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Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt

OBJECTIVE: This study investigated the prevalence of Listeria monocytogenes in retail poultry shops, characterized the antibiotic resistance profile, and detected the genotypic pattern of virulence genes. MATERIALS AND METHODS: Broiler meat (n = 90), intestinal content (n = 40), and environmental sa...

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Autores principales: Aziz, Sahar Abdel Aleem Abdel, Mohamed, Manar Bahaa El Din
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774788/
https://www.ncbi.nlm.nih.gov/pubmed/33409317
http://dx.doi.org/10.5455/javar.2020.g472
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author Aziz, Sahar Abdel Aleem Abdel
Mohamed, Manar Bahaa El Din
author_facet Aziz, Sahar Abdel Aleem Abdel
Mohamed, Manar Bahaa El Din
author_sort Aziz, Sahar Abdel Aleem Abdel
collection PubMed
description OBJECTIVE: This study investigated the prevalence of Listeria monocytogenes in retail poultry shops, characterized the antibiotic resistance profile, and detected the genotypic pattern of virulence genes. MATERIALS AND METHODS: Broiler meat (n = 90), intestinal content (n = 40), and environmental samples (n = 95) were collected for this study. Besides, hand swabs (n = 20) were obtained from the poultry shop workers and stool samples (n = 40) were collected from the outpatient clinics of Beni-Suef University Hospital, Egypt. The samples were subjected to isolation and identification of L. monocytogenes by conventional bacteriological examinations and biochemical tests, followed by confirmatory identification by the polymerase chain reaction. RESULTS: Among the collected samples (n = 285), L. monocytogenes could be detected in 14.4% (n = 41/285) of the samples, where 30.0% (n = 12/40) of the intestinal content was positive. Similarly, 10.0% (n = 9/90), 15.0% (n = 3/20), and 12.5% (n = 5/40) of the samples of meat, hand swabs, and stools were found positive for L. monocytogenes, respectively. A total of 12 (12.6%) out of 95 environmental samples were positive for L. monocytogenes. Based on the antimicrobial sensitivity profile, most of the recovered isolates were multidrug-resistant against most commonly used antibiotics. CONCLUSION: The findings conclude that poultry shops play a vital role in transmitting L. monocytogenes to the consumers. Asymptomatic poultry shop workers should draw attention to their potentials for spreading the infection to the consumers through the contaminated carcasses. Low hygienic standards are present in commercial poultry shops that increase the risk of contamination in the sold products.
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spelling pubmed-77747882021-01-05 Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt Aziz, Sahar Abdel Aleem Abdel Mohamed, Manar Bahaa El Din J Adv Vet Anim Res Original Article OBJECTIVE: This study investigated the prevalence of Listeria monocytogenes in retail poultry shops, characterized the antibiotic resistance profile, and detected the genotypic pattern of virulence genes. MATERIALS AND METHODS: Broiler meat (n = 90), intestinal content (n = 40), and environmental samples (n = 95) were collected for this study. Besides, hand swabs (n = 20) were obtained from the poultry shop workers and stool samples (n = 40) were collected from the outpatient clinics of Beni-Suef University Hospital, Egypt. The samples were subjected to isolation and identification of L. monocytogenes by conventional bacteriological examinations and biochemical tests, followed by confirmatory identification by the polymerase chain reaction. RESULTS: Among the collected samples (n = 285), L. monocytogenes could be detected in 14.4% (n = 41/285) of the samples, where 30.0% (n = 12/40) of the intestinal content was positive. Similarly, 10.0% (n = 9/90), 15.0% (n = 3/20), and 12.5% (n = 5/40) of the samples of meat, hand swabs, and stools were found positive for L. monocytogenes, respectively. A total of 12 (12.6%) out of 95 environmental samples were positive for L. monocytogenes. Based on the antimicrobial sensitivity profile, most of the recovered isolates were multidrug-resistant against most commonly used antibiotics. CONCLUSION: The findings conclude that poultry shops play a vital role in transmitting L. monocytogenes to the consumers. Asymptomatic poultry shop workers should draw attention to their potentials for spreading the infection to the consumers through the contaminated carcasses. Low hygienic standards are present in commercial poultry shops that increase the risk of contamination in the sold products. A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2020-12-03 /pmc/articles/PMC7774788/ /pubmed/33409317 http://dx.doi.org/10.5455/javar.2020.g472 Text en Copyright: © Journal of Advanced Veterinary and Animal Research http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Aziz, Sahar Abdel Aleem Abdel
Mohamed, Manar Bahaa El Din
Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt
title Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt
title_full Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt
title_fullStr Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt
title_full_unstemmed Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt
title_short Prevalence, virulence genes, and antimicrobial resistance profile of Listeria monocytogenes isolated from retail poultry shops in Beni-Suef city, Egypt
title_sort prevalence, virulence genes, and antimicrobial resistance profile of listeria monocytogenes isolated from retail poultry shops in beni-suef city, egypt
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774788/
https://www.ncbi.nlm.nih.gov/pubmed/33409317
http://dx.doi.org/10.5455/javar.2020.g472
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