Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana

Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named P(PSP1)-BnPSP-1) and analyzed the function of its truncated fragments in A...

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Autores principales: Wang, Yunhe, Bao, Yaning, Zheng, Yancheng, Guo, Ping’an, Peng, Dingxiang, Wang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772962/
https://www.ncbi.nlm.nih.gov/pubmed/33391335
http://dx.doi.org/10.3389/fgene.2020.553265
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author Wang, Yunhe
Bao, Yaning
Zheng, Yancheng
Guo, Ping’an
Peng, Dingxiang
Wang, Bo
author_facet Wang, Yunhe
Bao, Yaning
Zheng, Yancheng
Guo, Ping’an
Peng, Dingxiang
Wang, Bo
author_sort Wang, Yunhe
collection PubMed
description Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named P(PSP1)-BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that P(PSP1)-BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying P(PSP1)-BnPSP-1 and its truncated fragments, only P(PSP1–5)-BnPSP-1 (−600 to −1 bp region of P(PSP1)-BnPSP-1) is stably expressed and exhibits phloem specificity. Our findings suggest that P(PSP1–5)-BnPSP-1 can be used as a phloem specific promoter for further research.
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spelling pubmed-77729622020-12-31 Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana Wang, Yunhe Bao, Yaning Zheng, Yancheng Guo, Ping’an Peng, Dingxiang Wang, Bo Front Genet Genetics Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named P(PSP1)-BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that P(PSP1)-BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying P(PSP1)-BnPSP-1 and its truncated fragments, only P(PSP1–5)-BnPSP-1 (−600 to −1 bp region of P(PSP1)-BnPSP-1) is stably expressed and exhibits phloem specificity. Our findings suggest that P(PSP1–5)-BnPSP-1 can be used as a phloem specific promoter for further research. Frontiers Media S.A. 2020-12-16 /pmc/articles/PMC7772962/ /pubmed/33391335 http://dx.doi.org/10.3389/fgene.2020.553265 Text en Copyright © 2020 Wang, Bao, Zheng, Guo, Peng and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Yunhe
Bao, Yaning
Zheng, Yancheng
Guo, Ping’an
Peng, Dingxiang
Wang, Bo
Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana
title Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana
title_full Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana
title_fullStr Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana
title_full_unstemmed Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana
title_short Promoter P(PSP1–5)-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana
title_sort promoter p(psp1–5)-bnpsp-1 from ramie (boehmeria nivea l. gaud.) can drive phloem-specific gus expression in arabidopsis thaliana
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772962/
https://www.ncbi.nlm.nih.gov/pubmed/33391335
http://dx.doi.org/10.3389/fgene.2020.553265
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