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Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca

As the demand for alternatives to antibiotic growth promoters (AGP) increases in food animal production, phytobiotic compounds gain popularity because of their ability to mimic the desirable bioactive properties of AGP. Chestnut tannins (ChT) are one of many phytobiotic compounds used as feed additi...

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Autores principales: Lee, Annah, Dal Pont, Gabriela Cardoso, Farnell, Morgan B., Jarvis, Stephanie, Battaglia, Michele, Arsenault, Ryan J., Kogut, Michael H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772675/
https://www.ncbi.nlm.nih.gov/pubmed/33357706
http://dx.doi.org/10.1016/j.psj.2020.09.085
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author Lee, Annah
Dal Pont, Gabriela Cardoso
Farnell, Morgan B.
Jarvis, Stephanie
Battaglia, Michele
Arsenault, Ryan J.
Kogut, Michael H.
author_facet Lee, Annah
Dal Pont, Gabriela Cardoso
Farnell, Morgan B.
Jarvis, Stephanie
Battaglia, Michele
Arsenault, Ryan J.
Kogut, Michael H.
author_sort Lee, Annah
collection PubMed
description As the demand for alternatives to antibiotic growth promoters (AGP) increases in food animal production, phytobiotic compounds gain popularity because of their ability to mimic the desirable bioactive properties of AGP. Chestnut tannins (ChT) are one of many phytobiotic compounds used as feed additives, particularly in South America, for broilers because of its favorable antimicrobial and growth promotion capabilities. Although studies have observed the microbiological and immunologic effects of ChT, there is a lack of studies evaluating the metabolic function of ChT. Therefore, the objective of this study was to characterize the cecal metabolic changes induced by ChT inclusion and how they relate to growth promotion. A total of 200 day-of-hatch broiler chicks were separated into 2 feed treatment groups: control and 1% ChT. The ceca from all the chicks in the treatment groups were collected on day 2, 4, 6, 8, and 10 after hatch. The cytokine mRNA quantitative RT-PCR was determined using TaqMan gene expression assays for IL-1B, IL-6, IL-8, IL-10, and interferon gamma quantification. The cytokine expression showed highly significant increased expressions of IL-6 and IL-10 on day 2 and 6, whereas the other proinflammatory cytokines did not have significantly increased expression. The results from the kinome array demonstrated that the ceca from birds fed with 1% ChT had significant (P < 0.05) metabolic alterations based on the number of peptides when compared with the control group across all day tested. The increased expression of IL-6 appeared to be strongly indicative of altered metabolism, whereas the increased expression of IL-10 indicated the regulatory effect against other proinflammatory cytokines other than IL-6. The ChT initiate a metabolic mechanism during the first 10 d in the broiler. For the first time, we show that a phytobiotic product initially modulates metabolism while also potentially supporting growth and feed efficiency downstream. In conclusion, a metabolic phenotype alteration in the ceca of chickens fed ChT may indicate the importance of enhanced broiler gut health.
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spelling pubmed-77726752020-12-31 Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca Lee, Annah Dal Pont, Gabriela Cardoso Farnell, Morgan B. Jarvis, Stephanie Battaglia, Michele Arsenault, Ryan J. Kogut, Michael H. Poult Sci Immunology, Health and Disease As the demand for alternatives to antibiotic growth promoters (AGP) increases in food animal production, phytobiotic compounds gain popularity because of their ability to mimic the desirable bioactive properties of AGP. Chestnut tannins (ChT) are one of many phytobiotic compounds used as feed additives, particularly in South America, for broilers because of its favorable antimicrobial and growth promotion capabilities. Although studies have observed the microbiological and immunologic effects of ChT, there is a lack of studies evaluating the metabolic function of ChT. Therefore, the objective of this study was to characterize the cecal metabolic changes induced by ChT inclusion and how they relate to growth promotion. A total of 200 day-of-hatch broiler chicks were separated into 2 feed treatment groups: control and 1% ChT. The ceca from all the chicks in the treatment groups were collected on day 2, 4, 6, 8, and 10 after hatch. The cytokine mRNA quantitative RT-PCR was determined using TaqMan gene expression assays for IL-1B, IL-6, IL-8, IL-10, and interferon gamma quantification. The cytokine expression showed highly significant increased expressions of IL-6 and IL-10 on day 2 and 6, whereas the other proinflammatory cytokines did not have significantly increased expression. The results from the kinome array demonstrated that the ceca from birds fed with 1% ChT had significant (P < 0.05) metabolic alterations based on the number of peptides when compared with the control group across all day tested. The increased expression of IL-6 appeared to be strongly indicative of altered metabolism, whereas the increased expression of IL-10 indicated the regulatory effect against other proinflammatory cytokines other than IL-6. The ChT initiate a metabolic mechanism during the first 10 d in the broiler. For the first time, we show that a phytobiotic product initially modulates metabolism while also potentially supporting growth and feed efficiency downstream. In conclusion, a metabolic phenotype alteration in the ceca of chickens fed ChT may indicate the importance of enhanced broiler gut health. Elsevier 2020-10-08 /pmc/articles/PMC7772675/ /pubmed/33357706 http://dx.doi.org/10.1016/j.psj.2020.09.085 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Immunology, Health and Disease
Lee, Annah
Dal Pont, Gabriela Cardoso
Farnell, Morgan B.
Jarvis, Stephanie
Battaglia, Michele
Arsenault, Ryan J.
Kogut, Michael H.
Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca
title Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca
title_full Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca
title_fullStr Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca
title_full_unstemmed Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca
title_short Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca
title_sort supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca
topic Immunology, Health and Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772675/
https://www.ncbi.nlm.nih.gov/pubmed/33357706
http://dx.doi.org/10.1016/j.psj.2020.09.085
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