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Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron s...

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Autores principales: Chen, Shuai, Wu, Xin, Wang, Xia, Shao, Yirui, Tu, Qiang, Yang, Huansheng, Yin, Jie, Yin, Yulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773786/
https://www.ncbi.nlm.nih.gov/pubmed/33392192
http://dx.doi.org/10.3389/fcell.2020.603392
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author Chen, Shuai
Wu, Xin
Wang, Xia
Shao, Yirui
Tu, Qiang
Yang, Huansheng
Yin, Jie
Yin, Yulong
author_facet Chen, Shuai
Wu, Xin
Wang, Xia
Shao, Yirui
Tu, Qiang
Yang, Huansheng
Yin, Jie
Yin, Yulong
author_sort Chen, Shuai
collection PubMed
description Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron supplementation on growth performance, the distribution pattern of iron in the gut lumen and the host, intestinal microbiota, and intestine transcript profile of piglets. Sixty healthy weaning piglets were randomly assigned to six groups: fed on diets supplemented with ferrous sulfate monohydrate at the dose of 50 ppm (Fe50 group), 100 ppm (Fe100 group), 200 ppm (Fe200 group), 500 ppm (Fe500 group), and 800 ppm (Fe800), separately, for 3 weeks. The results indicated that increasing iron had no significant effects on growth performance, but increased diarrheal risk and iron deposition in intestinal digesta, tissues of intestine and liver, and serum. High iron also reduced serum iron-binding capacity, apolipoprotein, and immunoglobin A. The RNA-sequencing analysis revealed that iron changed colonic transcript profile, such as interferon gamma-signal transducer and activator of transcription two-based anti-infection gene network. Increasing iron also shifted colonic and cecal microbiota, such as reducing alpha diversity and the relative abundance of Clostridiales and Lactobacillus reuteri and increasing the relative abundance of Lactobacillus and Lactobacillus amylovorus. Collectively, this study demonstrated that high dietary iron increased diarrheal incidence, changed intestinal immune response-associated gene expression, and shifted gut microbiota. The results would enhance our knowledge of iron effects on the gut and microbiome in piglets and further contribute to understanding these aspects in humans.
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spelling pubmed-77737862021-01-01 Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model Chen, Shuai Wu, Xin Wang, Xia Shao, Yirui Tu, Qiang Yang, Huansheng Yin, Jie Yin, Yulong Front Cell Dev Biol Cell and Developmental Biology Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron supplementation on growth performance, the distribution pattern of iron in the gut lumen and the host, intestinal microbiota, and intestine transcript profile of piglets. Sixty healthy weaning piglets were randomly assigned to six groups: fed on diets supplemented with ferrous sulfate monohydrate at the dose of 50 ppm (Fe50 group), 100 ppm (Fe100 group), 200 ppm (Fe200 group), 500 ppm (Fe500 group), and 800 ppm (Fe800), separately, for 3 weeks. The results indicated that increasing iron had no significant effects on growth performance, but increased diarrheal risk and iron deposition in intestinal digesta, tissues of intestine and liver, and serum. High iron also reduced serum iron-binding capacity, apolipoprotein, and immunoglobin A. The RNA-sequencing analysis revealed that iron changed colonic transcript profile, such as interferon gamma-signal transducer and activator of transcription two-based anti-infection gene network. Increasing iron also shifted colonic and cecal microbiota, such as reducing alpha diversity and the relative abundance of Clostridiales and Lactobacillus reuteri and increasing the relative abundance of Lactobacillus and Lactobacillus amylovorus. Collectively, this study demonstrated that high dietary iron increased diarrheal incidence, changed intestinal immune response-associated gene expression, and shifted gut microbiota. The results would enhance our knowledge of iron effects on the gut and microbiome in piglets and further contribute to understanding these aspects in humans. Frontiers Media S.A. 2020-12-17 /pmc/articles/PMC7773786/ /pubmed/33392192 http://dx.doi.org/10.3389/fcell.2020.603392 Text en Copyright © 2020 Chen, Wu, Wang, Shao, Tu, Yang, Yin and Yin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Chen, Shuai
Wu, Xin
Wang, Xia
Shao, Yirui
Tu, Qiang
Yang, Huansheng
Yin, Jie
Yin, Yulong
Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model
title Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model
title_full Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model
title_fullStr Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model
title_full_unstemmed Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model
title_short Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model
title_sort responses of intestinal microbiota and immunity to increasing dietary levels of iron using a piglet model
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773786/
https://www.ncbi.nlm.nih.gov/pubmed/33392192
http://dx.doi.org/10.3389/fcell.2020.603392
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