Cargando…

Stability of SARS-CoV-2 RNA in Viral Lysis Buffer Stored at Different Temperatures

Objectives The present COVID-19 pandemic resulted in an increased need for molecular diagnostic testing. Delay in the specimen processing and suboptimal storage of suspected samples in laboratories leads to degradation of SARS-CoV-2 viral RNA. Viral lysis buﬀers from RNA extraction kits have the po...

Descripción completa

Detalles Bibliográficos
Autores principales:	Perumal, Nagaraj, Jain, Rajeev Kumar, Shrivastava, Rakesh, Lalwani, Jaya, Chaurasia, Deepti
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Thieme Medical and Scientific Publishers Pvt. Ltd. 2020
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773443/ https://www.ncbi.nlm.nih.gov/pubmed/33390676 http://dx.doi.org/10.1055/s-0040-1722551

Ejemplares similares

Performance Evaluation of Different RT-PCR Kits for the Direct Detection of SARS-CoV-2 in Preheated Specimens
por: Jain, Rajeev Kumar, et al.
Publicado: (2023)

Efficacy Validation of SARS-CoV-2-Inactivation and Viral Genome Stability in Saliva by a Guanidine Hydrochloride and Surfactant-Based Virus Lysis/Transport Buffer
por: Komu, James Gitau, et al.
Publicado: (2023)

Lysis of tubercle bacilli in fresh and stored sputum specimens: implications for diagnosing tuberculosis in stored and paucibacillary specimens by PCR
por: Pathak, Divya, et al.
Publicado: (2007)

Virus Inactivation by Formaldehyde and Common Lysis Buffers
por: Seeburg, Ulrike, et al.
Publicado: (2023)

Improved sensitivity, safety, and rapidity of COVID-19 tests by replacing viral storage solution with lysis buffer
por: Erster, Oran, et al.
Publicado: (2021)

Emergence of Multidrug-Resistant Non-Fermenting Gram-Negative Bacilli in a Tertiary Care Teaching Hospital of Central India: Is Colistin Resistance Still a Distant Threat?
por: Soni, Mitisha, et al.
Publicado: (2023)

Evaluation of bacterial co-infections in COVID-19 patients admitted in ICU
por: Rathore, Neha, et al.
Publicado: (2021)

Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
por: Castellanos-Gonzalez, Alejandro, et al.
Publicado: (2021)

Performance Analysis of Different Real Time-Pcr Kits for the Diagnosis of Sars-Cov-2 Virus
por: GANVIR, R.U.C.H.I., et al.
Publicado: (2021)

Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer
por: Guerra, Victor, et al.
Publicado: (2020)

Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer
por: Fazeli, Behnaz, et al.
Publicado: (2019)

Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection
por: Perchetti, Garrett A., et al.
Publicado: (2020)

Paradoxical buffering of calcium by calsequestrin demonstrated for the calcium store of skeletal muscle
por: Royer, Leandro, et al.
Publicado: (2010)

Investigation of SARS-CoV-2 RNAemia in the convalescent plasma of COVID-19 patients
por: Perumal, N., et al.
Publicado: (2022)

Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
por: Chaurasia, Rajesh K., et al.
Publicado: (2021)

Divalent cations and molecular crowding buffers stabilize G-triplex at physiologically relevant temperatures
por: Jiang, Hong-Xin, et al.
Publicado: (2015)

Storage stability of liposomes stored at elevated subzero temperatures in DMSO/sucrose mixtures
por: Sydykov, Bulat, et al.
Publicado: (2018)

A Simple Method for DNA Extraction from Mature Date Palm Leaves: Impact of Sand Grinding and Composition of Lysis Buffer
por: Arif, Ibrahim A., et al.
Publicado: (2010)

Comparing Efficiency of Lysis Buffer Solutions and Sample Preparation Methods for Liquid Chromatography–Mass Spectrometry Analysis of Human Cells and Plasma
por: Neset, Lasse, et al.
Publicado: (2022)

Rate of shed of SARS COV-2 viral RNA from COVID-19 cadavers
por: Sharma, Meenakshi, et al.
Publicado: (2022)

Viral invasion fitness across a continuum from lysis to latency
por: Weitz, Joshua S, et al.
Publicado: (2019)

Predictive Evaluation of Microbiological Stability of Soft Drinks with Lycium barbarum L. Stored at Temperature Shifts
por: Plucińska, Aleksandra, et al.
Publicado: (2022)

Mechanisms responsible for F-actin stabilization after lysis of polymorphonuclear leukocytes
Publicado: (1992)

Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater
por: Rodríguez, Antonio, et al.
Publicado: (2019)

Classification of SARS-CoV-2 viral genome sequences using Neurochaos Learning
por: Harikrishnan, N. B., et al.
Publicado: (2022)

Viral Lysis of Photosynthesizing Microbes As a Mechanism for Calcium Carbonate Nucleation in Seawater
por: Lisle, John T., et al.
Publicado: (2016)

Viral lysis modifies seasonal phytoplankton dynamics and carbon flow in the Southern Ocean
por: Biggs, Tristan E. G., et al.
Publicado: (2021)

Ecological Importance of Viral Lysis as a Loss Factor of Phytoplankton in the Amundsen Sea
por: Eich, Charlotte, et al.
Publicado: (2022)

Utility of Ethylene-Diamine-Tetraacetic Acid Buffer Solution With Boric Acid for Immunostaining of Specimens Stored for an Extended Period
por: Hatta, Hideki, et al.
Publicado: (2021)

Effect of time and temperature on stability of progestagens, testosterone and cortisol in Asian elephant blood stored with and without anticoagulant
por: Khonmee, Jaruwan, et al.
Publicado: (2019)

Temperature-dependent surface stability of SARS-CoV-2
por: Kratzel, Annika, et al.
Publicado: (2020)

Quantum dot-induced viral capsid assembling in dissociation buffer
por: Gao, Ding, et al.
Publicado: (2013)

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins
por: Leibly, David J., et al.
Publicado: (2012)

Temperature induces changes in Drosophila energy stores
por: Klepsatel, Peter, et al.
Publicado: (2019)

Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers
por: Ngoka, Lambert CM
Publicado: (2008)

Flavour stability of sterilised chickpeas stored in pouches
por: Noordraven, Laura E.C., et al.
Publicado: (2021)

Evaluation of Saline, Phosphate-Buffered Saline, and Minimum Essential Medium as Potential Alternatives to Viral Transport Media for SARS-CoV-2 Testing
por: Rodino, Kyle G., et al.
Publicado: (2020)

Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads
por: Bae, Minju, et al.
Publicado: (2022)

Buffered Qualitative Stability explains the robustness and evolvability of transcriptional networks
por: Albergante, Luca, et al.
Publicado: (2014)

Coupled power generators require stability buffers in addition to inertia
por: Raman, Gurupraanesh, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6e539vg7i3qnknhksdtr6hdc6m