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Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies
Bats are among the most diverse, widespread, and abundant mammals. In Argentina, 67 species of bats have been recorded, belonging to 5 families and 29 genera. These high levels of biodiversity are likely to complicate identification at fieldwork, especially between closely related species, where ext...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775095/ https://www.ncbi.nlm.nih.gov/pubmed/33382800 http://dx.doi.org/10.1371/journal.pone.0244750 |
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author | Caraballo, Diego A. Montani, María E. Martínez, Leila M. Antoniazzi, Leandro R. Sambrana, Tomás C. Fernández, Camilo Cisterna, Daniel M. Beltrán, Fernando J. Colombo, Valeria C. |
author_facet | Caraballo, Diego A. Montani, María E. Martínez, Leila M. Antoniazzi, Leandro R. Sambrana, Tomás C. Fernández, Camilo Cisterna, Daniel M. Beltrán, Fernando J. Colombo, Valeria C. |
author_sort | Caraballo, Diego A. |
collection | PubMed |
description | Bats are among the most diverse, widespread, and abundant mammals. In Argentina, 67 species of bats have been recorded, belonging to 5 families and 29 genera. These high levels of biodiversity are likely to complicate identification at fieldwork, especially between closely related species, where external morphology-based approaches are the only immediate means for a priori species assignment. The use of molecular markers can enhance species identification, and acquires particular relevance in capture-release studies. In this study, we discuss the extent of the use of the mitochondrial cytochrome b gene for species identification, comparing external morphology identification with a molecular phylogenetic classification based on this marker, under the light of current bat systematics. We analyzed 33 samples collected in an eco-epidemiological survey in the province of Santa Fe (Argentina). We further sequenced 27 museum vouchers to test the accuracy of cytochrome b -based phylogenies in taxonomic identification of bats occurring in the Pampean/Chacoan regions of Argentina. The cytochrome b gene was successfully amplified in all Molossid and Vespertilionid species except for Eptesicus, for which we designed a new reverse primer. The resulting Bayesian phylogeny was congruent with current systematics. Cytochrome b proved useful for species-level delimitation in non-conflicting genera (Eumops, Dasypterus, Molossops) and has infrageneric resolution in more complex lineages (Eptesicus, Myotis, Molossus). We discuss four sources of incongruence that may act separately or in combination: 1) molecular processes, 2) biology, 3) limitations in identification, and 4) errors in the current taxonomy. The present study confirms the general applicability of cytochrome b -based phylogenies in eco-epidemiological studies, but its resolution and reliability depend mainly, but not solely, on the level of genetic differentiation within each bat genus. |
format | Online Article Text |
id | pubmed-7775095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77750952021-01-11 Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies Caraballo, Diego A. Montani, María E. Martínez, Leila M. Antoniazzi, Leandro R. Sambrana, Tomás C. Fernández, Camilo Cisterna, Daniel M. Beltrán, Fernando J. Colombo, Valeria C. PLoS One Research Article Bats are among the most diverse, widespread, and abundant mammals. In Argentina, 67 species of bats have been recorded, belonging to 5 families and 29 genera. These high levels of biodiversity are likely to complicate identification at fieldwork, especially between closely related species, where external morphology-based approaches are the only immediate means for a priori species assignment. The use of molecular markers can enhance species identification, and acquires particular relevance in capture-release studies. In this study, we discuss the extent of the use of the mitochondrial cytochrome b gene for species identification, comparing external morphology identification with a molecular phylogenetic classification based on this marker, under the light of current bat systematics. We analyzed 33 samples collected in an eco-epidemiological survey in the province of Santa Fe (Argentina). We further sequenced 27 museum vouchers to test the accuracy of cytochrome b -based phylogenies in taxonomic identification of bats occurring in the Pampean/Chacoan regions of Argentina. The cytochrome b gene was successfully amplified in all Molossid and Vespertilionid species except for Eptesicus, for which we designed a new reverse primer. The resulting Bayesian phylogeny was congruent with current systematics. Cytochrome b proved useful for species-level delimitation in non-conflicting genera (Eumops, Dasypterus, Molossops) and has infrageneric resolution in more complex lineages (Eptesicus, Myotis, Molossus). We discuss four sources of incongruence that may act separately or in combination: 1) molecular processes, 2) biology, 3) limitations in identification, and 4) errors in the current taxonomy. The present study confirms the general applicability of cytochrome b -based phylogenies in eco-epidemiological studies, but its resolution and reliability depend mainly, but not solely, on the level of genetic differentiation within each bat genus. Public Library of Science 2020-12-31 /pmc/articles/PMC7775095/ /pubmed/33382800 http://dx.doi.org/10.1371/journal.pone.0244750 Text en © 2020 Caraballo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Caraballo, Diego A. Montani, María E. Martínez, Leila M. Antoniazzi, Leandro R. Sambrana, Tomás C. Fernández, Camilo Cisterna, Daniel M. Beltrán, Fernando J. Colombo, Valeria C. Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies |
title | Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies |
title_full | Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies |
title_fullStr | Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies |
title_full_unstemmed | Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies |
title_short | Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies |
title_sort | heterogeneous taxonomic resolution of cytochrome b gene identification of bats from argentina: implications for field studies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775095/ https://www.ncbi.nlm.nih.gov/pubmed/33382800 http://dx.doi.org/10.1371/journal.pone.0244750 |
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