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UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats

[Image: see text] Nuciferine is an aporphine alkaloid monomer that is extracted from the leaves of the lotus species Nymphaea caerulea and Nelumbo nucifera Gaertn. Nuciferine was reported to treat cerebrovascular diseases. However, the potential mechanism of the neuroprotective effects of nuciferine...

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Autores principales: Wu, Lanlan, Chen, Chang, Li, Yongbiao, Guo, Cong, Fan, Yuqing, Yu, Dingrong, Zhang, Tinglan, Wen, Binyu, Yan, Zhiyong, Liu, An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774285/
https://www.ncbi.nlm.nih.gov/pubmed/33403305
http://dx.doi.org/10.1021/acsomega.0c05388
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author Wu, Lanlan
Chen, Chang
Li, Yongbiao
Guo, Cong
Fan, Yuqing
Yu, Dingrong
Zhang, Tinglan
Wen, Binyu
Yan, Zhiyong
Liu, An
author_facet Wu, Lanlan
Chen, Chang
Li, Yongbiao
Guo, Cong
Fan, Yuqing
Yu, Dingrong
Zhang, Tinglan
Wen, Binyu
Yan, Zhiyong
Liu, An
author_sort Wu, Lanlan
collection PubMed
description [Image: see text] Nuciferine is an aporphine alkaloid monomer that is extracted from the leaves of the lotus species Nymphaea caerulea and Nelumbo nucifera Gaertn. Nuciferine was reported to treat cerebrovascular diseases. However, the potential mechanism of the neuroprotective effects of nuciferine at the metabolomics level is still not unclear. The present research used neurological score, infarct volume, cerebral water content, and ultraperformance liquid chromatography to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based serum metabolomics to elucidate the anti-ischemic stroke effect and mechanisms of nuciferine. The results showed that nuciferine significantly improved neurological deficit scores and ameliorated cerebral edema and infarction. Multivariate data analysis methods were used to examine the differences in serum endogenous metabolism between groups, and the biomarkers of nuciferine on ischemic stroke were identified. Approximately 19 metabolites and 7 metabolic pathways associated with nuciferine on treatment of stroke were found, which indicated that nuciferine exerted a positive therapeutic action on cerebral ischemic by regulating metabolism. These results provided some data support for the study of anti-stroke effect of nuciferine from the perspective of metabolomics.
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spelling pubmed-77742852021-01-04 UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats Wu, Lanlan Chen, Chang Li, Yongbiao Guo, Cong Fan, Yuqing Yu, Dingrong Zhang, Tinglan Wen, Binyu Yan, Zhiyong Liu, An ACS Omega [Image: see text] Nuciferine is an aporphine alkaloid monomer that is extracted from the leaves of the lotus species Nymphaea caerulea and Nelumbo nucifera Gaertn. Nuciferine was reported to treat cerebrovascular diseases. However, the potential mechanism of the neuroprotective effects of nuciferine at the metabolomics level is still not unclear. The present research used neurological score, infarct volume, cerebral water content, and ultraperformance liquid chromatography to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based serum metabolomics to elucidate the anti-ischemic stroke effect and mechanisms of nuciferine. The results showed that nuciferine significantly improved neurological deficit scores and ameliorated cerebral edema and infarction. Multivariate data analysis methods were used to examine the differences in serum endogenous metabolism between groups, and the biomarkers of nuciferine on ischemic stroke were identified. Approximately 19 metabolites and 7 metabolic pathways associated with nuciferine on treatment of stroke were found, which indicated that nuciferine exerted a positive therapeutic action on cerebral ischemic by regulating metabolism. These results provided some data support for the study of anti-stroke effect of nuciferine from the perspective of metabolomics. American Chemical Society 2020-12-15 /pmc/articles/PMC7774285/ /pubmed/33403305 http://dx.doi.org/10.1021/acsomega.0c05388 Text en © 2020 The Authors. Published by American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Wu, Lanlan
Chen, Chang
Li, Yongbiao
Guo, Cong
Fan, Yuqing
Yu, Dingrong
Zhang, Tinglan
Wen, Binyu
Yan, Zhiyong
Liu, An
UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats
title UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats
title_full UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats
title_fullStr UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats
title_full_unstemmed UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats
title_short UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals the Anti-Ischemic Stroke Mechanism of Nuciferine in MCAO Rats
title_sort uplc-q-tof/ms-based serum metabolomics reveals the anti-ischemic stroke mechanism of nuciferine in mcao rats
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774285/
https://www.ncbi.nlm.nih.gov/pubmed/33403305
http://dx.doi.org/10.1021/acsomega.0c05388
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