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Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study

BACKGROUND: The spermatogenesis system includes highly radiosensitive cells. Hence, this system is a potential target for toxic effects of ionizing radiation during radiotherapy of abdomen and pelvis cancers, as well as after accidental radiation events. Accordingly, metformin and melatonin are two...

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Autores principales: Tajabadi, Elham, Javadi, Abdolreza, Azar, Nasim Ahmadi, Najafi, Masoud, Shirazi, Alireza, Shabeeb, Dheyauldeen, Musa, Ahmed Eleojo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Knowledge E 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778753/
https://www.ncbi.nlm.nih.gov/pubmed/33426418
http://dx.doi.org/10.18502/ijrm.v18i12.8029
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author Tajabadi, Elham
Javadi, Abdolreza
Azar, Nasim Ahmadi
Najafi, Masoud
Shirazi, Alireza
Shabeeb, Dheyauldeen
Musa, Ahmed Eleojo
author_facet Tajabadi, Elham
Javadi, Abdolreza
Azar, Nasim Ahmadi
Najafi, Masoud
Shirazi, Alireza
Shabeeb, Dheyauldeen
Musa, Ahmed Eleojo
author_sort Tajabadi, Elham
collection PubMed
description BACKGROUND: The spermatogenesis system includes highly radiosensitive cells. Hence, this system is a potential target for toxic effects of ionizing radiation during radiotherapy of abdomen and pelvis cancers, as well as after accidental radiation events. Accordingly, metformin and melatonin are two important radioprotectors that have shown an ability to prevent cell death through neutralization of free radicals and stimulating DNA damage responses. OBJECTIVE: To evaluate the radioprotective effects of melatonin and metformin on mice spermatogenesis when administered alone or as a combination. MATERIALS AND METHODS: In this histological Study, 40 (6-8 wk, 30 gr) NMRI mice were divided into 8 groups (n = 5/each) as control, metformin, melatonin, melatonin + metformin, radiation, radiation + melatonin, radiation + metformin, and radiation + melatonin + metformin. 37 days after the irradiation, the testicular tissues were collected for histological evaluation. RESULTS: Single administration of melatonin could ameliorate effectively radiation toxicity in mice testis. Metformin showed radioprotective effects on some parameters such as the numbers of spermatogonia and mature sperms. Interestingly, the melatonin and metformin combination reversed the reduced number of sperms rather than single drug administration. CONCLUSION: The combination of melatonin with metformin can protect mice spermatogenesis against ionizing radiation more effectively compared to the single forms of these drugs.
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spelling pubmed-77787532021-01-08 Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study Tajabadi, Elham Javadi, Abdolreza Azar, Nasim Ahmadi Najafi, Masoud Shirazi, Alireza Shabeeb, Dheyauldeen Musa, Ahmed Eleojo Int J Reprod Biomed Research Article BACKGROUND: The spermatogenesis system includes highly radiosensitive cells. Hence, this system is a potential target for toxic effects of ionizing radiation during radiotherapy of abdomen and pelvis cancers, as well as after accidental radiation events. Accordingly, metformin and melatonin are two important radioprotectors that have shown an ability to prevent cell death through neutralization of free radicals and stimulating DNA damage responses. OBJECTIVE: To evaluate the radioprotective effects of melatonin and metformin on mice spermatogenesis when administered alone or as a combination. MATERIALS AND METHODS: In this histological Study, 40 (6-8 wk, 30 gr) NMRI mice were divided into 8 groups (n = 5/each) as control, metformin, melatonin, melatonin + metformin, radiation, radiation + melatonin, radiation + metformin, and radiation + melatonin + metformin. 37 days after the irradiation, the testicular tissues were collected for histological evaluation. RESULTS: Single administration of melatonin could ameliorate effectively radiation toxicity in mice testis. Metformin showed radioprotective effects on some parameters such as the numbers of spermatogonia and mature sperms. Interestingly, the melatonin and metformin combination reversed the reduced number of sperms rather than single drug administration. CONCLUSION: The combination of melatonin with metformin can protect mice spermatogenesis against ionizing radiation more effectively compared to the single forms of these drugs. Knowledge E 2020-12-21 /pmc/articles/PMC7778753/ /pubmed/33426418 http://dx.doi.org/10.18502/ijrm.v18i12.8029 Text en Copyright © 2020 Tajabadi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Article
Tajabadi, Elham
Javadi, Abdolreza
Azar, Nasim Ahmadi
Najafi, Masoud
Shirazi, Alireza
Shabeeb, Dheyauldeen
Musa, Ahmed Eleojo
Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study
title Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study
title_full Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study
title_fullStr Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study
title_full_unstemmed Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study
title_short Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study
title_sort radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: a histological study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778753/
https://www.ncbi.nlm.nih.gov/pubmed/33426418
http://dx.doi.org/10.18502/ijrm.v18i12.8029
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