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Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model
Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron s...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773786/ https://www.ncbi.nlm.nih.gov/pubmed/33392192 http://dx.doi.org/10.3389/fcell.2020.603392 |
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author | Chen, Shuai Wu, Xin Wang, Xia Shao, Yirui Tu, Qiang Yang, Huansheng Yin, Jie Yin, Yulong |
author_facet | Chen, Shuai Wu, Xin Wang, Xia Shao, Yirui Tu, Qiang Yang, Huansheng Yin, Jie Yin, Yulong |
author_sort | Chen, Shuai |
collection | PubMed |
description | Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron supplementation on growth performance, the distribution pattern of iron in the gut lumen and the host, intestinal microbiota, and intestine transcript profile of piglets. Sixty healthy weaning piglets were randomly assigned to six groups: fed on diets supplemented with ferrous sulfate monohydrate at the dose of 50 ppm (Fe50 group), 100 ppm (Fe100 group), 200 ppm (Fe200 group), 500 ppm (Fe500 group), and 800 ppm (Fe800), separately, for 3 weeks. The results indicated that increasing iron had no significant effects on growth performance, but increased diarrheal risk and iron deposition in intestinal digesta, tissues of intestine and liver, and serum. High iron also reduced serum iron-binding capacity, apolipoprotein, and immunoglobin A. The RNA-sequencing analysis revealed that iron changed colonic transcript profile, such as interferon gamma-signal transducer and activator of transcription two-based anti-infection gene network. Increasing iron also shifted colonic and cecal microbiota, such as reducing alpha diversity and the relative abundance of Clostridiales and Lactobacillus reuteri and increasing the relative abundance of Lactobacillus and Lactobacillus amylovorus. Collectively, this study demonstrated that high dietary iron increased diarrheal incidence, changed intestinal immune response-associated gene expression, and shifted gut microbiota. The results would enhance our knowledge of iron effects on the gut and microbiome in piglets and further contribute to understanding these aspects in humans. |
format | Online Article Text |
id | pubmed-7773786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77737862021-01-01 Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model Chen, Shuai Wu, Xin Wang, Xia Shao, Yirui Tu, Qiang Yang, Huansheng Yin, Jie Yin, Yulong Front Cell Dev Biol Cell and Developmental Biology Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron supplementation on growth performance, the distribution pattern of iron in the gut lumen and the host, intestinal microbiota, and intestine transcript profile of piglets. Sixty healthy weaning piglets were randomly assigned to six groups: fed on diets supplemented with ferrous sulfate monohydrate at the dose of 50 ppm (Fe50 group), 100 ppm (Fe100 group), 200 ppm (Fe200 group), 500 ppm (Fe500 group), and 800 ppm (Fe800), separately, for 3 weeks. The results indicated that increasing iron had no significant effects on growth performance, but increased diarrheal risk and iron deposition in intestinal digesta, tissues of intestine and liver, and serum. High iron also reduced serum iron-binding capacity, apolipoprotein, and immunoglobin A. The RNA-sequencing analysis revealed that iron changed colonic transcript profile, such as interferon gamma-signal transducer and activator of transcription two-based anti-infection gene network. Increasing iron also shifted colonic and cecal microbiota, such as reducing alpha diversity and the relative abundance of Clostridiales and Lactobacillus reuteri and increasing the relative abundance of Lactobacillus and Lactobacillus amylovorus. Collectively, this study demonstrated that high dietary iron increased diarrheal incidence, changed intestinal immune response-associated gene expression, and shifted gut microbiota. The results would enhance our knowledge of iron effects on the gut and microbiome in piglets and further contribute to understanding these aspects in humans. Frontiers Media S.A. 2020-12-17 /pmc/articles/PMC7773786/ /pubmed/33392192 http://dx.doi.org/10.3389/fcell.2020.603392 Text en Copyright © 2020 Chen, Wu, Wang, Shao, Tu, Yang, Yin and Yin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Chen, Shuai Wu, Xin Wang, Xia Shao, Yirui Tu, Qiang Yang, Huansheng Yin, Jie Yin, Yulong Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model |
title | Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model |
title_full | Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model |
title_fullStr | Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model |
title_full_unstemmed | Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model |
title_short | Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model |
title_sort | responses of intestinal microbiota and immunity to increasing dietary levels of iron using a piglet model |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773786/ https://www.ncbi.nlm.nih.gov/pubmed/33392192 http://dx.doi.org/10.3389/fcell.2020.603392 |
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