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Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation
Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774322/ https://www.ncbi.nlm.nih.gov/pubmed/33390964 http://dx.doi.org/10.3389/fphar.2020.588560 |
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author | Chen, Bo Ye, Diya Luo, Lingling Liu, Weirong Peng, Kerong Shu, Xiaoli Gu, Weizhong Wang, Xiaojun Xiang, Charlie Jiang, Mizu |
author_facet | Chen, Bo Ye, Diya Luo, Lingling Liu, Weirong Peng, Kerong Shu, Xiaoli Gu, Weizhong Wang, Xiaojun Xiang, Charlie Jiang, Mizu |
author_sort | Chen, Bo |
collection | PubMed |
description | Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact with epithelial cells (ECs). Here, we found that there are adhesive bacteria attached to the Children's terminal ileum. And we compared the immune factors of non-adhesive bacteria in the children ileum with adhesive bacteria as well. Stimulated Th17 cell associated with adherent bacteria in the ileum ECs. SIgA responses are similar to those roles in mouse experiments. Immunohistochemical analysis confirmed that the expression of SAA1, IL-2, IL-17A, foxp3, RORγt, TGFβ, and protein increased in Th17 cells. Finally, we used 16S rRNA genes 454 pyrosequencing to analyze the differences in bacterial communities between adhesive and non-adhesive bacteria in the ileum. Ileum with adherent bacteria demonstrated increased mucosa-related bacteria, such as Clostridium, Ruminococcus, Veillonella, Butyricimonas, and Prevotella. We believe that adhesive bacteria in children’s terminal ileum associated with an increased Th17 cell activation and luminal secretory IgA. Adhesive bacteria very closely adhere to terminal ileum of children. They may play important role in human gut immunity and Crohn’s disease. |
format | Online Article Text |
id | pubmed-7774322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77743222021-01-01 Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation Chen, Bo Ye, Diya Luo, Lingling Liu, Weirong Peng, Kerong Shu, Xiaoli Gu, Weizhong Wang, Xiaojun Xiang, Charlie Jiang, Mizu Front Pharmacol Original Research Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact with epithelial cells (ECs). Here, we found that there are adhesive bacteria attached to the Children's terminal ileum. And we compared the immune factors of non-adhesive bacteria in the children ileum with adhesive bacteria as well. Stimulated Th17 cell associated with adherent bacteria in the ileum ECs. SIgA responses are similar to those roles in mouse experiments. Immunohistochemical analysis confirmed that the expression of SAA1, IL-2, IL-17A, foxp3, RORγt, TGFβ, and protein increased in Th17 cells. Finally, we used 16S rRNA genes 454 pyrosequencing to analyze the differences in bacterial communities between adhesive and non-adhesive bacteria in the ileum. Ileum with adherent bacteria demonstrated increased mucosa-related bacteria, such as Clostridium, Ruminococcus, Veillonella, Butyricimonas, and Prevotella. We believe that adhesive bacteria in children’s terminal ileum associated with an increased Th17 cell activation and luminal secretory IgA. Adhesive bacteria very closely adhere to terminal ileum of children. They may play important role in human gut immunity and Crohn’s disease. Frontiers Media S.A. 2020-11-30 /pmc/articles/PMC7774322/ /pubmed/33390964 http://dx.doi.org/10.3389/fphar.2020.588560 Text en Copyright © 2020 Chen, Ye, Luo, Liu, Peng, Shu, Gu, Wang, Xiang and Jiang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Original Research Chen, Bo Ye, Diya Luo, Lingling Liu, Weirong Peng, Kerong Shu, Xiaoli Gu, Weizhong Wang, Xiaojun Xiang, Charlie Jiang, Mizu Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation |
title | Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation |
title_full | Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation |
title_fullStr | Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation |
title_full_unstemmed | Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation |
title_short | Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation |
title_sort | adhesive bacteria in the terminal ileum of children correlates with increasing th17 cell activation |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774322/ https://www.ncbi.nlm.nih.gov/pubmed/33390964 http://dx.doi.org/10.3389/fphar.2020.588560 |
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