Relationship between expression mRNA gene Treg, Treg, CD4(+), and CD8(+) protein levels with TST in tuberculosis children: A nested case-control

BACKGROUND: The ability of Mycobacterium tuberculosis to survive intracellularly, provides a cellular adaptive immune response played by specific T cells to defend against tuberculosis. The adaptive immune response to Bacillus of Calmette and Guerin (BCG) immunization is responded to by B cells, T Follicular B helper, T regulatory, restriction CD1, CD8(+), CD4(+), Th1, Th2, and Th17. BCG immunization can cause a tuberculin test reaction to being positive. The tuberculin test is a method for diagnosing TB infection and for screening individuals for latent infection and assessing the rate of TB infection in a given population. METHODS: a nested case-control survey was conducted on patients with a diagnosis of TB and parents 0–18 years of age from 3 hospitals in Indonesia during September–November 2019 with a total sample of 69 people undergoing clinical examinations, supporting and diagnosing subjects, blood sampling 1–2 cc for examination mRNA gene Treg, Treg, CD 4+, and CD 8+, then centrifuged at 3000 rpm for 10 min to support blood cells and serum. RESULTS: There was a significant relationship between expression of mRNA gene Treg with TST (p = 0,000), Treg with TST (p = 0,000), and CD4(+) with TST (p = 0,000). Meanwhile, CD8 + was not significantly associated with TST (p = 0.118). CONCLUSIONS: It is necessary to check the expression of mRNA gene Treg, Treg, CD4(+), and CD8(+) with more samples to find the mean value that shows the protective value of further TB.