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490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators

BACKGROUND: During the Coronavirus Disease 2019 pandemic, shortages of personal protective equipment (PPE) have forced many healthcare facilities to require personnel to reuse N95 respirators. We hypothesized that use of correct technique such as changing gloves after N95 contact or providing rapid...

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Autores principales: Li, Daniel, Alhmidi, Heba, Pearlmutter, Basya, Cadnum, Jennifer, Wilson, Brigid, Donskey, Curtis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7777997/
http://dx.doi.org/10.1093/ofid/ofaa439.683
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author Li, Daniel
Alhmidi, Heba
Pearlmutter, Basya
Cadnum, Jennifer
Wilson, Brigid
Donskey, Curtis
author_facet Li, Daniel
Alhmidi, Heba
Pearlmutter, Basya
Cadnum, Jennifer
Wilson, Brigid
Donskey, Curtis
author_sort Li, Daniel
collection PubMed
description BACKGROUND: During the Coronavirus Disease 2019 pandemic, shortages of personal protective equipment (PPE) have forced many healthcare facilities to require personnel to reuse N95 respirators. We hypothesized that use of correct technique such as changing gloves after N95 contact or providing rapid decontamination between each use would reduce the risk for contamination. METHODS: We conducted a randomized crossover study using simulated patient care interactions to compare the effectiveness of interventions to reduce contamination of personnel and the environment with high-level N95 contamination (10(7) plaque-forming units [PFU] of bacteriophage MS2 applied to front of respirator). Ten healthcare personnel performed 4 randomly-assigned standardized examinations of mannequins including: 1) Control (PPE donning and doffing not including glove change after N95 contact); 2) Glove change after any N95 contact; 3) Control with 1-minute ultraviolet-C light (UV-C) treatment between simulations; 4) Control with 30-second steam treatment between simulations. A second trial was conducted with groups 1–3 using a lower inoculum (10(5) PFU). The frequencies of participant and environmental contamination were compared. RESULTS: As shown in Figure 1.A, use of a highly contaminated N95 respirator resulted in frequent MS2 contamination in the Control, Glove change, and UV-C groups, but was dramatically reduced with steam treatment of the N95. With the lower level of contamination, MS2 contamination occurred less frequently across all groups, and was significantly reduced in the UV group, compared to the Control (Figure 1.B). Figure 1: Bacteriophage MS2 contamination during simulated patient care interactions with contaminated N95 respirators [Image: see text] CONCLUSION: Reuse of contaminated N95 respirators resulted in contamination of personnel and the environment even when correct technique was used. Rapid decontamination technologies can reduce the risk for transmission. DISCLOSURES: All Authors: No reported disclosures
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spelling pubmed-77779972021-01-07 490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators Li, Daniel Alhmidi, Heba Pearlmutter, Basya Cadnum, Jennifer Wilson, Brigid Donskey, Curtis Open Forum Infect Dis Poster Abstracts BACKGROUND: During the Coronavirus Disease 2019 pandemic, shortages of personal protective equipment (PPE) have forced many healthcare facilities to require personnel to reuse N95 respirators. We hypothesized that use of correct technique such as changing gloves after N95 contact or providing rapid decontamination between each use would reduce the risk for contamination. METHODS: We conducted a randomized crossover study using simulated patient care interactions to compare the effectiveness of interventions to reduce contamination of personnel and the environment with high-level N95 contamination (10(7) plaque-forming units [PFU] of bacteriophage MS2 applied to front of respirator). Ten healthcare personnel performed 4 randomly-assigned standardized examinations of mannequins including: 1) Control (PPE donning and doffing not including glove change after N95 contact); 2) Glove change after any N95 contact; 3) Control with 1-minute ultraviolet-C light (UV-C) treatment between simulations; 4) Control with 30-second steam treatment between simulations. A second trial was conducted with groups 1–3 using a lower inoculum (10(5) PFU). The frequencies of participant and environmental contamination were compared. RESULTS: As shown in Figure 1.A, use of a highly contaminated N95 respirator resulted in frequent MS2 contamination in the Control, Glove change, and UV-C groups, but was dramatically reduced with steam treatment of the N95. With the lower level of contamination, MS2 contamination occurred less frequently across all groups, and was significantly reduced in the UV group, compared to the Control (Figure 1.B). Figure 1: Bacteriophage MS2 contamination during simulated patient care interactions with contaminated N95 respirators [Image: see text] CONCLUSION: Reuse of contaminated N95 respirators resulted in contamination of personnel and the environment even when correct technique was used. Rapid decontamination technologies can reduce the risk for transmission. DISCLOSURES: All Authors: No reported disclosures Oxford University Press 2020-12-31 /pmc/articles/PMC7777997/ http://dx.doi.org/10.1093/ofid/ofaa439.683 Text en © The Author 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Poster Abstracts
Li, Daniel
Alhmidi, Heba
Pearlmutter, Basya
Cadnum, Jennifer
Wilson, Brigid
Donskey, Curtis
490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators
title 490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators
title_full 490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators
title_fullStr 490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators
title_full_unstemmed 490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators
title_short 490. A Randomized Crossover Study to Evaluate Interventions to Reduce Contamination during Reuse of N95 Respirators
title_sort 490. a randomized crossover study to evaluate interventions to reduce contamination during reuse of n95 respirators
topic Poster Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7777997/
http://dx.doi.org/10.1093/ofid/ofaa439.683
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