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Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China

The midgut microbial community composition, structure, and function of field-collected mosquitoes may provide a way to exploit microbial function for mosquito-borne disease control. However, it is unclear how adult mosquitoes acquire their microbiome, how the microbiome affects life history traits a...

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Autores principales: Kang, Xun, Wang, Yanhong, Li, Siping, Sun, Xiaomei, Lu, Xiangyang, Rajaofera, Mamy Jayne Nelly, Lu, Yajun, Kang, Le, Zheng, Aihua, Zou, Zhen, Xia, Qianfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769952/
https://www.ncbi.nlm.nih.gov/pubmed/33384969
http://dx.doi.org/10.3389/fcimb.2020.596750
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author Kang, Xun
Wang, Yanhong
Li, Siping
Sun, Xiaomei
Lu, Xiangyang
Rajaofera, Mamy Jayne Nelly
Lu, Yajun
Kang, Le
Zheng, Aihua
Zou, Zhen
Xia, Qianfeng
author_facet Kang, Xun
Wang, Yanhong
Li, Siping
Sun, Xiaomei
Lu, Xiangyang
Rajaofera, Mamy Jayne Nelly
Lu, Yajun
Kang, Le
Zheng, Aihua
Zou, Zhen
Xia, Qianfeng
author_sort Kang, Xun
collection PubMed
description The midgut microbial community composition, structure, and function of field-collected mosquitoes may provide a way to exploit microbial function for mosquito-borne disease control. However, it is unclear how adult mosquitoes acquire their microbiome, how the microbiome affects life history traits and how the microbiome influences community structure. We analyzed the composition of 501 midgut bacterial communities from field-collected adult female mosquitoes, including Aedes albopictus, Aedes galloisi, Culex pallidothorax, Culex pipiens, Culex gelidus, and Armigeres subalbatus, across eight habitats using the HiSeq 4000 system and the V3−V4 hyper-variable region of 16S rRNA gene. After quality filtering and rarefaction, a total of 1421 operational taxonomic units, belonging to 29 phyla, 44 families, and 43 genera were identified. Proteobacteria (75.67%) were the most common phylum, followed by Firmicutes (10.38%), Bacteroidetes (6.87%), Thermi (4.60%), and Actinobacteria (1.58%). The genera Rickettsiaceae (33.00%), Enterobacteriaceae (20.27%), Enterococcaceae (7.49%), Aeromonadaceae (7.00%), Thermaceae (4.52%), and Moraxellaceae (4.31%) were dominant in the samples analyzed and accounted for 76.59% of the total genera. We characterized the midgut bacterial communities of six mosquito species in Hainan province, China. The gut bacterial communities were different in composition and abundance, among locations, for all mosquito species. There were significant differences in the gut microbial composition between some species and substantial variation in the gut microbiota between individuals of the same mosquito species. There was a marked variation in different mosquito gut microbiota within the same location. These results might be useful in the identification of microbial communities that could be exploited for disease control.
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spelling pubmed-77699522020-12-30 Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China Kang, Xun Wang, Yanhong Li, Siping Sun, Xiaomei Lu, Xiangyang Rajaofera, Mamy Jayne Nelly Lu, Yajun Kang, Le Zheng, Aihua Zou, Zhen Xia, Qianfeng Front Cell Infect Microbiol Cellular and Infection Microbiology The midgut microbial community composition, structure, and function of field-collected mosquitoes may provide a way to exploit microbial function for mosquito-borne disease control. However, it is unclear how adult mosquitoes acquire their microbiome, how the microbiome affects life history traits and how the microbiome influences community structure. We analyzed the composition of 501 midgut bacterial communities from field-collected adult female mosquitoes, including Aedes albopictus, Aedes galloisi, Culex pallidothorax, Culex pipiens, Culex gelidus, and Armigeres subalbatus, across eight habitats using the HiSeq 4000 system and the V3−V4 hyper-variable region of 16S rRNA gene. After quality filtering and rarefaction, a total of 1421 operational taxonomic units, belonging to 29 phyla, 44 families, and 43 genera were identified. Proteobacteria (75.67%) were the most common phylum, followed by Firmicutes (10.38%), Bacteroidetes (6.87%), Thermi (4.60%), and Actinobacteria (1.58%). The genera Rickettsiaceae (33.00%), Enterobacteriaceae (20.27%), Enterococcaceae (7.49%), Aeromonadaceae (7.00%), Thermaceae (4.52%), and Moraxellaceae (4.31%) were dominant in the samples analyzed and accounted for 76.59% of the total genera. We characterized the midgut bacterial communities of six mosquito species in Hainan province, China. The gut bacterial communities were different in composition and abundance, among locations, for all mosquito species. There were significant differences in the gut microbial composition between some species and substantial variation in the gut microbiota between individuals of the same mosquito species. There was a marked variation in different mosquito gut microbiota within the same location. These results might be useful in the identification of microbial communities that could be exploited for disease control. Frontiers Media S.A. 2020-12-15 /pmc/articles/PMC7769952/ /pubmed/33384969 http://dx.doi.org/10.3389/fcimb.2020.596750 Text en Copyright © 2020 Kang, Wang, Li, Sun, Lu, Rajaofera, Lu, Kang, Zheng, Zou and Xia http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Kang, Xun
Wang, Yanhong
Li, Siping
Sun, Xiaomei
Lu, Xiangyang
Rajaofera, Mamy Jayne Nelly
Lu, Yajun
Kang, Le
Zheng, Aihua
Zou, Zhen
Xia, Qianfeng
Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China
title Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China
title_full Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China
title_fullStr Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China
title_full_unstemmed Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China
title_short Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China
title_sort comparative analysis of the gut microbiota of adult mosquitoes from eight locations in hainan, china
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769952/
https://www.ncbi.nlm.nih.gov/pubmed/33384969
http://dx.doi.org/10.3389/fcimb.2020.596750
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