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Mapping germ-layer specification preventing genes in hPSCs via genome-scale CRISPR screening

Understanding the biological processes that determine the entry of three germ layers of human pluripotent stem cells (hPSCs) is a central question in developmental and stem cell biology. Here, we genetically engineered hPSCs with the germ layer reporter and inducible CRISPR/Cas9 knockout system, and...

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Detalles Bibliográficos
Autores principales:	Xu, Xiangjie, Du, Yanhua, Ma, Lin, Zhang, Shuwei, Shi, Lei, Chen, Zhenyu, Zhou, Zhongshu, Hui, Yi, Liu, Yang, Fang, Yujiang, Fan, Beibei, Liu, Zhongliang, Li, Nan, Zhou, Shanshan, Jiang, Cizhong, Liu, Ling, Zhang, Xiaoqing
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772566/ https://www.ncbi.nlm.nih.gov/pubmed/33385119 http://dx.doi.org/10.1016/j.isci.2020.101926

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