Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus

We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay...

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Detalles Bibliográficos
Autores principales: Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7771451/
https://www.ncbi.nlm.nih.gov/pubmed/33087429
http://dx.doi.org/10.1128/JCM.01764-20
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author Velusamy, Srinivasan
Jordak, Katherine
Kupor, Madeline
Chochua, Sopio
McGee, Lesley
Beall, Bernard
author_facet Velusamy, Srinivasan
Jordak, Katherine
Kupor, Madeline
Chochua, Sopio
McGee, Lesley
Beall, Bernard
author_sort Velusamy, Srinivasan
collection PubMed
description We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations.
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spelling pubmed-77714512021-06-17 Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus Velusamy, Srinivasan Jordak, Katherine Kupor, Madeline Chochua, Sopio McGee, Lesley Beall, Bernard J Clin Microbiol Bacteriology We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations. American Society for Microbiology 2020-12-17 /pmc/articles/PMC7771451/ /pubmed/33087429 http://dx.doi.org/10.1128/JCM.01764-20 Text en Copyright © 2020 Velusamy et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bacteriology
Velusamy, Srinivasan
Jordak, Katherine
Kupor, Madeline
Chochua, Sopio
McGee, Lesley
Beall, Bernard
Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
title Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
title_full Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
title_fullStr Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
title_full_unstemmed Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
title_short Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
title_sort sequential quadriplex real-time pcr for identifying 20 common emm types of group a streptococcus
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7771451/
https://www.ncbi.nlm.nih.gov/pubmed/33087429
http://dx.doi.org/10.1128/JCM.01764-20
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