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Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis
BACKGROUND: Conventional protocols utilize core needle biopsy (CNB) or fine needle aspiration (FNA) to produce cell suspension for flow cytometry (FCM) is a diagnostic challenge for lymphoid malignancies. We aim to develop an alternative CNB rinsing technique (RT) to produce cell suspension for FCM...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774716/ https://www.ncbi.nlm.nih.gov/pubmed/33070470 http://dx.doi.org/10.1002/cam4.3540 |
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author | Chi, Pei‐Dong Liu, Yi‐Jun Huang, Yu‐Hua Mao, Ming‐Jie Wang, Yu Li, Zhi‐Ming Li, Jian |
author_facet | Chi, Pei‐Dong Liu, Yi‐Jun Huang, Yu‐Hua Mao, Ming‐Jie Wang, Yu Li, Zhi‐Ming Li, Jian |
author_sort | Chi, Pei‐Dong |
collection | PubMed |
description | BACKGROUND: Conventional protocols utilize core needle biopsy (CNB) or fine needle aspiration (FNA) to produce cell suspension for flow cytometry (FCM) is a diagnostic challenge for lymphoid malignancies. We aim to develop an alternative CNB rinsing technique (RT) to produce cell suspension for FCM during this mini‐invasive procedure of CNB for lymphoma diagnosis. METHODS: FNA and CNB specimens from the same lesion of 93 patients with suspected lymphoma were collected under the guidance of B‐ultrasound simultaneously. The fresh CNB samples were prepared to cell suspension by RT for FCM immunophenotyping analysis (Group CNB‐RT). Then, the CNB tissues after performing the RT process and the fresh FNA tissues were processed by conventional tissue cell suspension (TCS) technique to obtain the cell suspensions (Groups of CNB‐TCS & FNA‐TCS), respectively, as comparison. The diagnostic efficacies, as well as the concordances of the FCM results with reference to the morphologic diagnoses were compared in these three groups. RESULTS: RT could yield sufficient cells for FCM immunophenotyping analysis, though a lower cell numbers compared to TCS technique. The diagnostic concordance was comparable in group CNB‐RT (91.1%) to the group CNB‐TCS (88.9%) and group FNA‐TCS (88.4%) (p = 0.819). The diagnostic sensitivity and specificity of CNB‐RT (91.1%; 100%) was not inferior to that of CNB‐TCS (88.9%; 100%) and FNA‐TCS (88.4%; 98.8%). CONCLUSIONS: This study shows the CNB‐RT presented non‐inferior diagnostic concordance and efficacy as compared to the TCS technique. CNB‐RT has the potential to produce cell suspension for FCM immunophenotyping while preserving tissue for lymphoma diagnosis and research. |
format | Online Article Text |
id | pubmed-7774716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77747162021-01-05 Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis Chi, Pei‐Dong Liu, Yi‐Jun Huang, Yu‐Hua Mao, Ming‐Jie Wang, Yu Li, Zhi‐Ming Li, Jian Cancer Med Clinical Cancer Research BACKGROUND: Conventional protocols utilize core needle biopsy (CNB) or fine needle aspiration (FNA) to produce cell suspension for flow cytometry (FCM) is a diagnostic challenge for lymphoid malignancies. We aim to develop an alternative CNB rinsing technique (RT) to produce cell suspension for FCM during this mini‐invasive procedure of CNB for lymphoma diagnosis. METHODS: FNA and CNB specimens from the same lesion of 93 patients with suspected lymphoma were collected under the guidance of B‐ultrasound simultaneously. The fresh CNB samples were prepared to cell suspension by RT for FCM immunophenotyping analysis (Group CNB‐RT). Then, the CNB tissues after performing the RT process and the fresh FNA tissues were processed by conventional tissue cell suspension (TCS) technique to obtain the cell suspensions (Groups of CNB‐TCS & FNA‐TCS), respectively, as comparison. The diagnostic efficacies, as well as the concordances of the FCM results with reference to the morphologic diagnoses were compared in these three groups. RESULTS: RT could yield sufficient cells for FCM immunophenotyping analysis, though a lower cell numbers compared to TCS technique. The diagnostic concordance was comparable in group CNB‐RT (91.1%) to the group CNB‐TCS (88.9%) and group FNA‐TCS (88.4%) (p = 0.819). The diagnostic sensitivity and specificity of CNB‐RT (91.1%; 100%) was not inferior to that of CNB‐TCS (88.9%; 100%) and FNA‐TCS (88.4%; 98.8%). CONCLUSIONS: This study shows the CNB‐RT presented non‐inferior diagnostic concordance and efficacy as compared to the TCS technique. CNB‐RT has the potential to produce cell suspension for FCM immunophenotyping while preserving tissue for lymphoma diagnosis and research. John Wiley and Sons Inc. 2020-10-17 /pmc/articles/PMC7774716/ /pubmed/33070470 http://dx.doi.org/10.1002/cam4.3540 Text en © 2020 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Cancer Research Chi, Pei‐Dong Liu, Yi‐Jun Huang, Yu‐Hua Mao, Ming‐Jie Wang, Yu Li, Zhi‐Ming Li, Jian Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis |
title | Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis |
title_full | Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis |
title_fullStr | Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis |
title_full_unstemmed | Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis |
title_short | Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis |
title_sort | rinsing sampling of core needle biopsy for flow cytometric analysis: a favorable method for lymphoma diagnosis |
topic | Clinical Cancer Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774716/ https://www.ncbi.nlm.nih.gov/pubmed/33070470 http://dx.doi.org/10.1002/cam4.3540 |
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