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Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential

INTRODUCTION: The p75 neurotrophin receptor (p75NTR) is known as an efficient marker for the prospective isolation of mesenchymal stem cells (MSCs) and neural crest-derived stem cells (NCSCs). To date, there is quite limited information concerning p75NTR-expressing cells in umbilical cord (UC), alth...

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Autores principales: Fujii-Tezuka, Rina, Ishige-Wada, Mika, Nagoshi, Narihito, Okano, Hideyuki, Mugishima, Hideo, Takahashi, Shori, Morioka, Ichiro, Matsumoto, Taro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773767/
https://www.ncbi.nlm.nih.gov/pubmed/33426237
http://dx.doi.org/10.1016/j.reth.2020.12.002
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author Fujii-Tezuka, Rina
Ishige-Wada, Mika
Nagoshi, Narihito
Okano, Hideyuki
Mugishima, Hideo
Takahashi, Shori
Morioka, Ichiro
Matsumoto, Taro
author_facet Fujii-Tezuka, Rina
Ishige-Wada, Mika
Nagoshi, Narihito
Okano, Hideyuki
Mugishima, Hideo
Takahashi, Shori
Morioka, Ichiro
Matsumoto, Taro
author_sort Fujii-Tezuka, Rina
collection PubMed
description INTRODUCTION: The p75 neurotrophin receptor (p75NTR) is known as an efficient marker for the prospective isolation of mesenchymal stem cells (MSCs) and neural crest-derived stem cells (NCSCs). To date, there is quite limited information concerning p75NTR-expressing cells in umbilical cord (UC), although UC is known as a rich source of MSCs. We show for the first time the localization, phenotype, and functional properties of p75NTR(+) cells in UC. METHODS: Human UC tissue sections were subjected to immunohistochemistry for MSC markers including p75NTR. Enzymatically isolated umbilical artery (UA) cells containing p75NTR(+) cells were assessed for immunophenotype, clonogenic capacity, and differentiation potential. To identify the presence of neural crest-derived cells in the UA, P0-Cre/Floxed-EGFP reporter mouse embryos were used, and immunohistochemical analysis of UC tissue was performed. RESULTS: Immunohistochemical analysis revealed that p75NTR(+) cells were specifically localized to the subendothelial area of the UA and umbilical vein. The p75NTR(+) cells co-expressed PDGFRβ, CD90, CD146, and NG2, phenotypic markers of MSCs and pericytes. Isolated UA cells possessed the potential to form neurospheres that further differentiated into neuronal and glial cell lineages. Genetic lineage tracing analysis showed that EGFP(+) neural crest-derived cells were detected in the subendothelial area of UA with p75NTR immunoreactivity. CONCLUSIONS: These results show that UA tissue harbors p75NTR(+) pericyte-like cells in the subendothelial area that have the capacity to form neurospheres and the potential for neurogenic differentiation. The lineage tracing data suggests the p75NTR(+) cells are putatively derived from the neural crest.
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spelling pubmed-77737672021-01-08 Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential Fujii-Tezuka, Rina Ishige-Wada, Mika Nagoshi, Narihito Okano, Hideyuki Mugishima, Hideo Takahashi, Shori Morioka, Ichiro Matsumoto, Taro Regen Ther Original Article INTRODUCTION: The p75 neurotrophin receptor (p75NTR) is known as an efficient marker for the prospective isolation of mesenchymal stem cells (MSCs) and neural crest-derived stem cells (NCSCs). To date, there is quite limited information concerning p75NTR-expressing cells in umbilical cord (UC), although UC is known as a rich source of MSCs. We show for the first time the localization, phenotype, and functional properties of p75NTR(+) cells in UC. METHODS: Human UC tissue sections were subjected to immunohistochemistry for MSC markers including p75NTR. Enzymatically isolated umbilical artery (UA) cells containing p75NTR(+) cells were assessed for immunophenotype, clonogenic capacity, and differentiation potential. To identify the presence of neural crest-derived cells in the UA, P0-Cre/Floxed-EGFP reporter mouse embryos were used, and immunohistochemical analysis of UC tissue was performed. RESULTS: Immunohistochemical analysis revealed that p75NTR(+) cells were specifically localized to the subendothelial area of the UA and umbilical vein. The p75NTR(+) cells co-expressed PDGFRβ, CD90, CD146, and NG2, phenotypic markers of MSCs and pericytes. Isolated UA cells possessed the potential to form neurospheres that further differentiated into neuronal and glial cell lineages. Genetic lineage tracing analysis showed that EGFP(+) neural crest-derived cells were detected in the subendothelial area of UA with p75NTR immunoreactivity. CONCLUSIONS: These results show that UA tissue harbors p75NTR(+) pericyte-like cells in the subendothelial area that have the capacity to form neurospheres and the potential for neurogenic differentiation. The lineage tracing data suggests the p75NTR(+) cells are putatively derived from the neural crest. Japanese Society for Regenerative Medicine 2020-12-24 /pmc/articles/PMC7773767/ /pubmed/33426237 http://dx.doi.org/10.1016/j.reth.2020.12.002 Text en © 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Fujii-Tezuka, Rina
Ishige-Wada, Mika
Nagoshi, Narihito
Okano, Hideyuki
Mugishima, Hideo
Takahashi, Shori
Morioka, Ichiro
Matsumoto, Taro
Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential
title Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential
title_full Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential
title_fullStr Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential
title_full_unstemmed Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential
title_short Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential
title_sort umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773767/
https://www.ncbi.nlm.nih.gov/pubmed/33426237
http://dx.doi.org/10.1016/j.reth.2020.12.002
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