Cargando…
The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study
BACKGROUND: To increase the results of infertility treatment, many efforts have been made to improve the treatment methods. As assisted reproductive technology is mainly using cell culture methods, one of the approaches to improve this technology is conditioned medium from different sources. It is d...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Knowledge E
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778757/ https://www.ncbi.nlm.nih.gov/pubmed/33426412 http://dx.doi.org/10.18502/ijrm.v18i12.8023 |
_version_ | 1783631188249804800 |
---|---|
author | Adib, Maryam Seifati, Seyed Morteza Ashkezari, Mahmood Dehghani Khoradmehr, Arezoo Rezaee-Ranjbar-Sardari, Roshan Tahajjodi, Somayyeh Sadat Aflatoonian, Behrouz |
author_facet | Adib, Maryam Seifati, Seyed Morteza Ashkezari, Mahmood Dehghani Khoradmehr, Arezoo Rezaee-Ranjbar-Sardari, Roshan Tahajjodi, Somayyeh Sadat Aflatoonian, Behrouz |
author_sort | Adib, Maryam |
collection | PubMed |
description | BACKGROUND: To increase the results of infertility treatment, many efforts have been made to improve the treatment methods. As assisted reproductive technology is mainly using cell culture methods, one of the approaches to improve this technology is conditioned medium from different sources. It is desirable to apply in vitro maturation (IVM) and use oocytes from normal cycles instead of stimulating ovulation. OBJECTIVE: To investigate the effect of human cumulus cell condition medium (hCCCM) on the IVM of immature mouse oocytes and morphology. MATERIALS AND METHODS: In this experimental study, 240 germinal vesile oocytes were collected from four-six wk-old mice after 48 hr of 5IU pregnant mare serum gonadotropin (PMSG) injection and cultured in hCCCM (test group, n = 120) and DMEM + 20% FBS (control group, n = 120). The IVM rates and changes in perivitelline space (PVS) and shape were investigated at 8, 16, and 24 hr following the culture. The mature (MII) oocytes were subjected to in vitro fertilization (IVF) and the fertilization rate was assessed in three days. RESULTS: A significant difference was observed between the maturation rates in the hCCCM and control groups (24.16% vs 0%; p = 0.001), as well as morphologic changes between the two groups (p = 0.04, p = 0.05). The development rate for MII oocytes attained from IVM in the hCCCM group was 27.58% (2-cell) and 6.89% (4-cell). Data displayed that hCCCM is an effective medium for oocytes maturation compared to the control medium. CONCLUSION: hCCCM supports oocyte in vitro growth and maturation. Moreover, hCCCM changes the oocyte shape and size of perivitelline space. |
format | Online Article Text |
id | pubmed-7778757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Knowledge E |
record_format | MEDLINE/PubMed |
spelling | pubmed-77787572021-01-08 The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study Adib, Maryam Seifati, Seyed Morteza Ashkezari, Mahmood Dehghani Khoradmehr, Arezoo Rezaee-Ranjbar-Sardari, Roshan Tahajjodi, Somayyeh Sadat Aflatoonian, Behrouz Int J Reprod Biomed Research Article BACKGROUND: To increase the results of infertility treatment, many efforts have been made to improve the treatment methods. As assisted reproductive technology is mainly using cell culture methods, one of the approaches to improve this technology is conditioned medium from different sources. It is desirable to apply in vitro maturation (IVM) and use oocytes from normal cycles instead of stimulating ovulation. OBJECTIVE: To investigate the effect of human cumulus cell condition medium (hCCCM) on the IVM of immature mouse oocytes and morphology. MATERIALS AND METHODS: In this experimental study, 240 germinal vesile oocytes were collected from four-six wk-old mice after 48 hr of 5IU pregnant mare serum gonadotropin (PMSG) injection and cultured in hCCCM (test group, n = 120) and DMEM + 20% FBS (control group, n = 120). The IVM rates and changes in perivitelline space (PVS) and shape were investigated at 8, 16, and 24 hr following the culture. The mature (MII) oocytes were subjected to in vitro fertilization (IVF) and the fertilization rate was assessed in three days. RESULTS: A significant difference was observed between the maturation rates in the hCCCM and control groups (24.16% vs 0%; p = 0.001), as well as morphologic changes between the two groups (p = 0.04, p = 0.05). The development rate for MII oocytes attained from IVM in the hCCCM group was 27.58% (2-cell) and 6.89% (4-cell). Data displayed that hCCCM is an effective medium for oocytes maturation compared to the control medium. CONCLUSION: hCCCM supports oocyte in vitro growth and maturation. Moreover, hCCCM changes the oocyte shape and size of perivitelline space. Knowledge E 2020-12-21 /pmc/articles/PMC7778757/ /pubmed/33426412 http://dx.doi.org/10.18502/ijrm.v18i12.8023 Text en Copyright © 2020 Adib et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Article Adib, Maryam Seifati, Seyed Morteza Ashkezari, Mahmood Dehghani Khoradmehr, Arezoo Rezaee-Ranjbar-Sardari, Roshan Tahajjodi, Somayyeh Sadat Aflatoonian, Behrouz The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study |
title | The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study |
title_full | The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study |
title_fullStr | The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study |
title_full_unstemmed | The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study |
title_short | The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study |
title_sort | effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: an experimental study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778757/ https://www.ncbi.nlm.nih.gov/pubmed/33426412 http://dx.doi.org/10.18502/ijrm.v18i12.8023 |
work_keys_str_mv | AT adibmaryam theeffectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT seifatiseyedmorteza theeffectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT ashkezarimahmooddehghani theeffectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT khoradmehrarezoo theeffectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT rezaeeranjbarsardariroshan theeffectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT tahajjodisomayyehsadat theeffectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT aflatoonianbehrouz theeffectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT adibmaryam effectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT seifatiseyedmorteza effectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT ashkezarimahmooddehghani effectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT khoradmehrarezoo effectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT rezaeeranjbarsardariroshan effectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT tahajjodisomayyehsadat effectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy AT aflatoonianbehrouz effectofthehumancumuluscellsconditionedmediumoninvitromaturationofmouseoocyteanexperimentalstudy |