Cargando…

726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients

BACKGROUND: New generation multiplex polymerase chain reaction (PCR) panels have led to the ability of rapid detection of Enteropathogenic Escherichia coli (EPEC). Although many studies have looked at the pathogenesis of this organism in the pediatric populations, fewer studies include adults. We ai...

Descripción completa

Detalles Bibliográficos
Autores principales: Lusby, Heather, Rand, Kenneth, Beal, Stacy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778154/
http://dx.doi.org/10.1093/ofid/ofaa439.918
_version_ 1783631070668783616
author Lusby, Heather
Rand, Kenneth
Beal, Stacy
author_facet Lusby, Heather
Rand, Kenneth
Beal, Stacy
author_sort Lusby, Heather
collection PubMed
description BACKGROUND: New generation multiplex polymerase chain reaction (PCR) panels have led to the ability of rapid detection of Enteropathogenic Escherichia coli (EPEC). Although many studies have looked at the pathogenesis of this organism in the pediatric populations, fewer studies include adults. We aimed to determine if EPEC is pathogenic on its own, or has a predilection for certain populations based upon risk factors. This was achieved by comparing risk factors in patients positive for EPEC on the BioFire Gastrointestinal (GI) Pathogen Panel versus patients negative for all targets on the panel. METHODS: This is a single center case control study that was performed using a retrospective chart review from January 1, 2016 thru August 31, 2019. All patients were symptomatic with diarrheal illness and had a GI pathogen panel performed. The study group were patients that were EPEC positive. The control group were patients with a negative PCR panel that were matched three to one based upon age and gender. Chi-squared statistical analysis was used. RESULTS: 792 patients were evaluated. In the adult group the EPEC positive actual length of stay (LOS) was 13.5 rather than 9.3 in the control. Although this was not statically significant this trended towards a longer LOS as seen in Table 1. In the pediatric population, both expected and actual LOS were statically longer viewed in Table 2. This may be due to these patients being more chronically ill at baseline, with a greater number of pediatric coronary artery disease and ulcerative colitis in the control. Males were more likely to test positive for EPEC at 69.86% in the pediatric population than in the adult population with statistical significance at p=0.0035. The pediatric population had more co-infections with EPEC at 57.53% with p< 0.0001. This can be further seen in Table 3. Table 1 [Image: see text] Table 2 [Image: see text] Table 3 [Image: see text] CONCLUSION: In the adult EPEC positive patients there was no statistically significant difference in length of stay in comparison to negative control. This raises the question to if this is truly a pathogen in adults. In the pediatric population, our control group was likely more ill, which made it difficult interpret the significance of differences in LOS. DISCLOSURES: Kenneth Rand, MD, BioFire Diagnostics, Inc (Consultant, Grant/Research Support, Advisor or Review Panel member, Research Grant or Support) Stacy Beal, MD, FilmArray BioFire (Grant/Research Support)
format Online
Article
Text
id pubmed-7778154
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-77781542021-01-07 726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients Lusby, Heather Rand, Kenneth Beal, Stacy Open Forum Infect Dis Poster Abstracts BACKGROUND: New generation multiplex polymerase chain reaction (PCR) panels have led to the ability of rapid detection of Enteropathogenic Escherichia coli (EPEC). Although many studies have looked at the pathogenesis of this organism in the pediatric populations, fewer studies include adults. We aimed to determine if EPEC is pathogenic on its own, or has a predilection for certain populations based upon risk factors. This was achieved by comparing risk factors in patients positive for EPEC on the BioFire Gastrointestinal (GI) Pathogen Panel versus patients negative for all targets on the panel. METHODS: This is a single center case control study that was performed using a retrospective chart review from January 1, 2016 thru August 31, 2019. All patients were symptomatic with diarrheal illness and had a GI pathogen panel performed. The study group were patients that were EPEC positive. The control group were patients with a negative PCR panel that were matched three to one based upon age and gender. Chi-squared statistical analysis was used. RESULTS: 792 patients were evaluated. In the adult group the EPEC positive actual length of stay (LOS) was 13.5 rather than 9.3 in the control. Although this was not statically significant this trended towards a longer LOS as seen in Table 1. In the pediatric population, both expected and actual LOS were statically longer viewed in Table 2. This may be due to these patients being more chronically ill at baseline, with a greater number of pediatric coronary artery disease and ulcerative colitis in the control. Males were more likely to test positive for EPEC at 69.86% in the pediatric population than in the adult population with statistical significance at p=0.0035. The pediatric population had more co-infections with EPEC at 57.53% with p< 0.0001. This can be further seen in Table 3. Table 1 [Image: see text] Table 2 [Image: see text] Table 3 [Image: see text] CONCLUSION: In the adult EPEC positive patients there was no statistically significant difference in length of stay in comparison to negative control. This raises the question to if this is truly a pathogen in adults. In the pediatric population, our control group was likely more ill, which made it difficult interpret the significance of differences in LOS. DISCLOSURES: Kenneth Rand, MD, BioFire Diagnostics, Inc (Consultant, Grant/Research Support, Advisor or Review Panel member, Research Grant or Support) Stacy Beal, MD, FilmArray BioFire (Grant/Research Support) Oxford University Press 2020-12-31 /pmc/articles/PMC7778154/ http://dx.doi.org/10.1093/ofid/ofaa439.918 Text en © The Author 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Poster Abstracts
Lusby, Heather
Rand, Kenneth
Beal, Stacy
726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients
title 726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients
title_full 726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients
title_fullStr 726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients
title_full_unstemmed 726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients
title_short 726. Investigation of Epidemiological Characteristics and Outcomes in Enteropathogenic Escherichia coli positive patients
title_sort 726. investigation of epidemiological characteristics and outcomes in enteropathogenic escherichia coli positive patients
topic Poster Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778154/
http://dx.doi.org/10.1093/ofid/ofaa439.918
work_keys_str_mv AT lusbyheather 726investigationofepidemiologicalcharacteristicsandoutcomesinenteropathogenicescherichiacolipositivepatients
AT randkenneth 726investigationofepidemiologicalcharacteristicsandoutcomesinenteropathogenicescherichiacolipositivepatients
AT bealstacy 726investigationofepidemiologicalcharacteristicsandoutcomesinenteropathogenicescherichiacolipositivepatients