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The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases

Kinases form the backbone of numerous cell signaling pathways, with their dysfunction similarly implicated in multiple pathologies. Further facilitated by their druggability, kinases are a major focus of therapeutic development efforts in diseases such as cancer, infectious disease and autoimmune di...

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Autores principales: Berginski, Matthew E, Moret, Nienke, Liu, Changchang, Goldfarb, Dennis, Sorger, Peter K, Gomez, Shawn M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778917/
https://www.ncbi.nlm.nih.gov/pubmed/33079988
http://dx.doi.org/10.1093/nar/gkaa853
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author Berginski, Matthew E
Moret, Nienke
Liu, Changchang
Goldfarb, Dennis
Sorger, Peter K
Gomez, Shawn M
author_facet Berginski, Matthew E
Moret, Nienke
Liu, Changchang
Goldfarb, Dennis
Sorger, Peter K
Gomez, Shawn M
author_sort Berginski, Matthew E
collection PubMed
description Kinases form the backbone of numerous cell signaling pathways, with their dysfunction similarly implicated in multiple pathologies. Further facilitated by their druggability, kinases are a major focus of therapeutic development efforts in diseases such as cancer, infectious disease and autoimmune disorders. While their importance is clear, the role or biological function of nearly one-third of kinases is largely unknown. Here, we describe a data resource, the Dark Kinase Knowledgebase (DKK; https://darkkinome.org), that is specifically focused on providing data and reagents for these understudied kinases to the broader research community. Supported through NIH’s Illuminating the Druggable Genome (IDG) Program, the DKK is focused on data and knowledge generation for 162 poorly studied or ‘dark’ kinases. Types of data provided through the DKK include parallel reaction monitoring (PRM) peptides for quantitative proteomics, protein interactions, NanoBRET reagents, and kinase-specific compounds. Higher-level data is similarly being generated and consolidated such as tissue gene expression profiles and, longer-term, functional relationships derived through perturbation studies. Associated web tools that help investigators interrogate both internal and external data are also provided through the site. As an evolving resource, the DKK seeks to continually support and enhance knowledge on these potentially high-impact druggable targets.
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spelling pubmed-77789172021-01-06 The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases Berginski, Matthew E Moret, Nienke Liu, Changchang Goldfarb, Dennis Sorger, Peter K Gomez, Shawn M Nucleic Acids Res Database Issue Kinases form the backbone of numerous cell signaling pathways, with their dysfunction similarly implicated in multiple pathologies. Further facilitated by their druggability, kinases are a major focus of therapeutic development efforts in diseases such as cancer, infectious disease and autoimmune disorders. While their importance is clear, the role or biological function of nearly one-third of kinases is largely unknown. Here, we describe a data resource, the Dark Kinase Knowledgebase (DKK; https://darkkinome.org), that is specifically focused on providing data and reagents for these understudied kinases to the broader research community. Supported through NIH’s Illuminating the Druggable Genome (IDG) Program, the DKK is focused on data and knowledge generation for 162 poorly studied or ‘dark’ kinases. Types of data provided through the DKK include parallel reaction monitoring (PRM) peptides for quantitative proteomics, protein interactions, NanoBRET reagents, and kinase-specific compounds. Higher-level data is similarly being generated and consolidated such as tissue gene expression profiles and, longer-term, functional relationships derived through perturbation studies. Associated web tools that help investigators interrogate both internal and external data are also provided through the site. As an evolving resource, the DKK seeks to continually support and enhance knowledge on these potentially high-impact druggable targets. Oxford University Press 2020-10-20 /pmc/articles/PMC7778917/ /pubmed/33079988 http://dx.doi.org/10.1093/nar/gkaa853 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Database Issue
Berginski, Matthew E
Moret, Nienke
Liu, Changchang
Goldfarb, Dennis
Sorger, Peter K
Gomez, Shawn M
The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases
title The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases
title_full The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases
title_fullStr The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases
title_full_unstemmed The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases
title_short The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases
title_sort dark kinase knowledgebase: an online compendium of knowledge and experimental results of understudied kinases
topic Database Issue
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778917/
https://www.ncbi.nlm.nih.gov/pubmed/33079988
http://dx.doi.org/10.1093/nar/gkaa853
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