Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China

To investigate the viral communities in diarrhoeal faeces of Tibetan pigs, 146 diarrhoeic samples were collected from 16 pigs farms on the Tibetan plateau. Nineteen viruses belonging to eleven viral taxonomic families were identified in a pooled library. Metagenomics analysis revealed that the virus...

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Autores principales: Zhou, Long, Hu, Chengzhe, Zhou, Qun, Yang, Danjiao, Wang, Lixuan, Zhang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778721/
https://www.ncbi.nlm.nih.gov/pubmed/33389103
http://dx.doi.org/10.1007/s00705-020-04932-9
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author Zhou, Long
Hu, Chengzhe
Zhou, Qun
Yang, Danjiao
Wang, Lixuan
Zhang, Bin
author_facet Zhou, Long
Hu, Chengzhe
Zhou, Qun
Yang, Danjiao
Wang, Lixuan
Zhang, Bin
author_sort Zhou, Long
collection PubMed
description To investigate the viral communities in diarrhoeal faeces of Tibetan pigs, 146 diarrhoeic samples were collected from 16 pigs farms on the Tibetan plateau. Nineteen viruses belonging to eleven viral taxonomic families were identified in a pooled library. Metagenomics analysis revealed that the viruses were mainly small linear and circular DNA viruses. Furthermore, sequences of 10 NS1 genes and two complete genomes of PBuVs were obtained by PCR amplification. Sequence comparisons and phylogenetic analysis showed that the PBuVs from Tibetan pigs displayed more abundant genetic diversity than those from domestic pigs. This is the first description of the faecal viral community in Tibetan pigs associated with diarrhoea. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-020-04932-9.
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spelling pubmed-77787212021-01-04 Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China Zhou, Long Hu, Chengzhe Zhou, Qun Yang, Danjiao Wang, Lixuan Zhang, Bin Arch Virol Brief Report To investigate the viral communities in diarrhoeal faeces of Tibetan pigs, 146 diarrhoeic samples were collected from 16 pigs farms on the Tibetan plateau. Nineteen viruses belonging to eleven viral taxonomic families were identified in a pooled library. Metagenomics analysis revealed that the viruses were mainly small linear and circular DNA viruses. Furthermore, sequences of 10 NS1 genes and two complete genomes of PBuVs were obtained by PCR amplification. Sequence comparisons and phylogenetic analysis showed that the PBuVs from Tibetan pigs displayed more abundant genetic diversity than those from domestic pigs. This is the first description of the faecal viral community in Tibetan pigs associated with diarrhoea. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-020-04932-9. Springer Vienna 2021-01-03 2021 /pmc/articles/PMC7778721/ /pubmed/33389103 http://dx.doi.org/10.1007/s00705-020-04932-9 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH, AT part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Zhou, Long
Hu, Chengzhe
Zhou, Qun
Yang, Danjiao
Wang, Lixuan
Zhang, Bin
Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China
title Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China
title_full Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China
title_fullStr Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China
title_full_unstemmed Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China
title_short Viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in Tibetan pigs in China
title_sort viral communities associated with porcine diarrhoeal disease and genetic characterization of a bufavirus in tibetan pigs in china
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778721/
https://www.ncbi.nlm.nih.gov/pubmed/33389103
http://dx.doi.org/10.1007/s00705-020-04932-9
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