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Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet
BACKGROUND: Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). OBJECTIVE: The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytoki...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772498/ https://www.ncbi.nlm.nih.gov/pubmed/32798194 http://dx.doi.org/10.1016/j.jaim.2020.06.009 |
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author | Lestari, Sri Rahayu Atho’illah, Mochammad Fitri Christina, Yuyun Ika Rifa’i, Muhaimin |
author_facet | Lestari, Sri Rahayu Atho’illah, Mochammad Fitri Christina, Yuyun Ika Rifa’i, Muhaimin |
author_sort | Lestari, Sri Rahayu |
collection | PubMed |
description | BACKGROUND: Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). OBJECTIVE: The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytokine expression in HFD mice. METHODS: Mice were divided into six groups: ND (normal diet); HFD (high-fat diet without treatment); HFD + Simv (HFD + simvastatin 2.6 mg/kg body weight); and HFD + SGO 1–3 (high-fat diet + single garlic oil in a dose of 12.5, 25, and 50 mg/kg body weight), respectively. Treatments were orally given every day for 45 days. At the end of treatments, lymphocytes were isolated from mice spleen. The relative number of T-cells and proinflammatory cytokines were measured using flow-cytometry and analyzed using one-way ANOVA (p < 0.05). RESULT: Our result indicated that HFD mice had lower naive T cells (CD4+CD62L+) than normal mice (p < 0.05). SGO treatment in HFD mice increased the relative number of naïve T cells. HFD treatment increased the expression of TNF-α and IFN-γ through NF-κB expression. Furthermore, SGO treatment improved the expression of TNF-α and IFN-γ. CONCLUSION: Our study suggests that SGO could act as a promising prospect for therapy to improve chronic inflammation in a HFD. |
format | Online Article Text |
id | pubmed-7772498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77724982020-12-30 Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet Lestari, Sri Rahayu Atho’illah, Mochammad Fitri Christina, Yuyun Ika Rifa’i, Muhaimin J Ayurveda Integr Med Original Research Article (Experimental) BACKGROUND: Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). OBJECTIVE: The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytokine expression in HFD mice. METHODS: Mice were divided into six groups: ND (normal diet); HFD (high-fat diet without treatment); HFD + Simv (HFD + simvastatin 2.6 mg/kg body weight); and HFD + SGO 1–3 (high-fat diet + single garlic oil in a dose of 12.5, 25, and 50 mg/kg body weight), respectively. Treatments were orally given every day for 45 days. At the end of treatments, lymphocytes were isolated from mice spleen. The relative number of T-cells and proinflammatory cytokines were measured using flow-cytometry and analyzed using one-way ANOVA (p < 0.05). RESULT: Our result indicated that HFD mice had lower naive T cells (CD4+CD62L+) than normal mice (p < 0.05). SGO treatment in HFD mice increased the relative number of naïve T cells. HFD treatment increased the expression of TNF-α and IFN-γ through NF-κB expression. Furthermore, SGO treatment improved the expression of TNF-α and IFN-γ. CONCLUSION: Our study suggests that SGO could act as a promising prospect for therapy to improve chronic inflammation in a HFD. Elsevier 2020 2020-08-12 /pmc/articles/PMC7772498/ /pubmed/32798194 http://dx.doi.org/10.1016/j.jaim.2020.06.009 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Article (Experimental) Lestari, Sri Rahayu Atho’illah, Mochammad Fitri Christina, Yuyun Ika Rifa’i, Muhaimin Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
title | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
title_full | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
title_fullStr | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
title_full_unstemmed | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
title_short | Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet |
title_sort | single garlic oil modulates t cells activation and proinflammatory cytokine in mice with high fat diet |
topic | Original Research Article (Experimental) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772498/ https://www.ncbi.nlm.nih.gov/pubmed/32798194 http://dx.doi.org/10.1016/j.jaim.2020.06.009 |
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