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Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti

Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies...

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Detalles Bibliográficos
Autores principales: Koskinioti, Panagiota, Augustinos, Antonios A., Carvalho, Danilo O., Misbah-ul-Haq, Muhammad, Pillwax, Gulizar, de la Fuente, Lucia Duran, Salvador-Herranz, Gustavo, Herrero, Rafael Argilés, Bourtzis, Kostas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7776939/
https://www.ncbi.nlm.nih.gov/pubmed/33357054
http://dx.doi.org/10.1098/rstb.2019.0808
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author Koskinioti, Panagiota
Augustinos, Antonios A.
Carvalho, Danilo O.
Misbah-ul-Haq, Muhammad
Pillwax, Gulizar
de la Fuente, Lucia Duran
Salvador-Herranz, Gustavo
Herrero, Rafael Argilés
Bourtzis, Kostas
author_facet Koskinioti, Panagiota
Augustinos, Antonios A.
Carvalho, Danilo O.
Misbah-ul-Haq, Muhammad
Pillwax, Gulizar
de la Fuente, Lucia Duran
Salvador-Herranz, Gustavo
Herrero, Rafael Argilés
Bourtzis, Kostas
author_sort Koskinioti, Panagiota
collection PubMed
description Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies. Here, we report on the construction of two genetic sexing strains using red- and white-eye colour mutations as selectable markers. Quality control analysis showed that the Red-eye genetic sexing strains (GSS) is better and more genetically stable than the White-eye GSS. The introduction of an irradiation-induced inversion (Inv35) increases genetic stability and reduces the probability of female contamination of the male release batches. Bi-weekly releases of irradiated males of both the Red-eye GSS and the Red-eye GSS/Inv35 fully suppressed target laboratory cage populations within six and nine weeks, respectively. An image analysis algorithm allowing sex determination based on eye colour identification at the pupal stage was developed. The next step is to automate the Red-eye-based genetic sexing and validate it in pilot trials prior to its integration in large-scale population suppression programmes. This article is part of the theme issue ‘Novel control strategies for mosquito-borne diseases’.
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spelling pubmed-77769392021-01-08 Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti Koskinioti, Panagiota Augustinos, Antonios A. Carvalho, Danilo O. Misbah-ul-Haq, Muhammad Pillwax, Gulizar de la Fuente, Lucia Duran Salvador-Herranz, Gustavo Herrero, Rafael Argilés Bourtzis, Kostas Philos Trans R Soc Lond B Biol Sci Articles Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies. Here, we report on the construction of two genetic sexing strains using red- and white-eye colour mutations as selectable markers. Quality control analysis showed that the Red-eye genetic sexing strains (GSS) is better and more genetically stable than the White-eye GSS. The introduction of an irradiation-induced inversion (Inv35) increases genetic stability and reduces the probability of female contamination of the male release batches. Bi-weekly releases of irradiated males of both the Red-eye GSS and the Red-eye GSS/Inv35 fully suppressed target laboratory cage populations within six and nine weeks, respectively. An image analysis algorithm allowing sex determination based on eye colour identification at the pupal stage was developed. The next step is to automate the Red-eye-based genetic sexing and validate it in pilot trials prior to its integration in large-scale population suppression programmes. This article is part of the theme issue ‘Novel control strategies for mosquito-borne diseases’. The Royal Society 2021-02-15 2020-12-28 /pmc/articles/PMC7776939/ /pubmed/33357054 http://dx.doi.org/10.1098/rstb.2019.0808 Text en © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Articles
Koskinioti, Panagiota
Augustinos, Antonios A.
Carvalho, Danilo O.
Misbah-ul-Haq, Muhammad
Pillwax, Gulizar
de la Fuente, Lucia Duran
Salvador-Herranz, Gustavo
Herrero, Rafael Argilés
Bourtzis, Kostas
Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti
title Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti
title_full Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti
title_fullStr Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti
title_full_unstemmed Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti
title_short Genetic sexing strains for the population suppression of the mosquito vector Aedes aegypti
title_sort genetic sexing strains for the population suppression of the mosquito vector aedes aegypti
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7776939/
https://www.ncbi.nlm.nih.gov/pubmed/33357054
http://dx.doi.org/10.1098/rstb.2019.0808
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