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Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model

This study compares the mechanical response of the commonly used annulus fibrosus (AF) puncture injury model of the intervertebral disc (IVD) and a newly proposed AF failure at the endplate junction (delamination) on ex vivo bovine IVDs. Biocompatibility and mechanics of a newly developed repair str...

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Autores principales: Alexeev, Dmitriy, Cui, Shangbin, Grad, Sibylle, Li, Zhen, Ferguson, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770194/
https://www.ncbi.nlm.nih.gov/pubmed/33392447
http://dx.doi.org/10.1002/jsp2.1107
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author Alexeev, Dmitriy
Cui, Shangbin
Grad, Sibylle
Li, Zhen
Ferguson, Stephen J.
author_facet Alexeev, Dmitriy
Cui, Shangbin
Grad, Sibylle
Li, Zhen
Ferguson, Stephen J.
author_sort Alexeev, Dmitriy
collection PubMed
description This study compares the mechanical response of the commonly used annulus fibrosus (AF) puncture injury model of the intervertebral disc (IVD) and a newly proposed AF failure at the endplate junction (delamination) on ex vivo bovine IVDs. Biocompatibility and mechanics of a newly developed repair strategy comprising of electrospun polycaprolactone (PCL) scaffold and fibrin‐genipin (FibGen) adhesive was tested on the delamination model. The study found no significant difference in the mechanical response to compressive loading between the two models. Primary goals of the repair strategy to create a tight seal on the damage area and restore mechanical properties, while showing minimal cytotoxicity, were broadly achieved. Postrepair, the IVDs showed a significant restoration of mechanical properties compared to the injured samples for the delamination model. The FibGen glue showed a limited toxicity in the AF and produced a resilient and mechanically stable seal on the damaged area.
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spelling pubmed-77701942020-12-31 Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model Alexeev, Dmitriy Cui, Shangbin Grad, Sibylle Li, Zhen Ferguson, Stephen J. JOR Spine Research Articles This study compares the mechanical response of the commonly used annulus fibrosus (AF) puncture injury model of the intervertebral disc (IVD) and a newly proposed AF failure at the endplate junction (delamination) on ex vivo bovine IVDs. Biocompatibility and mechanics of a newly developed repair strategy comprising of electrospun polycaprolactone (PCL) scaffold and fibrin‐genipin (FibGen) adhesive was tested on the delamination model. The study found no significant difference in the mechanical response to compressive loading between the two models. Primary goals of the repair strategy to create a tight seal on the damage area and restore mechanical properties, while showing minimal cytotoxicity, were broadly achieved. Postrepair, the IVDs showed a significant restoration of mechanical properties compared to the injured samples for the delamination model. The FibGen glue showed a limited toxicity in the AF and produced a resilient and mechanically stable seal on the damaged area. John Wiley & Sons, Inc. 2020-07-16 /pmc/articles/PMC7770194/ /pubmed/33392447 http://dx.doi.org/10.1002/jsp2.1107 Text en © 2020 The Authors. JOR Spine published by Wiley Periodicals LLC. on behalf of Orthopaedic Research Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Alexeev, Dmitriy
Cui, Shangbin
Grad, Sibylle
Li, Zhen
Ferguson, Stephen J.
Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model
title Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model
title_full Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model
title_fullStr Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model
title_full_unstemmed Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model
title_short Mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model
title_sort mechanical and biological characterization of a composite annulus fibrosus repair strategy in an endplate delamination model
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770194/
https://www.ncbi.nlm.nih.gov/pubmed/33392447
http://dx.doi.org/10.1002/jsp2.1107
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