Cargando…

Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria

BACKGROUND: Increasing antibiotic resistance and the emergence of multidrug-resistant (MDR) pathogens have led to the need to develop new therapeutic agents to tackle microbial infections. Nano-antibiotics are a novel generation of nanomaterials with significant antimicrobial activities that target...

Descripción completa

Detalles Bibliográficos
Autores principales: Hamida, Reham Samir, Ali, Mohamed Abdelaal, Goda, Doaa A, Al-Zaban, Mayasar Ibrahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778443/
https://www.ncbi.nlm.nih.gov/pubmed/33402822
http://dx.doi.org/10.2147/IJN.S289243
_version_ 1783631129376456704
author Hamida, Reham Samir
Ali, Mohamed Abdelaal
Goda, Doaa A
Al-Zaban, Mayasar Ibrahim
author_facet Hamida, Reham Samir
Ali, Mohamed Abdelaal
Goda, Doaa A
Al-Zaban, Mayasar Ibrahim
author_sort Hamida, Reham Samir
collection PubMed
description BACKGROUND: Increasing antibiotic resistance and the emergence of multidrug-resistant (MDR) pathogens have led to the need to develop new therapeutic agents to tackle microbial infections. Nano-antibiotics are a novel generation of nanomaterials with significant antimicrobial activities that target bacterial defense systems including biofilm formation, membrane permeability, and virulence activity. PURPOSE: In addition to AgNO(3,) the current study aimed to explore for first time the antibacterial potential of silver nanoparticles synthesized by Nostoc sp. Bahar_M (N-SNPs) and their killing mechanisms against Streptococcus mutans, methicillin-resistant Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium. METHODS: Potential mechanisms of action of both silver species against bacteria were systematically explored using agar well diffusion, enzyme (lactate dehydrogenase (LDH) and ATPase) and antioxidant (glutathione peroxidase and catalase)   assays, and morphological examinations. qRT-PCR and SDS-PAGE were employed to investigate the effect of both treatments on mfD, flu, and hly gene expression and protein patterns, respectively. RESULTS: N-SNPs exhibited greater biocidal activity than AgNO(3) against the four tested bacteria. E. coli treated with N-SNPs showed significant surges in LDH levels, imbalances in other antioxidant and enzyme activities, and marked morphological changes, including cell membrane disruption and cytoplasmic dissolution. N-SNPs caused more significant upregulation of mfD expression and downregulation of both flu and hly expression and increased protein denaturation compared with AgNO(3). CONCLUSION: N-SNPs exhibited significant inhibitory potential against E. coli by direct interfering with bacterial cellular structures and/or enhancing oxidative stress, indicating their potential for use as an alternative antimicrobial agent. However, the potential of N-SNPs to be usable and biocompatible antibacterial drug will evaluate by their toxicity against normal cells.
format Online
Article
Text
id pubmed-7778443
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-77784432021-01-04 Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria Hamida, Reham Samir Ali, Mohamed Abdelaal Goda, Doaa A Al-Zaban, Mayasar Ibrahim Int J Nanomedicine Original Research BACKGROUND: Increasing antibiotic resistance and the emergence of multidrug-resistant (MDR) pathogens have led to the need to develop new therapeutic agents to tackle microbial infections. Nano-antibiotics are a novel generation of nanomaterials with significant antimicrobial activities that target bacterial defense systems including biofilm formation, membrane permeability, and virulence activity. PURPOSE: In addition to AgNO(3,) the current study aimed to explore for first time the antibacterial potential of silver nanoparticles synthesized by Nostoc sp. Bahar_M (N-SNPs) and their killing mechanisms against Streptococcus mutans, methicillin-resistant Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium. METHODS: Potential mechanisms of action of both silver species against bacteria were systematically explored using agar well diffusion, enzyme (lactate dehydrogenase (LDH) and ATPase) and antioxidant (glutathione peroxidase and catalase)   assays, and morphological examinations. qRT-PCR and SDS-PAGE were employed to investigate the effect of both treatments on mfD, flu, and hly gene expression and protein patterns, respectively. RESULTS: N-SNPs exhibited greater biocidal activity than AgNO(3) against the four tested bacteria. E. coli treated with N-SNPs showed significant surges in LDH levels, imbalances in other antioxidant and enzyme activities, and marked morphological changes, including cell membrane disruption and cytoplasmic dissolution. N-SNPs caused more significant upregulation of mfD expression and downregulation of both flu and hly expression and increased protein denaturation compared with AgNO(3). CONCLUSION: N-SNPs exhibited significant inhibitory potential against E. coli by direct interfering with bacterial cellular structures and/or enhancing oxidative stress, indicating their potential for use as an alternative antimicrobial agent. However, the potential of N-SNPs to be usable and biocompatible antibacterial drug will evaluate by their toxicity against normal cells. Dove 2020-12-29 /pmc/articles/PMC7778443/ /pubmed/33402822 http://dx.doi.org/10.2147/IJN.S289243 Text en © 2020 Hamida et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Hamida, Reham Samir
Ali, Mohamed Abdelaal
Goda, Doaa A
Al-Zaban, Mayasar Ibrahim
Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_full Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_fullStr Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_full_unstemmed Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_short Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_sort lethal mechanisms of nostoc-synthesized silver nanoparticles against different pathogenic bacteria
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778443/
https://www.ncbi.nlm.nih.gov/pubmed/33402822
http://dx.doi.org/10.2147/IJN.S289243
work_keys_str_mv AT hamidarehamsamir lethalmechanismsofnostocsynthesizedsilvernanoparticlesagainstdifferentpathogenicbacteria
AT alimohamedabdelaal lethalmechanismsofnostocsynthesizedsilvernanoparticlesagainstdifferentpathogenicbacteria
AT godadoaaa lethalmechanismsofnostocsynthesizedsilvernanoparticlesagainstdifferentpathogenicbacteria
AT alzabanmayasaribrahim lethalmechanismsofnostocsynthesizedsilvernanoparticlesagainstdifferentpathogenicbacteria