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Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.

BACKGROUND AND AIM: Infections caused by Candida species are significantly increasing today, and invasive Candida infections are generally associated with high mortality. Early diagnosis and identification of Candida spp. is important for the determination of antifungal agents that will be used for...

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Autores principales: ALTINBAŞ, Rabiye, BARIŞ, Ayşe, ŞEN, Sümeyye, ÖZTÜRK, Recep, KİRAZ, Nuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775692/
https://www.ncbi.nlm.nih.gov/pubmed/32659879
http://dx.doi.org/10.3906/sag-1909-97
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author ALTINBAŞ, Rabiye
BARIŞ, Ayşe
ŞEN, Sümeyye
ÖZTÜRK, Recep
KİRAZ, Nuri
author_facet ALTINBAŞ, Rabiye
BARIŞ, Ayşe
ŞEN, Sümeyye
ÖZTÜRK, Recep
KİRAZ, Nuri
author_sort ALTINBAŞ, Rabiye
collection PubMed
description BACKGROUND AND AIM: Infections caused by Candida species are significantly increasing today, and invasive Candida infections are generally associated with high mortality. Early diagnosis and identification of Candida spp. is important for the determination of antifungal agents that will be used for treatment. The aim of the present study was to provide a better regimen for Candida infections in the future. MATERIALS AND METHODS: The Sensititre YeastOne (SYO) method was compared with The Clinical Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) testing method. Endpoints of minimal inhibitory concentrations (MICs) were determined for both methods. RESULTS: By using both methods, MIC values of micafungin, caspofungin, voriconazole, and fluconazole were lower than amphotericin B. The values obtained with the SYO method were in high categorical agreement for ecinocandins and amphotericin B. The results of voriconazole and fluconazole were in low categorical agreement. The categorical agreement between the SYO and the BMD results at 24 h was 82.1% for VORI and 98.4% for AMB. Values obtained with SYO method for all antifungal agents were in high essential agreement with the data of the CLSI reference BMD method. The essential agreement between the SYO and the BMD results at 24 h was 94.0% for MFG and 99.0% for AMB. CONCLUSIONS: The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates.
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spelling pubmed-77756922021-01-05 Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp. ALTINBAŞ, Rabiye BARIŞ, Ayşe ŞEN, Sümeyye ÖZTÜRK, Recep KİRAZ, Nuri Turk J Med Sci Article BACKGROUND AND AIM: Infections caused by Candida species are significantly increasing today, and invasive Candida infections are generally associated with high mortality. Early diagnosis and identification of Candida spp. is important for the determination of antifungal agents that will be used for treatment. The aim of the present study was to provide a better regimen for Candida infections in the future. MATERIALS AND METHODS: The Sensititre YeastOne (SYO) method was compared with The Clinical Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) testing method. Endpoints of minimal inhibitory concentrations (MICs) were determined for both methods. RESULTS: By using both methods, MIC values of micafungin, caspofungin, voriconazole, and fluconazole were lower than amphotericin B. The values obtained with the SYO method were in high categorical agreement for ecinocandins and amphotericin B. The results of voriconazole and fluconazole were in low categorical agreement. The categorical agreement between the SYO and the BMD results at 24 h was 82.1% for VORI and 98.4% for AMB. Values obtained with SYO method for all antifungal agents were in high essential agreement with the data of the CLSI reference BMD method. The essential agreement between the SYO and the BMD results at 24 h was 94.0% for MFG and 99.0% for AMB. CONCLUSIONS: The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates. The Scientific and Technological Research Council of Turkey 2020-12-17 /pmc/articles/PMC7775692/ /pubmed/32659879 http://dx.doi.org/10.3906/sag-1909-97 Text en Copyright © 2020 The Author(s) This article is distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
ALTINBAŞ, Rabiye
BARIŞ, Ayşe
ŞEN, Sümeyye
ÖZTÜRK, Recep
KİRAZ, Nuri
Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.
title Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.
title_full Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.
title_fullStr Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.
title_full_unstemmed Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.
title_short Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.
title_sort comparison of the sensititre yeastone antifungal method with the clsi m27-a3 reference method to determine the activity of antifungal agents against clinical isolates of candida spp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775692/
https://www.ncbi.nlm.nih.gov/pubmed/32659879
http://dx.doi.org/10.3906/sag-1909-97
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