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Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo
Pinkwater Biosolve (BioSolve®) is one bioremediating chemical which has been widely used for cleanup of crude oil spill in Nigeria. It is a water-based formulation of nonionic surfactants and other specialty chemicals. The level of toxicity resulting from environmental exposure to this chemical has...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772439/ https://www.ncbi.nlm.nih.gov/pubmed/33384940 http://dx.doi.org/10.1016/j.toxrep.2020.04.009 |
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author | Edet, Francis Olubodun, Stella Uansoje, Sylvester Rotimi, Solomon Eriyamremu, George |
author_facet | Edet, Francis Olubodun, Stella Uansoje, Sylvester Rotimi, Solomon Eriyamremu, George |
author_sort | Edet, Francis |
collection | PubMed |
description | Pinkwater Biosolve (BioSolve®) is one bioremediating chemical which has been widely used for cleanup of crude oil spill in Nigeria. It is a water-based formulation of nonionic surfactants and other specialty chemicals. The level of toxicity resulting from environmental exposure to this chemical has not been well understood. The level of expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo were investigated. The embryo were pretreated with different doses of BioSolve, soil water from remediated soil sample, 10% soluble crude oil portion and a combination of the BioSolve with the soluble crude portion all constituted in normal saline solution. Reverse transcriptase PCR technique was used to assess the expression of hepatic proinflammatory cytokines. Histological examination was also carried out on liver fragments. The results showed that the pretreatment caused lesion on hepatocytes of all tested chick embryos except in the group administered with normal saline solution when compared with the normal control. The chick embryo exposed to 0.5 mg/kg BioSolve, 5% decanted soil water (v/v) obtained from crude oil remediated (using BioSolve) soil, and 10% (v/v) decanted crude oil remediated (using BioSolve) soil water all showed significant expression (at p < 0.05) of IFNγ, TGFβ1, IL-1β, IL-2 and TNF. The group treated with 10% soluble portion of crude oil showed significant changes in their expression pattern when compared with the control; TNF was up regulated, while IL-1β, IFNγ and TGFβ1 were down regulated. Only TNF was upregulated at p < 0.05 indicating the chances of soluble portion of crude oil causing cancer. IFNγ, TGFβ1, IL-1β and IL-2 were all down regulated significantly at p < 0.05 due to exposure to a combination of 10% soluble crude and 0.036 mg/kg BioSolve. The combination of 10% soluble crude and 0.36 mg/kg BioSolve caused lethal effect to the developing chick embryo. |
format | Online Article Text |
id | pubmed-7772439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77724392020-12-30 Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo Edet, Francis Olubodun, Stella Uansoje, Sylvester Rotimi, Solomon Eriyamremu, George Toxicol Rep Recent trends in environmental toxicology and sustainable agriculture Pinkwater Biosolve (BioSolve®) is one bioremediating chemical which has been widely used for cleanup of crude oil spill in Nigeria. It is a water-based formulation of nonionic surfactants and other specialty chemicals. The level of toxicity resulting from environmental exposure to this chemical has not been well understood. The level of expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo were investigated. The embryo were pretreated with different doses of BioSolve, soil water from remediated soil sample, 10% soluble crude oil portion and a combination of the BioSolve with the soluble crude portion all constituted in normal saline solution. Reverse transcriptase PCR technique was used to assess the expression of hepatic proinflammatory cytokines. Histological examination was also carried out on liver fragments. The results showed that the pretreatment caused lesion on hepatocytes of all tested chick embryos except in the group administered with normal saline solution when compared with the normal control. The chick embryo exposed to 0.5 mg/kg BioSolve, 5% decanted soil water (v/v) obtained from crude oil remediated (using BioSolve) soil, and 10% (v/v) decanted crude oil remediated (using BioSolve) soil water all showed significant expression (at p < 0.05) of IFNγ, TGFβ1, IL-1β, IL-2 and TNF. The group treated with 10% soluble portion of crude oil showed significant changes in their expression pattern when compared with the control; TNF was up regulated, while IL-1β, IFNγ and TGFβ1 were down regulated. Only TNF was upregulated at p < 0.05 indicating the chances of soluble portion of crude oil causing cancer. IFNγ, TGFβ1, IL-1β and IL-2 were all down regulated significantly at p < 0.05 due to exposure to a combination of 10% soluble crude and 0.036 mg/kg BioSolve. The combination of 10% soluble crude and 0.36 mg/kg BioSolve caused lethal effect to the developing chick embryo. Elsevier 2020-04-22 /pmc/articles/PMC7772439/ /pubmed/33384940 http://dx.doi.org/10.1016/j.toxrep.2020.04.009 Text en © 2020 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Recent trends in environmental toxicology and sustainable agriculture Edet, Francis Olubodun, Stella Uansoje, Sylvester Rotimi, Solomon Eriyamremu, George Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo |
title | Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo |
title_full | Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo |
title_fullStr | Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo |
title_full_unstemmed | Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo |
title_short | Effect of Pinkwater BioSolve on expression of proinflammatory cytokines and histological changes in Gallus domesticus embryo |
title_sort | effect of pinkwater biosolve on expression of proinflammatory cytokines and histological changes in gallus domesticus embryo |
topic | Recent trends in environmental toxicology and sustainable agriculture |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772439/ https://www.ncbi.nlm.nih.gov/pubmed/33384940 http://dx.doi.org/10.1016/j.toxrep.2020.04.009 |
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