lu, Yuxiang
He, Qijian
Yin, Huadong
Zhu, Qing
Zhao, Xiaoling
Li, Diyan
Liu, Yiping
Wang, Yan
gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells
title gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells
title_full gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells
title_fullStr gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells
title_full_unstemmed gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells
title_short gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells
title_sort gga-mir-148a-5p-targeting pdpk1 inhibits proliferation and cell cycle progression of avain leukosis virus subgroup j (alv-j)-infected cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769946/
https://www.ncbi.nlm.nih.gov/pubmed/33384993
http://dx.doi.org/10.3389/fcell.2020.587889
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