Cargando…

Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau

In ruminants, the bacterial community in the gastrointestinal tract (GIT) has an essential role in healthy growth. Examining the bacterial composition in the GIT between growth-retarded and normal yaks could improve our understanding of the role of microorganisms in yaks with growth retardation. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Jian, Zhu, Yixiao, Wang, Zhisheng, Yu, Xiong, Hu, Rui, Wang, Xueying, Cao, Guang, Zou, Huawei, Shah, Ali Mujtaba, Peng, Quanhui, Xue, Bai, Wang, Lizhi, Zhao, Suonan, Kong, Xiangying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775487/
https://www.ncbi.nlm.nih.gov/pubmed/33391217
http://dx.doi.org/10.3389/fmicb.2020.600516
_version_ 1783630477820690432
author Ma, Jian
Zhu, Yixiao
Wang, Zhisheng
Yu, Xiong
Hu, Rui
Wang, Xueying
Cao, Guang
Zou, Huawei
Shah, Ali Mujtaba
Peng, Quanhui
Xue, Bai
Wang, Lizhi
Zhao, Suonan
Kong, Xiangying
author_facet Ma, Jian
Zhu, Yixiao
Wang, Zhisheng
Yu, Xiong
Hu, Rui
Wang, Xueying
Cao, Guang
Zou, Huawei
Shah, Ali Mujtaba
Peng, Quanhui
Xue, Bai
Wang, Lizhi
Zhao, Suonan
Kong, Xiangying
author_sort Ma, Jian
collection PubMed
description In ruminants, the bacterial community in the gastrointestinal tract (GIT) has an essential role in healthy growth. Examining the bacterial composition in the GIT between growth-retarded and normal yaks could improve our understanding of the role of microorganisms in yaks with growth retardation. In this study, eight male yaks with growth retardation were used as the growth-retarded yak (GRY) group, and another eight male growth normal yaks (GNYs) with the same breed and age were used as the GNY group. We compared the bacterial community in the rumen, duodenum, jejunum, ileum, cecum, and colon between GRY and GNY groups based on the 16S ribosomal RNA gene sequencing. Alpha-diversity revealed that the Shannon index in the duodenum and ileum of the GNY group was higher (P < 0.05) than that of the GRY group. However, the opposite trend was found in the jejunum and cecum. The principal coordinates analysis (PCoA) showed that the bacterial structure in all segments of GIT differed from each other between two groups. In the rumen, the relative abundances of Ruminococcaceae NK4A214 group, Ruminococcaceae UCG-014, and Treponema 2 were higher (P < 0.05) in the GNY group as compared with the GRY group. However, the Christensenellaceae R-7 group exhibited an opposite trend. In the jejunum, compared with the GNY group, the unclassified Chitinophagaceae was enriched significantly (P < 0.05) in the GRY group. However, the unclassified Peptostreptococcaceae, Christensenellaceae R-7 group, and Lachnospiraceae NK3A20 group were enriched (P < 0.05) in the GNY group. In the ileum, the relative abundances of the Rikenellaceae RC9 gut group and Prevotellaceae UCG-004 were higher (P < 0.05) in the GNY group than those in the GRY group. In the cecum, the GNY group showed a higher (P < 0.05) relative abundance of Prevotellaceae UCG-003 as compared with the GRY group. In the colon, the relative abundances of Treponema 2 and unclassified Lachnospiraceae were slightly higher (0.05 < P < 0.10) in the GNY group than those in the GRY group. Overall, these results improve our knowledge about the bacterial composition in the GIT of growth-retarded and normal yaks, and regulating the bacterial community may be an effective solution to promote the compensatory growth of GRYs.
format Online
Article
Text
id pubmed-7775487
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77754872021-01-02 Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau Ma, Jian Zhu, Yixiao Wang, Zhisheng Yu, Xiong Hu, Rui Wang, Xueying Cao, Guang Zou, Huawei Shah, Ali Mujtaba Peng, Quanhui Xue, Bai Wang, Lizhi Zhao, Suonan Kong, Xiangying Front Microbiol Microbiology In ruminants, the bacterial community in the gastrointestinal tract (GIT) has an essential role in healthy growth. Examining the bacterial composition in the GIT between growth-retarded and normal yaks could improve our understanding of the role of microorganisms in yaks with growth retardation. In this study, eight male yaks with growth retardation were used as the growth-retarded yak (GRY) group, and another eight male growth normal yaks (GNYs) with the same breed and age were used as the GNY group. We compared the bacterial community in the rumen, duodenum, jejunum, ileum, cecum, and colon between GRY and GNY groups based on the 16S ribosomal RNA gene sequencing. Alpha-diversity revealed that the Shannon index in the duodenum and ileum of the GNY group was higher (P < 0.05) than that of the GRY group. However, the opposite trend was found in the jejunum and cecum. The principal coordinates analysis (PCoA) showed that the bacterial structure in all segments of GIT differed from each other between two groups. In the rumen, the relative abundances of Ruminococcaceae NK4A214 group, Ruminococcaceae UCG-014, and Treponema 2 were higher (P < 0.05) in the GNY group as compared with the GRY group. However, the Christensenellaceae R-7 group exhibited an opposite trend. In the jejunum, compared with the GNY group, the unclassified Chitinophagaceae was enriched significantly (P < 0.05) in the GRY group. However, the unclassified Peptostreptococcaceae, Christensenellaceae R-7 group, and Lachnospiraceae NK3A20 group were enriched (P < 0.05) in the GNY group. In the ileum, the relative abundances of the Rikenellaceae RC9 gut group and Prevotellaceae UCG-004 were higher (P < 0.05) in the GNY group than those in the GRY group. In the cecum, the GNY group showed a higher (P < 0.05) relative abundance of Prevotellaceae UCG-003 as compared with the GRY group. In the colon, the relative abundances of Treponema 2 and unclassified Lachnospiraceae were slightly higher (0.05 < P < 0.10) in the GNY group than those in the GRY group. Overall, these results improve our knowledge about the bacterial composition in the GIT of growth-retarded and normal yaks, and regulating the bacterial community may be an effective solution to promote the compensatory growth of GRYs. Frontiers Media S.A. 2020-12-18 /pmc/articles/PMC7775487/ /pubmed/33391217 http://dx.doi.org/10.3389/fmicb.2020.600516 Text en Copyright © 2020 Ma, Zhu, Wang, Yu, Hu, Wang, Cao, Zou, Shah, Peng, Xue, Wang, Zhao and Kong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ma, Jian
Zhu, Yixiao
Wang, Zhisheng
Yu, Xiong
Hu, Rui
Wang, Xueying
Cao, Guang
Zou, Huawei
Shah, Ali Mujtaba
Peng, Quanhui
Xue, Bai
Wang, Lizhi
Zhao, Suonan
Kong, Xiangying
Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau
title Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau
title_full Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau
title_fullStr Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau
title_full_unstemmed Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau
title_short Comparing the Bacterial Community in the Gastrointestinal Tracts Between Growth-Retarded and Normal Yaks on the Qinghai–Tibetan Plateau
title_sort comparing the bacterial community in the gastrointestinal tracts between growth-retarded and normal yaks on the qinghai–tibetan plateau
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775487/
https://www.ncbi.nlm.nih.gov/pubmed/33391217
http://dx.doi.org/10.3389/fmicb.2020.600516
work_keys_str_mv AT majian comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT zhuyixiao comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT wangzhisheng comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT yuxiong comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT hurui comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT wangxueying comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT caoguang comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT zouhuawei comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT shahalimujtaba comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT pengquanhui comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT xuebai comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT wanglizhi comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT zhaosuonan comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau
AT kongxiangying comparingthebacterialcommunityinthegastrointestinaltractsbetweengrowthretardedandnormalyaksontheqinghaitibetanplateau