Cargando…
Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping
Stem rust of wheat caused by Puccinia graminis Pers. f.sp. trtici Eriks and E. Henn., is the most damaging fungal disease of both common (Triticum aestivum L.) and durum (Triticum turgidum L., ssp. Durum) wheat. Continuously emerging races virulent to many of the commercially deployed qualitative re...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773921/ https://www.ncbi.nlm.nih.gov/pubmed/33391309 http://dx.doi.org/10.3389/fpls.2020.598509 |
_version_ | 1783630153340944384 |
---|---|
author | Megerssa, Shitaye H. Ammar, Karim Acevedo, Maricelis Brown-Guedira, Gina Ward, Brian Degete, Ashenafi G. Randhawa, Mandeep S. Sorrells, Mark E. |
author_facet | Megerssa, Shitaye H. Ammar, Karim Acevedo, Maricelis Brown-Guedira, Gina Ward, Brian Degete, Ashenafi G. Randhawa, Mandeep S. Sorrells, Mark E. |
author_sort | Megerssa, Shitaye H. |
collection | PubMed |
description | Stem rust of wheat caused by Puccinia graminis Pers. f.sp. trtici Eriks and E. Henn., is the most damaging fungal disease of both common (Triticum aestivum L.) and durum (Triticum turgidum L., ssp. Durum) wheat. Continuously emerging races virulent to many of the commercially deployed qualitative resistance genes have caused remarkable loss worldwide and threaten global wheat production. The objectives of this study were to evaluate the response of a panel of 283 durum wheat lines assembled by the International Maize and Wheat Improvement Center (CIMMYT) to multiple races of stem rust in East Africa at the adult plant stage and map loci associated with field resistance. The lines were evaluated in Debre Zeit, Ethiopia and Njoro, Kenya from 2018 to 2019 in five environments (year × season). The panel was genotyped using genotyping-by-sequencing. After filtering, 26,439 Single Nucleotide Polymorphism (SNP) markers and 280 lines and three checks were retained for analysis. Population structure was assessed using principal component analysis. Genome-wide association analysis (GWAS) was conducted using Genomic Association and Prediction Integrated Tool (GAPIT). The broad-sense heritability of the phenotype data revealed that 64–83% of the variation in stem rust response explained by the genotypes and lines with multiple race resistance were identified. GWAS analysis detected a total of 160 significant marker trait associations representing 42 quantitative trait loci. Of those, 21 were potentially novel and 21 were mapped to the same regions as previously reported loci. Known stem rust resistance genes/alleles were postulated including Sr8a, Sr8155B1, SrWeb/Sr9h, Sr11, Sr12, Sr13/Sr13 alleles, Sr17, Sr28/Sr16, Sr22, and Sr49. Lines resistant to multiple races in East Africa can be utilized as parents in durum wheat breeding programs. Further studies are needed to determine if there are new alleles at the Sr13 locus and potential markers for the known Sr13 alleles. |
format | Online Article Text |
id | pubmed-7773921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77739212021-01-01 Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping Megerssa, Shitaye H. Ammar, Karim Acevedo, Maricelis Brown-Guedira, Gina Ward, Brian Degete, Ashenafi G. Randhawa, Mandeep S. Sorrells, Mark E. Front Plant Sci Plant Science Stem rust of wheat caused by Puccinia graminis Pers. f.sp. trtici Eriks and E. Henn., is the most damaging fungal disease of both common (Triticum aestivum L.) and durum (Triticum turgidum L., ssp. Durum) wheat. Continuously emerging races virulent to many of the commercially deployed qualitative resistance genes have caused remarkable loss worldwide and threaten global wheat production. The objectives of this study were to evaluate the response of a panel of 283 durum wheat lines assembled by the International Maize and Wheat Improvement Center (CIMMYT) to multiple races of stem rust in East Africa at the adult plant stage and map loci associated with field resistance. The lines were evaluated in Debre Zeit, Ethiopia and Njoro, Kenya from 2018 to 2019 in five environments (year × season). The panel was genotyped using genotyping-by-sequencing. After filtering, 26,439 Single Nucleotide Polymorphism (SNP) markers and 280 lines and three checks were retained for analysis. Population structure was assessed using principal component analysis. Genome-wide association analysis (GWAS) was conducted using Genomic Association and Prediction Integrated Tool (GAPIT). The broad-sense heritability of the phenotype data revealed that 64–83% of the variation in stem rust response explained by the genotypes and lines with multiple race resistance were identified. GWAS analysis detected a total of 160 significant marker trait associations representing 42 quantitative trait loci. Of those, 21 were potentially novel and 21 were mapped to the same regions as previously reported loci. Known stem rust resistance genes/alleles were postulated including Sr8a, Sr8155B1, SrWeb/Sr9h, Sr11, Sr12, Sr13/Sr13 alleles, Sr17, Sr28/Sr16, Sr22, and Sr49. Lines resistant to multiple races in East Africa can be utilized as parents in durum wheat breeding programs. Further studies are needed to determine if there are new alleles at the Sr13 locus and potential markers for the known Sr13 alleles. Frontiers Media S.A. 2020-12-17 /pmc/articles/PMC7773921/ /pubmed/33391309 http://dx.doi.org/10.3389/fpls.2020.598509 Text en Copyright © 2020 Megerssa, Ammar, Acevedo, Brown-Guedira, Ward, Degete, Randhawa and Sorrells. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Megerssa, Shitaye H. Ammar, Karim Acevedo, Maricelis Brown-Guedira, Gina Ward, Brian Degete, Ashenafi G. Randhawa, Mandeep S. Sorrells, Mark E. Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping |
title | Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping |
title_full | Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping |
title_fullStr | Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping |
title_full_unstemmed | Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping |
title_short | Multiple-Race Stem Rust Resistance Loci Identified in Durum Wheat Using Genome-Wide Association Mapping |
title_sort | multiple-race stem rust resistance loci identified in durum wheat using genome-wide association mapping |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773921/ https://www.ncbi.nlm.nih.gov/pubmed/33391309 http://dx.doi.org/10.3389/fpls.2020.598509 |
work_keys_str_mv | AT megerssashitayeh multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping AT ammarkarim multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping AT acevedomaricelis multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping AT brownguediragina multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping AT wardbrian multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping AT degeteashenafig multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping AT randhawamandeeps multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping AT sorrellsmarke multipleracestemrustresistancelociidentifiedindurumwheatusinggenomewideassociationmapping |