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Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation

Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact...

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Autores principales: Chen, Bo, Ye, Diya, Luo, Lingling, Liu, Weirong, Peng, Kerong, Shu, Xiaoli, Gu, Weizhong, Wang, Xiaojun, Xiang, Charlie, Jiang, Mizu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774322/
https://www.ncbi.nlm.nih.gov/pubmed/33390964
http://dx.doi.org/10.3389/fphar.2020.588560
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author Chen, Bo
Ye, Diya
Luo, Lingling
Liu, Weirong
Peng, Kerong
Shu, Xiaoli
Gu, Weizhong
Wang, Xiaojun
Xiang, Charlie
Jiang, Mizu
author_facet Chen, Bo
Ye, Diya
Luo, Lingling
Liu, Weirong
Peng, Kerong
Shu, Xiaoli
Gu, Weizhong
Wang, Xiaojun
Xiang, Charlie
Jiang, Mizu
author_sort Chen, Bo
collection PubMed
description Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact with epithelial cells (ECs). Here, we found that there are adhesive bacteria attached to the Children's terminal ileum. And we compared the immune factors of non-adhesive bacteria in the children ileum with adhesive bacteria as well. Stimulated Th17 cell associated with adherent bacteria in the ileum ECs. SIgA responses are similar to those roles in mouse experiments. Immunohistochemical analysis confirmed that the expression of SAA1, IL-2, IL-17A, foxp3, RORγt, TGFβ, and protein increased in Th17 cells. Finally, we used 16S rRNA genes 454 pyrosequencing to analyze the differences in bacterial communities between adhesive and non-adhesive bacteria in the ileum. Ileum with adherent bacteria demonstrated increased mucosa-related bacteria, such as Clostridium, Ruminococcus, Veillonella, Butyricimonas, and Prevotella. We believe that adhesive bacteria in children’s terminal ileum associated with an increased Th17 cell activation and luminal secretory IgA. Adhesive bacteria very closely adhere to terminal ileum of children. They may play important role in human gut immunity and Crohn’s disease.
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spelling pubmed-77743222021-01-01 Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation Chen, Bo Ye, Diya Luo, Lingling Liu, Weirong Peng, Kerong Shu, Xiaoli Gu, Weizhong Wang, Xiaojun Xiang, Charlie Jiang, Mizu Front Pharmacol Original Research Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact with epithelial cells (ECs). Here, we found that there are adhesive bacteria attached to the Children's terminal ileum. And we compared the immune factors of non-adhesive bacteria in the children ileum with adhesive bacteria as well. Stimulated Th17 cell associated with adherent bacteria in the ileum ECs. SIgA responses are similar to those roles in mouse experiments. Immunohistochemical analysis confirmed that the expression of SAA1, IL-2, IL-17A, foxp3, RORγt, TGFβ, and protein increased in Th17 cells. Finally, we used 16S rRNA genes 454 pyrosequencing to analyze the differences in bacterial communities between adhesive and non-adhesive bacteria in the ileum. Ileum with adherent bacteria demonstrated increased mucosa-related bacteria, such as Clostridium, Ruminococcus, Veillonella, Butyricimonas, and Prevotella. We believe that adhesive bacteria in children’s terminal ileum associated with an increased Th17 cell activation and luminal secretory IgA. Adhesive bacteria very closely adhere to terminal ileum of children. They may play important role in human gut immunity and Crohn’s disease. Frontiers Media S.A. 2020-11-30 /pmc/articles/PMC7774322/ /pubmed/33390964 http://dx.doi.org/10.3389/fphar.2020.588560 Text en Copyright © 2020 Chen, Ye, Luo, Liu, Peng, Shu, Gu, Wang, Xiang and Jiang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Original Research
Chen, Bo
Ye, Diya
Luo, Lingling
Liu, Weirong
Peng, Kerong
Shu, Xiaoli
Gu, Weizhong
Wang, Xiaojun
Xiang, Charlie
Jiang, Mizu
Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation
title Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation
title_full Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation
title_fullStr Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation
title_full_unstemmed Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation
title_short Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation
title_sort adhesive bacteria in the terminal ileum of children correlates with increasing th17 cell activation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774322/
https://www.ncbi.nlm.nih.gov/pubmed/33390964
http://dx.doi.org/10.3389/fphar.2020.588560
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