Cargando…

Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats

Ethylene glycol monomethyl ether (EGME) has been used in many products usually handled by humans including inks, paints, polishes, brake fluids and so on. This present study therefore, investigated its effect on lung, in a time-course study in male Wistar rats. Animals were orally administered 50 mg...

Descripción completa

Detalles Bibliográficos
Autores principales: Somade, Oluwatobi T., Ajayi, Babajide O., Adeyi, Olubisi E., Adeshina, Anuoluwapo A., Adekoya, Mary O., Abdulhameed, Ridwan O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773962/
https://www.ncbi.nlm.nih.gov/pubmed/33409483
http://dx.doi.org/10.1016/j.metop.2020.100075
_version_ 1783630163182878720
author Somade, Oluwatobi T.
Ajayi, Babajide O.
Adeyi, Olubisi E.
Adeshina, Anuoluwapo A.
Adekoya, Mary O.
Abdulhameed, Ridwan O.
author_facet Somade, Oluwatobi T.
Ajayi, Babajide O.
Adeyi, Olubisi E.
Adeshina, Anuoluwapo A.
Adekoya, Mary O.
Abdulhameed, Ridwan O.
author_sort Somade, Oluwatobi T.
collection PubMed
description Ethylene glycol monomethyl ether (EGME) has been used in many products usually handled by humans including inks, paints, polishes, brake fluids and so on. This present study therefore, investigated its effect on lung, in a time-course study in male Wistar rats. Animals were orally administered 50 mg/kg body weight of EGME for a period of 7, 14, and 21 days. Following 7 days of oral exposure to EGME, activities of GPx and SOD were significantly increased, as well as levels of K-Ras, c-Myc, p53, caspase-3, TNF-α and, IL-6, while NO level and GST activity were significantly reduced compared with control. At the end of 14 days exposure, GSH level was significantly decreased, while levels of K-Ras, c-Myc, p53, caspase-3, TNF-α, IL-6, NO and the activities of SOD and GPx were significantly elevated with respect to control. After 21 days of EGME administration, levels of Bcl-2, IL-10, GSH and NO as well as GST activity were significantly decreased, while levels of K-Ras, c-Myc, p53, Bax, caspase-3, IL-6, IL-1β, TNF-α, as well as GPx, CAT, and SOD activities were significantly elevated compared with control. Lung histopathology revealed chronic disseminated alveolar inflammation, bronchiolitis, severe alveolar and bronchi hyperplasia, severe disseminated inflammation, thrombosis, and thickened vessels as a result of EGME exposures. Exposures to EGME could trigger lung damage via the disorganization of the antioxidant system, eliciting the up-regulation of inflammatory, apoptotic, and oncogenic markers in rats.
format Online
Article
Text
id pubmed-7773962
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-77739622021-01-05 Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats Somade, Oluwatobi T. Ajayi, Babajide O. Adeyi, Olubisi E. Adeshina, Anuoluwapo A. Adekoya, Mary O. Abdulhameed, Ridwan O. Metabol Open Original Research Paper Ethylene glycol monomethyl ether (EGME) has been used in many products usually handled by humans including inks, paints, polishes, brake fluids and so on. This present study therefore, investigated its effect on lung, in a time-course study in male Wistar rats. Animals were orally administered 50 mg/kg body weight of EGME for a period of 7, 14, and 21 days. Following 7 days of oral exposure to EGME, activities of GPx and SOD were significantly increased, as well as levels of K-Ras, c-Myc, p53, caspase-3, TNF-α and, IL-6, while NO level and GST activity were significantly reduced compared with control. At the end of 14 days exposure, GSH level was significantly decreased, while levels of K-Ras, c-Myc, p53, caspase-3, TNF-α, IL-6, NO and the activities of SOD and GPx were significantly elevated with respect to control. After 21 days of EGME administration, levels of Bcl-2, IL-10, GSH and NO as well as GST activity were significantly decreased, while levels of K-Ras, c-Myc, p53, Bax, caspase-3, IL-6, IL-1β, TNF-α, as well as GPx, CAT, and SOD activities were significantly elevated compared with control. Lung histopathology revealed chronic disseminated alveolar inflammation, bronchiolitis, severe alveolar and bronchi hyperplasia, severe disseminated inflammation, thrombosis, and thickened vessels as a result of EGME exposures. Exposures to EGME could trigger lung damage via the disorganization of the antioxidant system, eliciting the up-regulation of inflammatory, apoptotic, and oncogenic markers in rats. Elsevier 2020-12-15 /pmc/articles/PMC7773962/ /pubmed/33409483 http://dx.doi.org/10.1016/j.metop.2020.100075 Text en © 2020 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Paper
Somade, Oluwatobi T.
Ajayi, Babajide O.
Adeyi, Olubisi E.
Adeshina, Anuoluwapo A.
Adekoya, Mary O.
Abdulhameed, Ridwan O.
Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats
title Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats
title_full Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats
title_fullStr Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats
title_full_unstemmed Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats
title_short Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats
title_sort oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773962/
https://www.ncbi.nlm.nih.gov/pubmed/33409483
http://dx.doi.org/10.1016/j.metop.2020.100075
work_keys_str_mv AT somadeoluwatobit oxidativestressmediatedinductionofpulmonaryoncogenesinflammatoryandapoptoticmarkersfollowingtimecourseexposuretoethyleneglycolmonomethyletherinrats
AT ajayibabajideo oxidativestressmediatedinductionofpulmonaryoncogenesinflammatoryandapoptoticmarkersfollowingtimecourseexposuretoethyleneglycolmonomethyletherinrats
AT adeyiolubisie oxidativestressmediatedinductionofpulmonaryoncogenesinflammatoryandapoptoticmarkersfollowingtimecourseexposuretoethyleneglycolmonomethyletherinrats
AT adeshinaanuoluwapoa oxidativestressmediatedinductionofpulmonaryoncogenesinflammatoryandapoptoticmarkersfollowingtimecourseexposuretoethyleneglycolmonomethyletherinrats
AT adekoyamaryo oxidativestressmediatedinductionofpulmonaryoncogenesinflammatoryandapoptoticmarkersfollowingtimecourseexposuretoethyleneglycolmonomethyletherinrats
AT abdulhameedridwano oxidativestressmediatedinductionofpulmonaryoncogenesinflammatoryandapoptoticmarkersfollowingtimecourseexposuretoethyleneglycolmonomethyletherinrats