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Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates

Currently, H9N2 avian influenza viruses (H9N2 AIVs) globally circulate in poultry and have acquired some adaptation to mammals. However, it is not clear what the molecular basis is for the variation in receptor-binding features of the H9N2 AIVs. The receptor-binding features of 92 H9N2 AIVs prevalen...

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Autores principales: Liu, Yang, Li, Shuo, Sun, Huapeng, Pan, Liangqi, Cui, Xinxin, Zhu, Xuhui, Feng, Yaling, Li, Mingliang, Yu, Yanan, Wu, Meihua, Lin, Jiate, Xu, Fengxiang, Yuan, Shaohua, Huang, Shujian, Sun, Hailiang, Liao, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773702/
https://www.ncbi.nlm.nih.gov/pubmed/33391219
http://dx.doi.org/10.3389/fmicb.2020.602124
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author Liu, Yang
Li, Shuo
Sun, Huapeng
Pan, Liangqi
Cui, Xinxin
Zhu, Xuhui
Feng, Yaling
Li, Mingliang
Yu, Yanan
Wu, Meihua
Lin, Jiate
Xu, Fengxiang
Yuan, Shaohua
Huang, Shujian
Sun, Hailiang
Liao, Ming
author_facet Liu, Yang
Li, Shuo
Sun, Huapeng
Pan, Liangqi
Cui, Xinxin
Zhu, Xuhui
Feng, Yaling
Li, Mingliang
Yu, Yanan
Wu, Meihua
Lin, Jiate
Xu, Fengxiang
Yuan, Shaohua
Huang, Shujian
Sun, Hailiang
Liao, Ming
author_sort Liu, Yang
collection PubMed
description Currently, H9N2 avian influenza viruses (H9N2 AIVs) globally circulate in poultry and have acquired some adaptation to mammals. However, it is not clear what the molecular basis is for the variation in receptor-binding features of the H9N2 AIVs. The receptor-binding features of 92 H9N2 AIVs prevalent in China during 1994–2017 were characterized through solid-phase ELISA assay and reverse genetics. H9N2 AIVs that circulated in this period mostly belonged to clade h9.4.2. Two increasing incidents occurred in the ability of H9N2 AIVs to bind to avian-like receptors in 2002–2005 and 2011–2014. Two increasing incidents occurred in the strength of H9N2 AIVs to bind to human-like receptors in 2002–2005 and 2011–2017. We found that Q227M, D145G/N, S119R, and R246K mutations can significantly increase H9N2 AIVs to bind to both avian- and human-like receptors. A160D/N, Q156R, T205A, Q226L, V245I, V216L, D208E, T212I, R172Q, and S175N mutations can significantly enhance the strength of H9N2 AIVs to bind to human-like receptors. Our study also identified mutations T205A, D208E, V216L, Q226L, and V245I as the key sites leading to enhanced receptor binding of H9N2 AIVs during 2002–2005 and mutations S119R, D145G, Q156R, A160D, T212I, Q227M, and R246K as the key sites leading to enhanced receptor binding of H9N2 AIVs during 2011–2017. These findings further illustrate the receptor-binding characteristics of avian influenza viruses, which can be a potential threat to public health.
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spelling pubmed-77737022021-01-01 Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates Liu, Yang Li, Shuo Sun, Huapeng Pan, Liangqi Cui, Xinxin Zhu, Xuhui Feng, Yaling Li, Mingliang Yu, Yanan Wu, Meihua Lin, Jiate Xu, Fengxiang Yuan, Shaohua Huang, Shujian Sun, Hailiang Liao, Ming Front Microbiol Microbiology Currently, H9N2 avian influenza viruses (H9N2 AIVs) globally circulate in poultry and have acquired some adaptation to mammals. However, it is not clear what the molecular basis is for the variation in receptor-binding features of the H9N2 AIVs. The receptor-binding features of 92 H9N2 AIVs prevalent in China during 1994–2017 were characterized through solid-phase ELISA assay and reverse genetics. H9N2 AIVs that circulated in this period mostly belonged to clade h9.4.2. Two increasing incidents occurred in the ability of H9N2 AIVs to bind to avian-like receptors in 2002–2005 and 2011–2014. Two increasing incidents occurred in the strength of H9N2 AIVs to bind to human-like receptors in 2002–2005 and 2011–2017. We found that Q227M, D145G/N, S119R, and R246K mutations can significantly increase H9N2 AIVs to bind to both avian- and human-like receptors. A160D/N, Q156R, T205A, Q226L, V245I, V216L, D208E, T212I, R172Q, and S175N mutations can significantly enhance the strength of H9N2 AIVs to bind to human-like receptors. Our study also identified mutations T205A, D208E, V216L, Q226L, and V245I as the key sites leading to enhanced receptor binding of H9N2 AIVs during 2002–2005 and mutations S119R, D145G, Q156R, A160D, T212I, Q227M, and R246K as the key sites leading to enhanced receptor binding of H9N2 AIVs during 2011–2017. These findings further illustrate the receptor-binding characteristics of avian influenza viruses, which can be a potential threat to public health. Frontiers Media S.A. 2020-12-17 /pmc/articles/PMC7773702/ /pubmed/33391219 http://dx.doi.org/10.3389/fmicb.2020.602124 Text en Copyright © 2020 Liu, Li, Sun, Pan, Cui, Zhu, Feng, Li, Yu, Wu, Lin, Xu, Yuan, Huang, Sun and Liao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Yang
Li, Shuo
Sun, Huapeng
Pan, Liangqi
Cui, Xinxin
Zhu, Xuhui
Feng, Yaling
Li, Mingliang
Yu, Yanan
Wu, Meihua
Lin, Jiate
Xu, Fengxiang
Yuan, Shaohua
Huang, Shujian
Sun, Hailiang
Liao, Ming
Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates
title Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates
title_full Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates
title_fullStr Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates
title_full_unstemmed Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates
title_short Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates
title_sort variation and molecular basis for enhancement of receptor binding of h9n2 avian influenza viruses in china isolates
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773702/
https://www.ncbi.nlm.nih.gov/pubmed/33391219
http://dx.doi.org/10.3389/fmicb.2020.602124
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