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Experimental determination of the bioluminescence resonance energy transfer (BRET) Förster distances of NanoBRET and red-shifted BRET pairs

Bioluminescence Resonance Energy Transfer (BRET) is widely applied to study protein-protein interactions, as well as increasingly to monitor both ligand binding and molecular rearrangements. The Förster distance (R(0)) describes the physical distance between the two chromophores at which 50% of the...

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Detalles Bibliográficos
Autores principales: Weihs, Felix, Wang, Jian, Pfleger, Kevin D.G., Dacres, Helen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772631/
https://www.ncbi.nlm.nih.gov/pubmed/33392495
http://dx.doi.org/10.1016/j.acax.2020.100059