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Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice

Salmonella enterica serovar Typhimurium (S). Typhimurium is a primary foodborne pathogen infecting both humans and animals. Salmonella plasmid virulence C (spvC) gene is closely related to S. Typhimurium dissemination in mice, while the mechanisms remain to be fully elucidated. Pyroptosis, a gasderm...

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Autores principales: Zuo, Lingli, Zhou, Liting, Wu, Chaoyi, Wang, Yanlin, Li, Yuanyuan, Huang, Rui, Wu, Shuyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770238/
https://www.ncbi.nlm.nih.gov/pubmed/33384666
http://dx.doi.org/10.3389/fmicb.2020.562491
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author Zuo, Lingli
Zhou, Liting
Wu, Chaoyi
Wang, Yanlin
Li, Yuanyuan
Huang, Rui
Wu, Shuyan
author_facet Zuo, Lingli
Zhou, Liting
Wu, Chaoyi
Wang, Yanlin
Li, Yuanyuan
Huang, Rui
Wu, Shuyan
author_sort Zuo, Lingli
collection PubMed
description Salmonella enterica serovar Typhimurium (S). Typhimurium is a primary foodborne pathogen infecting both humans and animals. Salmonella plasmid virulence C (spvC) gene is closely related to S. Typhimurium dissemination in mice, while the mechanisms remain to be fully elucidated. Pyroptosis, a gasdermin-mediated inflammatory cell death, plays a role in host defense against bacterial infection, whereas the effect of spvC on pyroptosis and its function in inflammatory injury induced by S. Typhimurium are rather limited. In our study, C57BL/6 mice and J774A.1 cells infected with S. Typhimurium wild-type strain SL1344, spvC deletion mutant, spvC K136A site-directed mutant, and complemented strain were used to investigate potential pathogenesis of spvC. We verity that SpvC attenuates intestinal inflammation, suppresses pyroptosis through phosphothreonine lyase activity, and reduces pyroptosis in the ceca. Moreover, the reduction of inflammation via spvC results in systemic infection. These findings demonstrate that spvC inhibits pyroptosis and intestinal inflammation to promote bacterial dissemination, which provide new strategies for controlling systemic infection caused by Salmonella and novel insights for the treatment of other corresponding diseases.
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spelling pubmed-77702382020-12-30 Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice Zuo, Lingli Zhou, Liting Wu, Chaoyi Wang, Yanlin Li, Yuanyuan Huang, Rui Wu, Shuyan Front Microbiol Microbiology Salmonella enterica serovar Typhimurium (S). Typhimurium is a primary foodborne pathogen infecting both humans and animals. Salmonella plasmid virulence C (spvC) gene is closely related to S. Typhimurium dissemination in mice, while the mechanisms remain to be fully elucidated. Pyroptosis, a gasdermin-mediated inflammatory cell death, plays a role in host defense against bacterial infection, whereas the effect of spvC on pyroptosis and its function in inflammatory injury induced by S. Typhimurium are rather limited. In our study, C57BL/6 mice and J774A.1 cells infected with S. Typhimurium wild-type strain SL1344, spvC deletion mutant, spvC K136A site-directed mutant, and complemented strain were used to investigate potential pathogenesis of spvC. We verity that SpvC attenuates intestinal inflammation, suppresses pyroptosis through phosphothreonine lyase activity, and reduces pyroptosis in the ceca. Moreover, the reduction of inflammation via spvC results in systemic infection. These findings demonstrate that spvC inhibits pyroptosis and intestinal inflammation to promote bacterial dissemination, which provide new strategies for controlling systemic infection caused by Salmonella and novel insights for the treatment of other corresponding diseases. Frontiers Media S.A. 2020-12-15 /pmc/articles/PMC7770238/ /pubmed/33384666 http://dx.doi.org/10.3389/fmicb.2020.562491 Text en Copyright © 2020 Zuo, Zhou, Wu, Wang, Li, Huang and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zuo, Lingli
Zhou, Liting
Wu, Chaoyi
Wang, Yanlin
Li, Yuanyuan
Huang, Rui
Wu, Shuyan
Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice
title Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice
title_full Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice
title_fullStr Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice
title_full_unstemmed Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice
title_short Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice
title_sort salmonella spvc gene inhibits pyroptosis and intestinal inflammation to aggravate systemic infection in mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770238/
https://www.ncbi.nlm.nih.gov/pubmed/33384666
http://dx.doi.org/10.3389/fmicb.2020.562491
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