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Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti
Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7776939/ https://www.ncbi.nlm.nih.gov/pubmed/33357054 http://dx.doi.org/10.1098/rstb.2019.0808 |
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author | Koskinioti, Panagiota Augustinos, Antonios A. Carvalho, Danilo O. Misbah-ul-Haq, Muhammad Pillwax, Gulizar de la Fuente, Lucia Duran Salvador-Herranz, Gustavo Herrero, Rafael Argilés Bourtzis, Kostas |
author_facet | Koskinioti, Panagiota Augustinos, Antonios A. Carvalho, Danilo O. Misbah-ul-Haq, Muhammad Pillwax, Gulizar de la Fuente, Lucia Duran Salvador-Herranz, Gustavo Herrero, Rafael Argilés Bourtzis, Kostas |
author_sort | Koskinioti, Panagiota |
collection | PubMed |
description | Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies. Here, we report on the construction of two genetic sexing strains using red- and white-eye colour mutations as selectable markers. Quality control analysis showed that the Red-eye genetic sexing strains (GSS) is better and more genetically stable than the White-eye GSS. The introduction of an irradiation-induced inversion (Inv35) increases genetic stability and reduces the probability of female contamination of the male release batches. Bi-weekly releases of irradiated males of both the Red-eye GSS and the Red-eye GSS/Inv35 fully suppressed target laboratory cage populations within six and nine weeks, respectively. An image analysis algorithm allowing sex determination based on eye colour identification at the pupal stage was developed. The next step is to automate the Red-eye-based genetic sexing and validate it in pilot trials prior to its integration in large-scale population suppression programmes. This article is part of the theme issue ‘Novel control strategies for mosquito-borne diseases’. |
format | Online Article Text |
id | pubmed-7776939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-77769392021-01-08 Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti Koskinioti, Panagiota Augustinos, Antonios A. Carvalho, Danilo O. Misbah-ul-Haq, Muhammad Pillwax, Gulizar de la Fuente, Lucia Duran Salvador-Herranz, Gustavo Herrero, Rafael Argilés Bourtzis, Kostas Philos Trans R Soc Lond B Biol Sci Articles Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies. Here, we report on the construction of two genetic sexing strains using red- and white-eye colour mutations as selectable markers. Quality control analysis showed that the Red-eye genetic sexing strains (GSS) is better and more genetically stable than the White-eye GSS. The introduction of an irradiation-induced inversion (Inv35) increases genetic stability and reduces the probability of female contamination of the male release batches. Bi-weekly releases of irradiated males of both the Red-eye GSS and the Red-eye GSS/Inv35 fully suppressed target laboratory cage populations within six and nine weeks, respectively. An image analysis algorithm allowing sex determination based on eye colour identification at the pupal stage was developed. The next step is to automate the Red-eye-based genetic sexing and validate it in pilot trials prior to its integration in large-scale population suppression programmes. This article is part of the theme issue ‘Novel control strategies for mosquito-borne diseases’. The Royal Society 2021-02-15 2020-12-28 /pmc/articles/PMC7776939/ /pubmed/33357054 http://dx.doi.org/10.1098/rstb.2019.0808 Text en © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Articles Koskinioti, Panagiota Augustinos, Antonios A. Carvalho, Danilo O. Misbah-ul-Haq, Muhammad Pillwax, Gulizar de la Fuente, Lucia Duran Salvador-Herranz, Gustavo Herrero, Rafael Argilés Bourtzis, Kostas Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti |
title | Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti |
title_full | Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti |
title_fullStr | Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti |
title_full_unstemmed | Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti |
title_short | Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti |
title_sort | genetic sexing strains for the population suppression of the mosquito vector aedes aegypti |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7776939/ https://www.ncbi.nlm.nih.gov/pubmed/33357054 http://dx.doi.org/10.1098/rstb.2019.0808 |
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