A primer for generating and using transcriptome data and gene sets

Transcriptomic approaches have provided a growing set of powerful tools with which to study genome-wide patterns of gene expression. Rapidly evolving technologies enable analysis of transcript abundance data from particular tissues and even single cells. This Primer discusses methods that can be use...

Descripción completa

Detalles Bibliográficos
Autores principales: Cockrum, Chad, Kaneshiro, Kiyomi R., Rechtsteiner, Andreas, Tabuchi, Tomoko M., Strome, Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2020
Materias:
Primer
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774889/
https://www.ncbi.nlm.nih.gov/pubmed/33361089
http://dx.doi.org/10.1242/dev.193854
_version_ 1783630355496960000
author Cockrum, Chad
Kaneshiro, Kiyomi R.
Rechtsteiner, Andreas
Tabuchi, Tomoko M.
Strome, Susan
author_facet Cockrum, Chad
Kaneshiro, Kiyomi R.
Rechtsteiner, Andreas
Tabuchi, Tomoko M.
Strome, Susan
author_sort Cockrum, Chad
collection PubMed
description Transcriptomic approaches have provided a growing set of powerful tools with which to study genome-wide patterns of gene expression. Rapidly evolving technologies enable analysis of transcript abundance data from particular tissues and even single cells. This Primer discusses methods that can be used to collect and profile RNAs from specific tissues or cells, process and analyze high-throughput RNA-sequencing data, and define sets of genes that accurately represent a category, such as tissue-enriched or tissue-specific gene expression.
format Online
Article
Text
id pubmed-7774889
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Company of Biologists Ltd
record_format MEDLINE/PubMed
spelling pubmed-77748892021-12-23 A primer for generating and using transcriptome data and gene sets Cockrum, Chad Kaneshiro, Kiyomi R. Rechtsteiner, Andreas Tabuchi, Tomoko M. Strome, Susan Development Primer Transcriptomic approaches have provided a growing set of powerful tools with which to study genome-wide patterns of gene expression. Rapidly evolving technologies enable analysis of transcript abundance data from particular tissues and even single cells. This Primer discusses methods that can be used to collect and profile RNAs from specific tissues or cells, process and analyze high-throughput RNA-sequencing data, and define sets of genes that accurately represent a category, such as tissue-enriched or tissue-specific gene expression. The Company of Biologists Ltd 2020-12-23 /pmc/articles/PMC7774889/ /pubmed/33361089 http://dx.doi.org/10.1242/dev.193854 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Primer
Cockrum, Chad
Kaneshiro, Kiyomi R.
Rechtsteiner, Andreas
Tabuchi, Tomoko M.
Strome, Susan
A primer for generating and using transcriptome data and gene sets
title A primer for generating and using transcriptome data and gene sets
title_full A primer for generating and using transcriptome data and gene sets
title_fullStr A primer for generating and using transcriptome data and gene sets
title_full_unstemmed A primer for generating and using transcriptome data and gene sets
title_short A primer for generating and using transcriptome data and gene sets
title_sort primer for generating and using transcriptome data and gene sets
topic Primer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774889/
https://www.ncbi.nlm.nih.gov/pubmed/33361089
http://dx.doi.org/10.1242/dev.193854
work_keys_str_mv AT cockrumchad aprimerforgeneratingandusingtranscriptomedataandgenesets
AT kaneshirokiyomir aprimerforgeneratingandusingtranscriptomedataandgenesets
AT rechtsteinerandreas aprimerforgeneratingandusingtranscriptomedataandgenesets
AT tabuchitomokom aprimerforgeneratingandusingtranscriptomedataandgenesets
AT stromesusan aprimerforgeneratingandusingtranscriptomedataandgenesets
AT cockrumchad primerforgeneratingandusingtranscriptomedataandgenesets
AT kaneshirokiyomir primerforgeneratingandusingtranscriptomedataandgenesets
AT rechtsteinerandreas primerforgeneratingandusingtranscriptomedataandgenesets
AT tabuchitomokom primerforgeneratingandusingtranscriptomedataandgenesets
AT stromesusan primerforgeneratingandusingtranscriptomedataandgenesets

Ejemplares similares