Effect of incorporation of triphala into AH26 sealer on its cytotoxicity at different intervals

Background. One of the essential properties of sealers used during endodontic treatment is their biocompatibility. Different materials are added to these sealers to improve their properties, including antibacterial activity. In recent years, there has been an increase in interest in the use of herbal medicines. This study aimed to evaluate the effect of incorporating triphala into AH26 sealer on its cytotoxicity on gingival fibroblasts at different intervals after mixing. Methods. In the present in vitro study, the cytotoxicity of AH26 sealer was evaluated once in its pure form and once after mixing it with triphala at 24-, 48-, and 72-hour, and 7-day intervals after mixing using the standard MTT assay protocol on gingival fibroblasts. Results. Two-way ANOVA was used to evaluate the effect of groups on the mean changes in cytotoxicity at different time intervals at a significance level of P<0.05. The results showed that the incorporation of triphala into the AH26 sealer did not increase or decrease its cytotoxicity (P=0.909). Besides, there was a decrease in cytotoxicity in both study groups. However, there was a relative increase in the sealers’ cytotoxicity in both groups in the first 72 hours (P<0.0001). Conclusion. Considering the well-established antibacterial properties of triphala in our previous study, the present study’s results showed that the incorporation of triphala into the AH26 sealer did not increase the cytotoxicity of the sealer. Therefore, it can be incorporated into the AH26 sealer to improve the other properties of the sealer, including its antibacterial activity.