Cargando…
Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation
Cerebellar neurons can signal sensory and motor events, but their role in active sensorimotor processing remains unclear. We record and manipulate Purkinje cell activity during a task that requires mice to rapidly discriminate between multisensory and unisensory stimuli before motor initiation. Neur...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773552/ https://www.ncbi.nlm.nih.gov/pubmed/33357441 http://dx.doi.org/10.1016/j.celrep.2020.108537 |
_version_ | 1783630065802674176 |
---|---|
author | Tsutsumi, Shinichiro Chadney, Oscar Yiu, Tin-Long Bäumler, Edgar Faraggiana, Lavinia Beau, Maxime Häusser, Michael |
author_facet | Tsutsumi, Shinichiro Chadney, Oscar Yiu, Tin-Long Bäumler, Edgar Faraggiana, Lavinia Beau, Maxime Häusser, Michael |
author_sort | Tsutsumi, Shinichiro |
collection | PubMed |
description | Cerebellar neurons can signal sensory and motor events, but their role in active sensorimotor processing remains unclear. We record and manipulate Purkinje cell activity during a task that requires mice to rapidly discriminate between multisensory and unisensory stimuli before motor initiation. Neuropixels recordings show that both sensory stimuli and motor initiation are represented by short-latency simple spikes. Optogenetic manipulation of short-latency simple spikes abolishes or delays motor initiation in a rate-dependent manner, indicating a role in motor initiation and its timing. Two-photon calcium imaging reveals task-related coherence of complex spikes organized into conserved alternating parasagittal stripes. The coherence of sensory-evoked complex spikes increases with learning and correlates with enhanced temporal precision of motor initiation. These results suggest that both simple spikes and complex spikes govern sensory-driven motor initiation: simple spikes modulate its latency, and complex spikes refine its temporal precision, providing specific cellular substrates for cerebellar sensorimotor control. |
format | Online Article Text |
id | pubmed-7773552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-77735522021-01-05 Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation Tsutsumi, Shinichiro Chadney, Oscar Yiu, Tin-Long Bäumler, Edgar Faraggiana, Lavinia Beau, Maxime Häusser, Michael Cell Rep Article Cerebellar neurons can signal sensory and motor events, but their role in active sensorimotor processing remains unclear. We record and manipulate Purkinje cell activity during a task that requires mice to rapidly discriminate between multisensory and unisensory stimuli before motor initiation. Neuropixels recordings show that both sensory stimuli and motor initiation are represented by short-latency simple spikes. Optogenetic manipulation of short-latency simple spikes abolishes or delays motor initiation in a rate-dependent manner, indicating a role in motor initiation and its timing. Two-photon calcium imaging reveals task-related coherence of complex spikes organized into conserved alternating parasagittal stripes. The coherence of sensory-evoked complex spikes increases with learning and correlates with enhanced temporal precision of motor initiation. These results suggest that both simple spikes and complex spikes govern sensory-driven motor initiation: simple spikes modulate its latency, and complex spikes refine its temporal precision, providing specific cellular substrates for cerebellar sensorimotor control. Cell Press 2020-12-22 /pmc/articles/PMC7773552/ /pubmed/33357441 http://dx.doi.org/10.1016/j.celrep.2020.108537 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tsutsumi, Shinichiro Chadney, Oscar Yiu, Tin-Long Bäumler, Edgar Faraggiana, Lavinia Beau, Maxime Häusser, Michael Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation |
title | Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation |
title_full | Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation |
title_fullStr | Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation |
title_full_unstemmed | Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation |
title_short | Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation |
title_sort | purkinje cell activity determines the timing of sensory-evoked motor initiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773552/ https://www.ncbi.nlm.nih.gov/pubmed/33357441 http://dx.doi.org/10.1016/j.celrep.2020.108537 |
work_keys_str_mv | AT tsutsumishinichiro purkinjecellactivitydeterminesthetimingofsensoryevokedmotorinitiation AT chadneyoscar purkinjecellactivitydeterminesthetimingofsensoryevokedmotorinitiation AT yiutinlong purkinjecellactivitydeterminesthetimingofsensoryevokedmotorinitiation AT baumleredgar purkinjecellactivitydeterminesthetimingofsensoryevokedmotorinitiation AT faraggianalavinia purkinjecellactivitydeterminesthetimingofsensoryevokedmotorinitiation AT beaumaxime purkinjecellactivitydeterminesthetimingofsensoryevokedmotorinitiation AT haussermichael purkinjecellactivitydeterminesthetimingofsensoryevokedmotorinitiation |