A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2

SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to dete...

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Autores principales: Maricic, Tomislav, Nickel, Olaf, Aximu-Petri, Ayinuer, Essel, Elena, Gansauge, Marie, Kanis, Philipp, Macak, Dominik, Richter, Julia, Riesenberg, Stephan, Bokelmann, Lukas, Zeberg, Hugo, Meyer, Matthias, Borte, Stephan, Pääbo, Svante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774962/
https://www.ncbi.nlm.nih.gov/pubmed/33382830
http://dx.doi.org/10.1371/journal.pone.0244824
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author Maricic, Tomislav
Nickel, Olaf
Aximu-Petri, Ayinuer
Essel, Elena
Gansauge, Marie
Kanis, Philipp
Macak, Dominik
Richter, Julia
Riesenberg, Stephan
Bokelmann, Lukas
Zeberg, Hugo
Meyer, Matthias
Borte, Stephan
Pääbo, Svante
author_facet Maricic, Tomislav
Nickel, Olaf
Aximu-Petri, Ayinuer
Essel, Elena
Gansauge, Marie
Kanis, Philipp
Macak, Dominik
Richter, Julia
Riesenberg, Stephan
Bokelmann, Lukas
Zeberg, Hugo
Meyer, Matthias
Borte, Stephan
Pääbo, Svante
author_sort Maricic, Tomislav
collection PubMed
description SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages (“mouthwashes”). This method detects individuals with large viral loads (Ct≤29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.
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spelling pubmed-77749622021-01-11 A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2 Maricic, Tomislav Nickel, Olaf Aximu-Petri, Ayinuer Essel, Elena Gansauge, Marie Kanis, Philipp Macak, Dominik Richter, Julia Riesenberg, Stephan Bokelmann, Lukas Zeberg, Hugo Meyer, Matthias Borte, Stephan Pääbo, Svante PLoS One Research Article SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages (“mouthwashes”). This method detects individuals with large viral loads (Ct≤29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics. Public Library of Science 2020-12-31 /pmc/articles/PMC7774962/ /pubmed/33382830 http://dx.doi.org/10.1371/journal.pone.0244824 Text en © 2020 Maricic et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Maricic, Tomislav
Nickel, Olaf
Aximu-Petri, Ayinuer
Essel, Elena
Gansauge, Marie
Kanis, Philipp
Macak, Dominik
Richter, Julia
Riesenberg, Stephan
Bokelmann, Lukas
Zeberg, Hugo
Meyer, Matthias
Borte, Stephan
Pääbo, Svante
A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
title A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
title_full A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
title_fullStr A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
title_full_unstemmed A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
title_short A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
title_sort direct rt-qpcr approach to test large numbers of individuals for sars-cov-2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774962/
https://www.ncbi.nlm.nih.gov/pubmed/33382830
http://dx.doi.org/10.1371/journal.pone.0244824
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