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Adsorption of Amyloid Beta Peptide by Metal–Organic Frameworks
[Image: see text] Metal–organic frameworks (MOFs) are capable of adsorbing a wide range of molecules. In addition to the more commonly investigated small molecules, researchers have demonstrated that MOFs adsorb much larger molecules, such as proteins and peptides. We have investigated whether MOFs...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774084/ https://www.ncbi.nlm.nih.gov/pubmed/33403258 http://dx.doi.org/10.1021/acsomega.0c04019 |
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author | Mensinger, Zachary L. Cook, Brenna L. Wilson, Elsie L. |
author_facet | Mensinger, Zachary L. Cook, Brenna L. Wilson, Elsie L. |
author_sort | Mensinger, Zachary L. |
collection | PubMed |
description | [Image: see text] Metal–organic frameworks (MOFs) are capable of adsorbing a wide range of molecules. In addition to the more commonly investigated small molecules, researchers have demonstrated that MOFs adsorb much larger molecules, such as proteins and peptides. We have investigated whether MOFs are capable of adsorbing amyloid beta peptide. Amyloid beta plays a pivotal role in the progression of Alzheimer’s disease because individual copies of the peptides can aggregate, forming neurotoxic oligomers and the amyloid plaques found in brains of Alzheimer’s patients. After synthesizing a number of commonly studied MOFs, their adsorption capabilities were tested. We found that the MOFs tested readily adsorbed small amounts of amyloid beta (as determined by gel electrophoresis). It was determined that in most cases, adsorption occurs rapidly, with complete adsorption within minutes of incubation. Overall adsorption capacity was found to vary between different MOFs as well. Once adsorbed, amyloid beta peptide can subsequently be eluted from some MOFs by treatment with acetonitrile/water solutions, though retention strength varied between different MOFs. In some cases, MOFs that showed complete adsorption also saw high levels of peptide elution, but others showed little to no elution of the peptide. Together these data can help us begin to understand the interactions between amyloid beta and MOFs. |
format | Online Article Text |
id | pubmed-7774084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-77740842021-01-04 Adsorption of Amyloid Beta Peptide by Metal–Organic Frameworks Mensinger, Zachary L. Cook, Brenna L. Wilson, Elsie L. ACS Omega [Image: see text] Metal–organic frameworks (MOFs) are capable of adsorbing a wide range of molecules. In addition to the more commonly investigated small molecules, researchers have demonstrated that MOFs adsorb much larger molecules, such as proteins and peptides. We have investigated whether MOFs are capable of adsorbing amyloid beta peptide. Amyloid beta plays a pivotal role in the progression of Alzheimer’s disease because individual copies of the peptides can aggregate, forming neurotoxic oligomers and the amyloid plaques found in brains of Alzheimer’s patients. After synthesizing a number of commonly studied MOFs, their adsorption capabilities were tested. We found that the MOFs tested readily adsorbed small amounts of amyloid beta (as determined by gel electrophoresis). It was determined that in most cases, adsorption occurs rapidly, with complete adsorption within minutes of incubation. Overall adsorption capacity was found to vary between different MOFs as well. Once adsorbed, amyloid beta peptide can subsequently be eluted from some MOFs by treatment with acetonitrile/water solutions, though retention strength varied between different MOFs. In some cases, MOFs that showed complete adsorption also saw high levels of peptide elution, but others showed little to no elution of the peptide. Together these data can help us begin to understand the interactions between amyloid beta and MOFs. American Chemical Society 2020-12-14 /pmc/articles/PMC7774084/ /pubmed/33403258 http://dx.doi.org/10.1021/acsomega.0c04019 Text en © 2020 The Authors. Published by American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Mensinger, Zachary L. Cook, Brenna L. Wilson, Elsie L. Adsorption of Amyloid Beta Peptide by Metal–Organic Frameworks |
title | Adsorption of Amyloid Beta Peptide by Metal–Organic
Frameworks |
title_full | Adsorption of Amyloid Beta Peptide by Metal–Organic
Frameworks |
title_fullStr | Adsorption of Amyloid Beta Peptide by Metal–Organic
Frameworks |
title_full_unstemmed | Adsorption of Amyloid Beta Peptide by Metal–Organic
Frameworks |
title_short | Adsorption of Amyloid Beta Peptide by Metal–Organic
Frameworks |
title_sort | adsorption of amyloid beta peptide by metal–organic
frameworks |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774084/ https://www.ncbi.nlm.nih.gov/pubmed/33403258 http://dx.doi.org/10.1021/acsomega.0c04019 |
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